Final report on international comparison CCQM-K68: Nitrous oxide in synthetic air

Lee, Jeongsoon; Moon, D. W.; Kim, Jin Seog; Wessel, Rob M; Aoki, Nobuyuki; Kato, Kenji; Guenther, Frank; Rhoderick, George C.; Konopelko, L. A.; Han, Qiao; Hall, B. D.

doi:10.1088/0026-1394/48/1a/08004

Cited by 13 publications

(13 citation statements)

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“…We used six certified reference gas mixtures of N 2 O in nitrogen-0.198, 0.331, 0.550, 0.798, 12.0, and 94.9 mol N 2 O/mol-to calibrate the GC/ ECD. These reference gas mixtures were gravimetrically prepared by the Korea Research Institute for Standards and Science and verified by international comparisons, CCQM-K68, in 2010 (46). The uncertainty in the N 2 O concentration was ca.…”

Section: Methodsmentioning

confidence: 99%

Enrichment and Characterization of an Autotrophic Ammonia-Oxidizing Archaeon of Mesophilic Crenarchaeal Group I.1a from an Agricultural Soil

Jung

Park

Min

et al. 2011

Appl Environ Microbiol

245

222

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Soil nitrification is an important process for agricultural productivity and environmental pollution. Though one cultivated representative of ammonia-oxidizing Archaea from soil has been described, additional representatives warrant characterization. We describe an ammonia-oxidizing archaeon (strain MY1) in a highly enriched culture derived from agricultural soil. Fluorescence in situ hybridization microscopy showed that, after 2 years of enrichment, the culture was composed of >90% archaeal cells. Clone libraries of both 16S rRNA and archaeal amoA genes featured a single sequence each. No bacterial amoA genes could be detected by PCR. A [ 13 C]bicarbonate assimilation assay showed stoichiometric incorporation of 13 C into Archaeaspecific glycerol dialkyl glycerol tetraethers. Strain MY1 falls phylogenetically within crenarchaeal group I.1a; sequence comparisons to "Candidatus Nitrosopumilus maritimus" revealed 96.9% 16S rRNA and 89.2% amoA gene similarities. Completed growth assays showed strain MY1 to be chemoautotrophic, mesophilic (optimum at 25°C), neutrophilic (optimum at pH 6.5 to 7.0), and nonhalophilic (optimum at 0.2 to 0.4% salinity). Kinetic respirometry assays showed that strain MY1's affinities for ammonia and oxygen were much higher than those of ammonia-oxidizing bacteria (AOB). The yield of the greenhouse gas N 2 O in the strain MY1 culture was lower but comparable to that of soil AOB. We propose that this new soil ammonia-oxidizing archaeon be designated "Candidatus Nitrosoarchaeum koreensis."

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Section: Methodsmentioning

confidence: 99%

Enrichment and Characterization of an Autotrophic Ammonia-Oxidizing Archaeon of Mesophilic Crenarchaeal Group I.1a from an Agricultural Soil

Jung

Park

Min

et al. 2011

Appl Environ Microbiol

245

222

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“…CCLs are encouraged to compare the WMO-GAW scales to other independent scales whenever possible, e.g. through participation in key comparisons organised by BIPM [22,23] and other exercises as appropriate. All WMO measurements are made traceable to an appropriate scale [30,53], which leads to high internal consistency of data within and between networks.…”

Section: Quality Assurance and Quality Controlmentioning

confidence: 99%

Advances in reference materials and measurement techniques for greenhouse gas atmospheric observations

et al. 2019

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We present the global research landscape which aims to deliver a measurement infrastructure to underpin atmospheric observations of key greenhouse gases governing changes in the Earth's climate. These measurements present a significant challenge to the metrological community, analytical laboratories and major producers of reference materials. The review focuses on the progress made in the Gas Analysis Working Group of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology (CCQM-GAWG) in establishing the primary realisation of the amount-of-substance fraction for carbon dioxide, methane and nitrous oxide in an air matrix. It also focuses on the importance of providing traceable measurements of isotopic composition of these components for commutability of reference materials and for isotope ratio measurements for greenhouse gas source attribution. Metrologia 56 (2019) 034006 (29pp) P J Brewer et al 2 of the World Meteorological Organization (WMO) for providing the framework for the development and implementation of integrated greenhouse gas observations, which is vital for understanding the global carbon cycle and the role greenhouse gases play in climate change. The developments in analytical techniques are also discussed which have shaped the direction of the metrology required to meet the evolving and future needs of stakeholders.

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“…We used six certified reference gas mixtures of N 2 O in nitrogen, that is, 0.198, 0.331, 0.550, 0.798, 12.0, and 94.9 N 2 O mmol mol À 1 , to calibrate the GC/QMS. These reference gas mixtures were prepared gravimetrically by the Korea Research Institute for Standards and Science and verified by international comparisons, that is, CCQM-K68 in 2010 (Lee et al, 2011).…”

Section: N 2 O Tracer Experimentsmentioning

confidence: 99%

Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

Well²,

et al. 2013

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N 2 O gas is involved in global warming and ozone depletion. The major sources of N 2 O are soil microbial processes. Anthropogenic inputs into the nitrogen cycle have exacerbated these microbial processes, including nitrification. Ammonia-oxidizing archaea (AOA) are major members of the pool of soil ammonia-oxidizing microorganisms. This study investigated the isotopic signatures of N 2 O produced by soil AOA and associated N 2 O production processes. All five AOA strains (I.1a, I.1a-associated and I.1b clades of Thaumarchaeota) from soil produced N 2 O and their yields were comparable to those of ammonia-oxidizing bacteria (AOB). The levels of site preference (SP), d 15 N bulk and d 18 O -N 2 O of soil AOA strains were 13-30%, À 13 to À 35% and 22-36%, respectively, and strains MY1-3 and other soil AOA strains had distinct isotopic signatures. A 15 N-NH 4 þ -labeling experiment indicated that N 2 O originated from two different production pathways (that is, ammonia oxidation and nitrifier denitrification), which suggests that the isotopic signatures of N 2 O from AOA may be attributable to the relative contributions of these two processes. The highest N 2 O production yield and lowest site preference of acidophilic strain CS may be related to enhanced nitrifier denitrification for detoxifying nitrite. Previously, it was not possible to detect N 2 O from soil AOA because of similarities between its isotopic signatures and those from AOB. Given the predominance of AOA over AOB in most soils, a significant proportion of the total N 2 O emissions from soil nitrification may be attributable to AOA.

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Final report on international comparison CCQM-K68: Nitrous oxide in synthetic air

Cited by 13 publications

References 0 publications

Enrichment and Characterization of an Autotrophic Ammonia-Oxidizing Archaeon of Mesophilic Crenarchaeal Group I.1a from an Agricultural Soil

Enrichment and Characterization of an Autotrophic Ammonia-Oxidizing Archaeon of Mesophilic Crenarchaeal Group I.1a from an Agricultural Soil

Advances in reference materials and measurement techniques for greenhouse gas atmospheric observations

Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

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