Filter Buffy Coats (FBC): A source of peripheral blood leukocytes recovered from leukocyte depletion filters

Meyer, Thomas; Zehnter, I.; Bt, Hofmann; Zaisserer, J.; Burkhart, Jürgen; Rapp, S.; Weinauer, Franz; Schmitz, John L.; Illert, W.E.

doi:10.1016/j.jim.2005.10.004

Cited by 84 publications

(77 citation statements)

References 18 publications

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“…Leukocytes were collected from freshly (2-3 h) prepared leukocyte depletion filters (Sepacell RZ 2000) of blood from healthy blood donors according to a previously published method (32). Mo or CD4 + naive T cells were isolated using MACS, according to the manufacturer's instructions (Monocyte isolation kit II, Naive CD4 + T cell isolation kit II; Miltenyi Biotec, Bergisch Gladbach, Germany).…”

Section: Isolation Of Mo and Cd4 + T Cellsmentioning

confidence: 99%

Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients

Bergenfelz

Medrek

Ekström

et al. 2012

The Journal of Immunology

108

101

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A well-orchestrated inflammatory reaction involves the induction of effector functions and, at a later stage, an active downregulation of this potentially harmful process. In this study we show that under proinflammatory conditions the noncanonical Wnt protein, Wnt5a, induces immunosuppressive macrophages. The suppressive phenotype induced by Wnt5a is associated with induction of IL-10 and inhibition of the classical TLR4-NF-κB signaling. Interestingly, this phenotype closely resembles that observed in reprogrammed monocytes in sepsis patients. The Wnt5a-induced feedback inhibition is active both during in vitro LPS stimulation of macrophages and in patients with sepsis caused by LPS-containing, Gram-negative bacteria. Furthermore, using breast cancer patient tissue microarrays, we find a strong correlation between the expression of Wnt5a in malignant epithelial cells and the frequency of CD163+ anti-inflammatory tumor-associated macrophages. In conclusion, our data point out Wnt5a as a potential target for an efficient therapeutic modality in severe human diseases as diverse as sepsis and malignancy.

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Section: Isolation Of Mo and Cd4 + T Cellsmentioning

confidence: 99%

Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients

Bergenfelz

Medrek

Ekström

et al. 2012

The Journal of Immunology

108

101

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“…Dendritic cells were generated from filter buffy coats (FBC)-derived monocytes donated by healthy human donors (Meyer et al, 2005). The protocol for obtention of FBC was approved by the Ethics Committee of our institution and was carried out on donors who gave informed consent.…”

Section: Generation and Maturationmentioning

confidence: 99%

Influence of bevacizumab, sunitinib and sorafenib as single agents or in combination on the inhibitory effects of VEGF on human dendritic cell differentiation from monocytes

et al. 2009

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Vascular endothelial growth factor (VEGF) inhibits differentiation and maturation of dendritic cells (DC), suggesting a potential immunosuppressive role for this proangiogenic factor. Bevacizumab, sorafenib and sunitinib target VEGF-mediated angiogenesis and are active against several types of cancer, but their effects on the immune system are poorly understood. In this study, VEGF and supernatants of renal carcinoma cell lines cultured under hypoxia were found to alter the differentiation of human monocytes to DC. Resulting DC showed impaired activity, as assessed by the alloreactive mixed T-lymphocyte reaction. Bevacizumab and sorafenib, but not sunitinib, reversed the inhibitory effects of VEGF, but not of those mediated by tumour supernatants. Dendritic cells matured under the influence of VEGF expressed less human leukocyte antigen-DR (HLA-DR) and CD86, and this effect was restored by bevacizumab and sorafenib. Finally, tumour-cell supernatants decreased interleukin-12 (IL-12) production by mature DC, and such inhibition was not restored by any of the tested drugs, delivered either as single agents or in combination. The deleterious effects of tumour-cell supernatants were mainly mediated by thermostable molecules distinct from VEGF. These results indicate that inhibition of the differentiation of monocytes to DC is a multifactorial effect, and that they support the development of combinations of angiogenesis inhibitors with immunological modulators.

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“…Furthermore, pDCs isolated from peripheral blood using anti-BDCA-4 all showed strong staining for CD2AP (Figure 4) and CD123. BDCA-4 is a well-accepted marker for purification of pDCs, 32,33 and it was used previously by 2 authors of this paper (F.F. and S.S.) to purify pDCs, achieving a purity of 90% to 98%.…”

Section: Pdcs In Biopsies From Reactive Conditionsmentioning

confidence: 99%

Novel markers of normal and neoplastic human plasmacytoid dendritic cells

Marafioti

Paterson

Ballabio

et al. 2008

Blood

202

169

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Filter Buffy Coats (FBC): A source of peripheral blood leukocytes recovered from leukocyte depletion filters

Cited by 84 publications

References 18 publications

Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients

Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients

Influence of bevacizumab, sunitinib and sorafenib as single agents or in combination on the inhibitory effects of VEGF on human dendritic cell differentiation from monocytes

Novel markers of normal and neoplastic human plasmacytoid dendritic cells

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