Fine Mapping and Marker Development for the Crossability Gene <i>SKr</i> on Chromosome 5BS of Hexaploid Wheat (<i>Triticum aestivum</i> L.)

Alfares, Walid; Bouguennec, Annaig; Balfourier, François; Bergès, Hélène; Vautrin, Sonia; Sourdille, Pierre; Bernard, Michel; Feuillet, Catherine

doi:10.1534/genetics.109.107706

Cited by 38 publications

(36 citation statements)

References 69 publications

(85 reference statements)

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“…Two SSR markers completely linked to SKr were used to evaluate a collection of crossable wheat progenies originating from primary triticale breeding programmes. The results confirm the major effect of SKr on crossability and the usefulness of the two markers for the efficient introgression of crossability into elite wheat varieties (Alfares et al 2009). …”

Section: Wheat × Rye Crossabilitysupporting

confidence: 72%

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

Molnár-Láng

Molnár

Szakács

et al. 2013

Genomics of Plant Genetic Resources

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Section: Wheat × Rye Crossabilitysupporting

confidence: 72%

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

Molnár-Láng

Molnár

Szakács

et al. 2013

Genomics of Plant Genetic Resources

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“…Chromosome 5B is nearly 870 Mb (5BL, 580 Mb and 5BS, 290 Mb) and is known to contain a number of resistance genes such as Pm36 (Blanco et al 2008), Tsn1 (Faris et al 2010), Ml3D232 and PmAS846, Ph1 involved in homoeologous pairing (Sidhu et al 2008), and SKr and Kr1 related to intergeneric crossability of wheat (Alfares et al 2009). Chromosome 5B seems to have important biological functions in wheat breeding.…”

Section: Discussionmentioning

confidence: 99%

High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat (Triticum turgidum var. dicoccoides)

Xue

Wang

et al. 2012

Theor Appl Genet

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Powdery mildew, caused by Blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. The dominant powdery mildew resistance gene PmAS846 was transferred to the hexaploid wheat lines N9134 and N9738 from wild emmer wheat (Triticum dicoccoides) in 1995, and it is still one of the most effective resistance genes in China. A high resolution genetic map for PmAS846 locus was constructed using two F(2) populations and corresponding F(2:3) families developed from the crosses of N9134/Shaanyou 225 and N9738/Huixianhong. Synteny between wheat and Brachypodium distachyon and rice was used to develop closely linked molecular markers to reduce the genetic interval around PmAS846. Twenty-six expressed sequence tag-derived markers were mapped to the PmAS846 locus. Five markers co-segregated with PmAS846 in the F(2) population of N9134/Shaanyou 225. PmAS846 was physically located to wheat chromosome 5BL bin 0.75-0.76 within a gene-rich region. The markers order is conserved between wheat and Brachypodium distachyon, but rearrangements are present in rice. Two markers, BJ261635 and CJ840011 flanked PmAS846 and narrowed PmAS846 to a region that is collinear with 197 and 112 kb genomic regions on Brachypodium chromosome 4 and rice chromosome 9, respectively. The genes located on the corresponding homologous regions in Brachypodium, rice and barley could be considered for further marker saturation and identification of potential candidate genes for PmAS846. The markers co-segregating with PmAS846 provide a potential target site for positional cloning of PmAS846, and can be used for marker-assisted selection of this gene.

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“…Third, the marker Xcfb306 described in Alfares et al (2009) linked to the Kr locus on 5BS was tested to determine its linkage to Snn3.…”

Section: Snn3-b1 Regionmentioning

confidence: 99%

Marker development, saturation mapping, and high-resolution mapping of the Septoria nodorum blotch susceptibility gene Snn3-B1 in wheat

et al. 2015

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Septoria nodorum blotch (SNB), caused by Parastagonospora nodorum, is a severe foliar and glume disease on durum and common wheat. Pathogen-produced necrotrophic effectors (NEs) are the major determinants for SNB on leaves. One such NE is SnTox3, which evokes programmed cell death and leads to disease when recognized by the wheat Snn3-B1 gene. Here, we developed saturated genetic linkage maps of the Snn3-B1 region using two F2 populations derived from the SnTox3-sensitive line Sumai 3 crossed with different SnTox3-insensitive lines. Markers were identified and/or developed from various resources including previously mapped simple sequence repeats, bin-mapped expressed sequence tags, single nucleotide polymorphisms, and whole genome survey sequences. Subsequent high-resolution mapping of the Snn3-B1 locus in 5600 gametes delineated the gene to a 1.5 cM interval. Analysis of micro-colinearity of the Snn3-B1 region indicated that it was highly disrupted compared to rice and Brachypodium distachyon. The screening of a collection of durum and common wheat cultivars with tightly linked markers indicated they are not diagnostic for the presence of Snn3-B1, but can be useful for marker-assisted selection if the SnTox3 reactions of lines are first determined. Finally, we developed an ethyl methanesulfonate-induced mutant population of Sumai 3 where the screening of 408 M2 families led to the identification of 17 SnTox3-insensitive mutants. These mutants along with the markers and high-resolution map developed in this research provide a strong foundation for the map-based cloning of Snn3-B1, which will broaden our understanding of the wheat-P. nodorum system and plant-necrotrophic pathogen interactions in general.

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Fine Mapping and Marker Development for the Crossability Gene SKr on Chromosome 5BS of Hexaploid Wheat (Triticum aestivum L.)

Cited by 38 publications

References 69 publications

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat (Triticum turgidum var. dicoccoides)

Marker development, saturation mapping, and high-resolution mapping of the Septoria nodorum blotch susceptibility gene Snn3-B1 in wheat

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