Filtration pressure and red blood cell deformability: Evaluation of a new device: erythrometer

Guéguen, M.; Jm, Bidet; Durand, F.; Driss, Françoise; Joffre, A; Genetet, B

doi:10.3233/bir-1984-23s145

Cited by 19 publications

(8 citation statements)

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“…In the case of the cell transit through pores, the shear stress is strictly related to the size-pore/cellsize ratio, although the driving force and shape of pore edges are also important [31,48]. It has been calculated, for example, that the nominal shear stress generated at the wall of a pore of 5 µm diameter at a pressure gradient of 3 cm H 2 O is 24 Pa (the Cell Transit Analyzer, CTA -the system with an added conductimeter measuring alterations in electrical resistance on the filter during the RBC passage) [4].…”

Section: Flow Systemsmentioning

confidence: 99%

Red blood cell-deformability measurement: Review of techniques

Musielak¹

2009

Clinical Hemorheology and Microcirculation

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Cell-deformability characterization involves general measurement of highly complex relationships between cell biology and physical forces to which the cell is subjected. The review takes account of the modern technical solutions simulating the action of the force applied to the red blood cell in macro-and microcirculation. Diffraction ektacytometers and rheoscopes measure the mean deformability value for the total red blood cell population investigated and the deformation distribution index of individual cells, respectively. Deformation assays of a whole single cell are possible by means of optical tweezers. The single cell-measuring setups for micropipette aspiration and atomic force microscopy allow conducting a selective investigation of deformation parameters (e.g., cytoplasm viscosity, viscoelastic membrane properties). The distinction between instrument sensitivity to various RBC-rheological features as well as the influence of temperature on measurement are discussed. The reports quoted confront fascinating possibilities of the techniques with their medical applications since the RBC-deformability has the key position in the etiology of a wide range of conditions.

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Section: Flow Systemsmentioning

confidence: 99%

Red blood cell-deformability measurement: Review of techniques

Musielak¹

2009

Clinical Hemorheology and Microcirculation

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“…Various methods for measuring RBC deformability exist; these include filtration through small pore filters (Gueguen et al, 1984), viscometry of cell suspensions, aspiration of cells into micropipets (Engstrom and Meiselman, 1996), and deformation of cells under fluid shear stress. Fluid shear assays include Ektacytometry (Bessis and Mohandas, 1975;Bessis et al, 1980) and Rheoscopy (Dobbe et al, 2002(Dobbe et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels

Korin

Bransky

Dinnar

2007

Journal of Biomechanics

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“…Some examples are the RBC filtration [6], laser diffraction ellipsometry [7], rheoscopy [8], micropipette aspiration [9] and cytometry [10]. Recently, by using a soft lithography technique it is possible to fabricate transparent microchannels to investigate the motion and dynamical deformation of cells [3,[11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

et al. 2014

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Red blood cell (RBC) deformability has become one of the important factors to assess blood and cardiovascular diseases. The interest on blood studies have promoted a development of various microfluidic devices that treat and analyse blood cells. Recent years, besides the RBC deformability assessment, these devices are often applied to cancer cell detection and isolation from the whole blood. The devices for cancer cell isolation rely mainly on size and deformability of the cells. However, the examination of deformability of the RBCs mixed with cancer cells is lacking. This study aims at determining the deformation index (DI) of the RBCs in contact with cancer cells using a hyperbolic microchannel which generates a strong extensional flow. The DIs of human healthy RBCs and human RBCs in contact with a tumor cell line (HCT-15, colon carcinoma) were compared by analyzing the flowing RBCs images captured by a high speed camera. The results reveal that the RBCs that were in contact with HCT-15 cells have lower deformability than the normal RBCs.

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Filtration pressure and red blood cell deformability: Evaluation of a new device: erythrometer

Cited by 19 publications

References 0 publications

Red blood cell-deformability measurement: Review of techniques

Red blood cell-deformability measurement: Review of techniques

Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels

Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

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