Filtration Supports for Affinity Separation

Mandaro, R.; Roy, Sujata; Hou, Kenneth C.

doi:10.1038/nbt0987-928

Cited by 26 publications

(9 citation statements)

References 6 publications

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“…Initially, membrane adsorbers were primarily seen as excellent stationary phases for affinity filtration [8,9]. Most approaches known from filtration technology were also used with the functionalized units, including dead end filtration and cross flow filtration, for example in hollow fiber units.…”

Section: From Interactive Membranes To "Membranementioning

confidence: 99%

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Tennikova

Freitag

2000

J. High Resol. Chromatogr.

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Section: From Interactive Membranes To "Membranementioning

confidence: 99%

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Tennikova

Freitag

2000

J. High Resol. Chromatogr.

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“…These membranes possessed high porosity and good flow characteristics. Because of the presence of active epoxides, these membranes could be further modified with ion‐exchange groups, such as diethylaminoethyl (DEAE) or sulfopropyl (SP), and affinity ligands (protein A or protein G) and used for purification of various proteins (such as interleukin‐1 factor, recombinant protein, monoclonal antibodies, immunoglobulins, tissue plasminogen, and clotting factors) (77–80).…”

Section: Preparation Methods Of Adsorptive Membranesmentioning

confidence: 99%

Membrane Chromatography: Preparation and Applications to Protein Separation

1999

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As a result of the convective flow of solutes through porous membranes, membrane chromatography has a higher capture efficiency and a higher productivity than column chromatography and shows most promising industrial applications for the recovery, isolation, and purification of proteins and enzymes. This paper presents a comprehensive review of the methods for preparation of adsorptive membranes (such as surface modification, in situ copolymerization, direct formation from hydrophilic materials, and functionalized particulate-entrapped membranes) and deals particularly with novel macroporous chitin and chitosan membranes for protein separations developed by the authors.

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“…Pre-separation steps Pre-separation steps Solid-liquid separation Protein depletion or protein fractionation by any of the separation techniques (a) Filtration [180] (b) Centrifugation [181] Precipitation (a) Salt [182] (b) Organic solvent [183] (c) Polymer [184] (d) Detergent [185] Chromatographic steps Identification steps Ion exchange chromatography Two-dimensional gel electrophoresis Hydrophobic interaction chromatography [186] Mass spectrometry (coupled to liquid chromatography) Affinity chromatography [187] Orthogonal chromatography Gel filtration chromatography [188] Two-dimensional chromatography Radial flow chromatography [189] Direct analysis of large protein complexes (DALPC) Perfusion chromatography [190] Isoelectric focusing using nonporous reverse phase HPLC (IEF-NP RP HPLC) Expanded bed chromatography [42,43] Displacement chromatography [33,34] Bioimaging Monoliths [191] Microarray profiling Non-chromatographic steps Microfluidics Aqueous two phase extraction [67][68][69] Three phase partitioning [63,64] Reverse micellar extraction [192] Crossflow ultrafiltration [193] Preparative electrophoresis [194] The techniques listed here are illustrative and not all-inclusive. In the 'proteomics' scenario, separation is often synonymous with identification.…”

Section: Conventional Separation Proteomic Separationmentioning

confidence: 99%