Filter Capacity Predictions for the Capture of Magnetic Microparticles by High-Gradient Magnetic Separation

Ebner, N.A.; Gomes, Claudia Sofia Goncalves; Hobley, Timothy John; Thomas, Owen R.T.; Franzreb, Matthias

doi:10.1109/tmag.2007.892080

Cited by 21 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this purpose, custom‐made prototype magnetic separators have been developed with a wide variety of approaches of shape, sizes, and magnetic background field but still following the classical fixed matrix design. Rolled or staged wire meshes or filamentary rod constructions are favored as a defined matrix structure . The use of these matrix designs results in good separation performances with over 90% of MP separation from the feed‐streams, but the resuspension efficiency needed in order to wash and recover the MP from the system for further use has been mostly ignored.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Separation on a New Level: Characterization and Performance Prediction of a cGMP Compliant “Rotor‐Stator” High‐Gradient Magnetic Separator

Ebeler

Pilgram

Wolz

et al. 2017

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

The growing market of biopharmaceuticals and the constant developments in upstream fermentation have generated a strong demand for new downstream purification methods. Magnetic separation in combination with functional magnetic particles has been known for many years as a promising candidate for a direct capturing tool in protein purification but the lack of suitable GMP-compliant purification equipment has prevented the launch of this technology in large scale bioprocessing. To tackle this bottle-neck, the principle of a "rotor-stator" high-gradient magnetic separator is fully redesigned to meet the rigorous requirements of modern cGMP biotechnology purification processes. In order to fulfill regulatory requirements, the separation chamber is reengineered to allow effective cleaning and sterilization in place while maintaining excellent separation capacities and efficiencies. Two kinds of commercially available magnetic particles are used to validate key performance data and determine system related parameters in order to calculate process performance figures for process optimization of the new magnetic separation device. With separation capacities of over 400 g of magnetic particles per liter of separation chamber volume and separation efficiencies as well as recovery rates over 99%, the system is able to process more than 200 l crude feedstock per day and capture more than 1.6 kg target compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Magnetic Separation on a New Level: Characterization and Performance Prediction of a cGMP Compliant “Rotor‐Stator” High‐Gradient Magnetic Separator

Ebeler

Pilgram

Wolz

et al. 2017

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…In several prior studies, the retention of magnetic particles on different matrices has been analyzed. In for example filter matrices without movable parts were studied. The magnetic separator that corresponds to the rotor‐stator principle was used usually with circular filter plates and rounded notches .…”

Section: Introductionmentioning

confidence: 99%

Optimizing a rotor‐stator filter matrix for high‐gradient magnetic separation of functionalized magnetic particles

Shaikh

Seibert

Schumann

et al. 2016

Engineering in Life Sciences

View full text Add to dashboard Cite

The processing of wines with enzymes is a process chain in which losses of biocatalyst are unavoidable. A promising technique for the minimization of these losses and for the reduction of processing time is the high-gradient magnetic separation in combination with enzymes, which are immobilized onto functionalized magnetic particles. When magnetizable particles are used and magnetic separation is applied to separate these particles from nonmagnetizable particles and solutes, the enzymes can be recycled and used for several production batches. The magnetic filter used in this study had a filter matrix with concentrically stacked circular rotor and stator plates which are arranged in an alternating order. Different geometries of the filter plate notches were examined to optimize the reproducibility of particle retention. In computational fluid dynamic studies, the influence of the notch geometries on the shear rate generation was analyzed for the rinsing procedure. Separation experiments with an optimized geometry of the filter plates were carried out in water and white wine suspensions.

show abstract

“…Previously, matrices of different size or shapes, different arrangements were manufactured to do experiments to optimize the HGMS system (Too et al, 1986;Badescu et al, 1996;Ebner et al, 2007;Kim et al, 2013;Padmanabhan and Sreenivas, 2011;Chen et al, 2014). However, as there are so many factors influencing the recovery efficiency, the quantitative analyses of these factors were not done systematically.…”

Section: Introductionmentioning

confidence: 99%

Study on capture radius and efficiency of fine weakly magnetic minerals in high gradient magnetic field

Zheng

Wang

2015

Minerals Engineering

View full text Add to dashboard Cite

Filter Capacity Predictions for the Capture of Magnetic Microparticles by High-Gradient Magnetic Separation

Cited by 21 publications

References 13 publications

Magnetic Separation on a New Level: Characterization and Performance Prediction of a cGMP Compliant “Rotor‐Stator” High‐Gradient Magnetic Separator

Magnetic Separation on a New Level: Characterization and Performance Prediction of a cGMP Compliant “Rotor‐Stator” High‐Gradient Magnetic Separator

Optimizing a rotor‐stator filter matrix for high‐gradient magnetic separation of functionalized magnetic particles

Study on capture radius and efficiency of fine weakly magnetic minerals in high gradient magnetic field

Contact Info

Product

Resources

About