Fine-mapping and validating qHTSF4.1 to increase spikelet fertility under heat stress at flowering in rice

Ye, Changrong; Tenorio, Fatima A.; Redoña, Edilberto D.; Morales–Cortezano, Portia S.; Cabrega, Gleizl A.; Jagadish, Krishna S.V.; Gregorio, Glenn B.

doi:10.1007/s00122-015-2526-9

Cited by 63 publications

(60 citation statements)

References 37 publications

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“…From the above studies and our own evaluation, it can be concluded that high‐temperature is a major factor in regulating the stability of hybrid rice production, with hybrid rice being more sensitive to increasing temperature than indica and japonica inbreds. These findings, therefore, highlight the urgent need to address the damage caused by HDT on hybrids and develop heat‐tolerant hybrids by utilizing the genetic advances made using inbreds and landraces (Ye et al, ). In Experiment 2, HDT and HNDT significantly decreased spikelet fertility in all tested genotypes, whereas HNT moderately decreased spikelet fertility in only three hybrids (Table ).…”

Section: Discussionmentioning

confidence: 99%

Pollen germination and in vivo fertilization in response to high‐temperature during flowering in hybrid and inbred rice

Shi

Schmidt

et al. 2018

Plant Cell & Environment

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High-temperature during flowering in rice causes spikelet sterility and is a major threat to rice productivity in tropical and subtropical regions, where hybrid rice development is increasingly contributing to sustain food security. However, the sensitivity of hybrids to increasing temperature and physiological responses in terms of dynamic fertilization processes is unknown. To address these questions, several promising hybrids and inbreds were exposed to control temperature and high day-time temperature (HDT) in Experiment 1, and hybrids having contrasting heat tolerance were selected for Experiment 2 for further physiological investigation under HDT and high-night-time-temperature treatments. The day-time temperature played a dominant role in determining spikelet fertility compared with the night-time temperature. HDT significantly induced spikelet sterility in tested hybrids, and hybrids had higher heat susceptibility than the high-yielding inbred varieties. Poor pollen germination was strongly associated with sterility under high-temperature. Our novel observations capturing the series of dynamic fertilization processes demonstrated that pollen tubes not reaching the viable embryo sac was the major cause for spikelet sterility under heat exposure. Our findings highlight the urgent need to improve heat tolerance in hybrids and incorporating early-morning flowering as a promising trait for mitigating HDT stress impact at flowering.

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Section: Discussionmentioning

confidence: 99%

Pollen germination and in vivo fertilization in response to high‐temperature during flowering in hybrid and inbred rice

Shi

Schmidt

et al. 2018

Plant Cell & Environment

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“…However, little is known on how the hybrid rice seed production system responds to high temperature. A number of studies have identified QTLs for heat tolerance at the flowering stage of rice, but genetically how parent plants of hybrid rice adapt to heat stress is lacking (Cao et al, 2003; Xiao et al, 2011; Ye et al, 2012, 2015). …”

Section: Introductionmentioning

confidence: 99%

High Temperature Induced Glume Closure Resulted in Lower Fertility in Hybrid Rice Seed Production

Yan

Zhang

et al. 2017

Front. Plant Sci.

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Predicted climate changes, in particular, the increased dimension and frequency of heat waves, are expected to affect crop growth in the future seriously. Hybrid rice relies on seed production from male sterile and restorer lines. Experiments were conducted over two consecutive years to compare the high temperature tolerance of parents of different hybrid rice combinations, in terms of fertility rate, flowering pattern, pollination and physiological parameters of the lodicule. Three male sterile lines and a broad compatibility restorer line (as pollen donor and conventional variety as well) were grown to heading stage and then treated with average daily temperatures of 26°C (range 23–30°C), 28°C (25–32°C), and 30°C (26–34°C), respectively, continued for 5–7 days each in a natural light phytotron which simulated the local typical high temperature weather in the field. The results indicated that male sterile lines were more sensitive to high temperature than the restorer line for fertility rate, and the sensitivity varied between varieties. The fertility rate of the restorer line was maintained at about 90% under the high temperature treatments, while it decreased in the male sterile lines by 23.3 and 48.1% at 28 and 30°C, respectively. The fertility rate of the most sensitive line declined by 70%, and the tolerant line declined by 34% at 30°C. Glume closure in the male sterile lines was a major reason for the reduced fertility rate under high temperature, which is closely correlated with carbohydrates content and the vascular bundle pattern in the lodicule. The present study identified a useful trait to select male sterile lines with high temperature tolerance for seed production.

