Filtration, adsorption and immunodetection of virus using polyelectrolyte multilayer-modified paper

Larsson, Per Tomas; Puttaswamaiah, Sushma G.; Ly, Christine; Vanerek, Alois; Hall, J. Christopher; Drolet, François

doi:10.1016/j.colsurfb.2012.06.023

Cited by 19 publications

(19 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Researchers have demonstrated that paper based direct ELISA can detect antibody (rabbit IgG), HIV-1 antigens, bacteria and viruses [16,18,20,32,44]. Scientists have reported detecting M13 viruses using paper based direct ELISA method, which requires a sophisticated setup including a Bio-Dot filtration apparatus, an ELISA microtitre plate, and a vacuum pump [20]. However, the direct ELISA technique has a lower sensitivity [32].…”

Section: Discussionmentioning

confidence: 99%

“…However, the direct ELISA technique has a lower sensitivity [32]. The limit of detection (LOD) of the modified paper based direct ELISA technique was 10 6 -10 7 pfu/mL; to improve the sensitivity (as low as 10 4 pfu/mL) of the technique, high sample volume (100 mL) was required [20]. A paper based diagnosis needs to be sensitive, should be simple in performance and require fewer steps, and should have the capacity of being performed at rural areas with minimum resources and training [1,4,18].…”

Section: Discussionmentioning

confidence: 99%

“…Paper has the potential to meet these criteria. Researchers demonstrated that antibody and enzyme active papers can detect ABO blood typing [1], bacteria [11], DNA [12], alcohol content in the breath [13], seafood freshness [14], and pathogens in biofluids [15][16][17][18][19][20]. However, simplicity, sensitivity, and amount of biofluid volume required for the test are ongoing challenges for developing paper based devices to detect pathogens, such as viruses [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Qualitative and quantitative detection of T7 bacteriophages using paper based sandwich ELISA

Khan

Pande

Ven

2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Qualitative and quantitative detection of T7 bacteriophages using paper based sandwich ELISA

Khan

Pande

Ven

2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

“…Nowadays, 160 million tons of pulp are produced worldwide every year, which are mainly used for the manufacturing of a variety of paper grades ranging from ordinary papers, e.g., writing and packaging paper, to specialty papers like filters and electrical papers . In order to enter new fields of application, pulp fibers can be endowed with novel functionalities using different chemical, enzymatic, or physical treatments . From our point of view, the water‐based adsorption of cationic polysaccharides is one of the most promising ways to implement new properties to pulp fibers .…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5] In order to enter new fields of application, pulp fibers can be endowed with novel functionalities using different chemical, enzymatic, or physical treatments. [6][7][8][9][10][11][12][13] From our point of view, the water-based adsorption of cationic polysaccharides is one of the most promising ways to implement new properties to pulp fibers. [14,15] This method allows to separate the challenging step of direct chemical modification of fibers from the fiber line.…”

Section: Introductionmentioning

confidence: 99%

Photocontrol of Mechanical Properties of Pulp Fibers and Fiber‐to‐Fiber Bonds via Self‐Assembled Polysaccharide Derivatives

Grigoray

Wondraczek

Daus

et al. 2014

Macro Materials & Eng

View full text Add to dashboard Cite

Photoresponsive pulp fibers are prepared by self‐assembly of photoactive cationic cellulose derivatives with pulp fibers in aqueous environment. Photoactive groups of the derivatives undergo 2π + 2π cycloaddition reaction under UV‐light irradiation. Fast photocrosslinking leads to the formation of the covalent bonds between the photoactive groups on the fiber surfaces. This results in drastic enhancement of the mechanical properties of the fiber network. Tensile strength and Z‐directional tensile strength increase by 81 and 84% compared to the original fiber network. Stiffness of the individual fibers increases by 60%. Such concept of controlling mechanical properties of the fiber materials by the light gives a possibility to design smart bio‐based materials and to increase end value of the fiber products.

show abstract

Immunochemical biosensor for single virus particle detection based on molecular crowding polyelectrolyte system

Baldina

Nikolaev

Ivanov

et al. 2022

J of Applied Polymer Sci

View full text Add to dashboard Cite

To improve the effectiveness of the viral infection diagnostic, we offer a new approach of immunochemical biosensors to determine single viral particles by specific antibodies. The antibodies are immobilized on the electrodes in a three-dimensional polymer matrix with several layers of polyelectrolytes on the screen-printed carbon electrode. Non-covalent immobilization of antibodies in successive layers of positively charged polyethyleneimine (PEI) and negatively charged polystyrene sulfonate (PSS) achieves the effect of macromolecular crowding. Such an immobilization approach promotes the preservation of the optimal conformation and antibody active center mobility for interaction with large virion particles. We established an electrochemical biosensor for tickborne encephalitis virus (TBEV) detection to demonstrate the method's applicability. Under the optimized architecture of the 3D-matrix, including a combination of two layers of a positively charged PEI with antibodies and the last layer of a negatively charged PSS, the assay is characterized by an extremely low limit of detection (LOD). This LOD could be as few as five viral particles in a sample volume of 5 μl, which is two orders of magnitude lower than conventional ELISA with the same reagents. The advantage of the biosensor is also a wide linear range of detection from 10 3 to 10 9 viral particles/ml. The proposed principle for determining virion particles is well suited to novel express diagnostics and Point-of-Care viral infections detection.

show abstract

Filtration, adsorption and immunodetection of virus using polyelectrolyte multilayer-modified paper

Cited by 19 publications

References 28 publications

Qualitative and quantitative detection of T7 bacteriophages using paper based sandwich ELISA

Qualitative and quantitative detection of T7 bacteriophages using paper based sandwich ELISA

Photocontrol of Mechanical Properties of Pulp Fibers and Fiber‐to‐Fiber Bonds via Self‐Assembled Polysaccharide Derivatives

Immunochemical biosensor for single virus particle detection based on molecular crowding polyelectrolyte system

Contact Info

Product

Resources

About