Fine mapping and characterization of the awn inhibitor B1 locus in common wheat (Triticum aestivum L.)

Niu, Jianqing; Zheng, Shusong; Shi, Xiaoli; Si, Yaoqi; Tian, Shuiquan; He, Yilin; Ling, Hong‐Qing

doi:10.1016/j.cj.2019.12.005

Cited by 18 publications

(18 citation statements)

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“…These genes affect various spike traits including spike length, spike morphology, grain size and shape, but since all known common wheats have QcS genotype, it is likely that there are other genes which contribute to spike morphology [ 53 ]. For awn length, three dominant awn length inhibitors, Hooded ( Hd ), Tipped1 ( B1 ), and Tipped2 ( B2 ) are known but their functions have not been characterized yet [ 54 ]. TraesCS2D01G036800 (Cullin-associated NEDD8-dissociated protein 1) has not been widely studied, but in tomato, the suppression of this gene resulted in several phenotypic changes, including dwarfism, early flowering, and suppression of seed germination [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of genetic loci associated with major agronomic traits of wheat (Triticum aestivum L.) based on genome-wide association analysis

et al. 2021

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Background Bread wheat (Triticum aestivum L.) is one of the most widely consumed cereal crops, but its complex genome makes it difficult to investigate the genetic effect on important agronomic traits. Genome-wide association (GWA) analysis is a useful method to identify genetic loci controlling complex phenotypic traits. With the RNA-sequencing based gene expression analysis, putative candidate genes governing important agronomic trait can be suggested and also molecular markers can be developed. Results We observed major quantitative agronomic traits of wheat; the winter survival rate (WSR), days to heading (DTH), days to maturity (DTM), stem length (SL), spike length (SPL), awn length (AL), liter weight (LW), thousand kernel weight (TKW), and the number of seeds per spike (SPS), of 287 wheat accessions from diverse country origins. A significant correlation was observed between the observed traits, and the wheat genotypes were divided into three subpopulations according to the population structure analysis. The best linear unbiased prediction (BLUP) values of the genotypic effect for each trait under different environments were predicted, and these were used for GWA analysis based on a mixed linear model (MLM). A total of 254 highly significant marker-trait associations (MTAs) were identified, and 28 candidate genes closely located to the significant markers were predicted by searching the wheat reference genome and RNAseq data. Further, it was shown that the phenotypic traits were significantly affected by the accumulation of favorable or unfavorable alleles. Conclusions From this study, newly identified MTA and putative agronomically useful genes will help to study molecular mechanism of each phenotypic trait. Further, the agronomically favorable alleles found in this study can be used to develop wheats with superior agronomic traits.

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Section: Discussionmentioning

confidence: 99%

Identification of genetic loci associated with major agronomic traits of wheat (Triticum aestivum L.) based on genome-wide association analysis

et al. 2021

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“…This linkage has also complicated the fine mapping and gene cloning of Lks1. The awn inhibitor gene B1, which is dominant and inhibits awn development, has recently been cloned in wheat by several independent groups [48][49][50][51][52]. The wheat B1 gene encodes a putative C2H2 zinc-finger protein with an EAR domain that is characteristic of transcriptional repressors.…”

Section: Mapped Genetic Loci For Awns In Barleymentioning

confidence: 99%

Genetic Loci Underlying Awn Morphology in Barley

Huang

Hong

2021

Genes

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Barley awns are highly active in photosynthesis and account for 30–50% of grain weight in barley. They are diverse in length, ranging from long to awnless, and in shape from straight to hooded or crooked. Their diversity and importance have intrigued geneticists for several decades. A large collection of awnness mutants are available—over a dozen of them have been mapped on chromosomes and a few recently cloned. Different awnness genes interact with each other to produce diverse awn phenotypes. With the availability of the sequenced barley genome and application of new mapping and gene cloning strategies, it will now be possible to identify and clone more awnness genes. A better understanding of the genetic basis of awn diversity will greatly facilitate development of new barley cultivars with improved yield, adaptability and sustainability.

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“…Awns play important roles in seed dispersal and crop production. The awns of wheat carry the abilities of photosynthesis and carbon exchange [ 24 ]. Up to date, three loci B1 ( Tipped 1 ), B2 ( Tipped 2 ), and Hd ( Hooded ) were reported as dominant suppressors for awn development [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…The awns of wheat carry the abilities of photosynthesis and carbon exchange [ 24 ]. Up to date, three loci B1 ( Tipped 1 ), B2 ( Tipped 2 ), and Hd ( Hooded ) were reported as dominant suppressors for awn development [ 24 , 25 ]. At least one of them, B1, can be attributed to InDels for their origination [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

InDels Identification and Association Analysis with Spike and Awn Length in Chinese Wheat Mini-Core Collection

Wang

Deng

Kong

et al. 2022

IJMS

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Diversity surveys of germplasm are important for gaining insight into the genomic basis for crop improvement; especially InDels, which are poorly understood in hexaploid common wheat. Here, we describe a map of 89,923 InDels from exome sequencing of 262 accessions of a Chinese wheat mini-core collection. Population structure analysis, principal component analysis and selective sweep analysis between landraces and cultivars were performed. Further genome-wide association study (GWAS) identified five QTL (Quantitative Trait Loci) that were associated with spike length, two of them, on chromosomes 2B and 6A, were detected in 10 phenotypic data sets. Assisted with RNA-seq data, we identified 14 and 21 genes, respectively that expressed in spike and rachis within the two QTL regions that can be further investigated for candidate genes discovery. Moreover, InDels were found to be associated with awn length on chromosomes 5A, 6B and 4A, which overlapped with previously reported genetic loci B1 (Tipped 1), B2 (Tipped 2) and Hd (Hooded). One of the genes TaAGL6 that was previously shown to affect floral organ development was found at the B2 locus to affect awn length development. Our study shows that trait-associated InDels may contribute to wheat improvement and may be valuable molecular markers for future wheat breeding

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Fine mapping and characterization of the awn inhibitor B1 locus in common wheat (Triticum aestivum L.)

Cited by 18 publications

References 50 publications

Identification of genetic loci associated with major agronomic traits of wheat (Triticum aestivum L.) based on genome-wide association analysis

Identification of genetic loci associated with major agronomic traits of wheat (Triticum aestivum L.) based on genome-wide association analysis

Genetic Loci Underlying Awn Morphology in Barley

InDels Identification and Association Analysis with Spike and Awn Length in Chinese Wheat Mini-Core Collection

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