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“…(15.1 ± 6.3%), while, in BC 5 F 2 , spikelet fertility of plants with qHTSF4.1 (44.6 ± 13.1%) was significantly higher than in plants without the QTL (27.1 ± 9.6%) and recurrent parent IR64 (19.4 ± 8.4%) (Ye et al, 2015b). n22 has many undesirable agronomic characteristics (Manigbas et al, 2014), but the results imply the importance of making best use of the landrace as a genetic resource for future breeding challenges.…”

Section: Genetic Analyses For Emf Traitmentioning

confidence: 90%

“…To fine map and validate the effect of heat tolerance QTL qHTSF4.1, PCR-based SnP markers were developed and used to genotype BC 2 F 2 , BC 3 F 2 , BC 3 F 3 , and BC 5 F 2 populations from the cross combination of IR64/n22. The QTL interval was narrowed down to about 1.2 Mb (Ye et al, 2015b). A near-isogenic line (nIL) carrying qHTSF4.1 in the IR64 background was developed.…”

Section: Genetic Analyses For Hiss At Floweringmentioning

confidence: 99%

“…In the genetic analyses using Giza178 as a donor parent, some other QTLs for heat tolerance at flowering were detected (Ye et al, 2015a), and further efforts are being made to develop the series of nILs carrying the QTLs at different loci for heat tolerance at flowering. Recent studies showed that heat tolerance at flowering stage in rice is controlled by recessive genes (Fu et al, 2015;Ye et al, 2012Ye et al, , 2015b. Among the QTLs identified for rice heat tolerance at flowering stage, even QTLs with a large effect can explain only around 20% of the variation, and the additive effect of each QTL is low (Table 1).…”

Section: Genetic Analyses For Emf Traitmentioning

confidence: 99%

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Breeding efforts to mitigate damage by heat stress to spikelet sterility and grain quality

Ishimaru

Hirabayashi

Sasaki

et al. 2016

Plant Production Science

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Global warming is predicted to aggravate the risk of unstable crop production. It is of great concern that damage to rice spikelet sterility and grain quality will increase, resulting in yield and economic losses. To secure the global food supply and farmers' income, the development of rice cultivars with heat resilience is a pressing concern. Regarding spikelet sterility, rice cultivars with heat tolerance at different growth stages have been identified in recent years. The early-morning flowering (EMF) trait is effective in heat escape because it shifts the time of day of flowering to earlier in the morning when it is cooler. Although varietal differences are very small, there are some genetic resources for EMF in wild rice accessions. Regarding heat-induced grain chalkiness, heat-tolerant Japonica-type cultivars for mitigating white-back type of chalky grains (WBCG) were found. Quantitative trait loci for heat tolerance at flowering, EMF, and for WBCG in grain quality have been mapped on the rice chromosomes. Further genetic efforts have been successfully connected to the development of near-isogenic lines for each trait with tagged molecular markers. These breeding materials are quite unique and useful in facilitating marker-assisted breeding toward the development of heat-resilient rice in terms of spikelet sterility and grain quality.

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Fine-mapping and validating qHTSF4.1 to increase spikelet fertility under heat stress at flowering in rice

Cited by 63 publications

References 37 publications

Pollen germination and in vivo fertilization in response to high‐temperature during flowering in hybrid and inbred rice

Pollen germination and in vivo fertilization in response to high‐temperature during flowering in hybrid and inbred rice

High Temperature Induced Glume Closure Resulted in Lower Fertility in Hybrid Rice Seed Production

Breeding efforts to mitigate damage by heat stress to spikelet sterility and grain quality

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