Fine mapping of a Phytophthora-resistance locus RpsGZ in soybean using genotyping-by-sequencing

Abstract

Background: Phytophthora root rot (PRR) caused by Phytophthora sojae (P. sojae) is one of the most serious limitations to soybean production worldwide. The identification of resistance gene(s) and their incorporation into elite varieties is an effective approach for breeding to prevent soybean from being harmed by this disease. A valuable mapping population of 228 F 8:11 recombinant inbred lines (RILs) derived from a cross of the resistant cultivar Guizao1 and the susceptible cultivar BRSMG68 and a high-density genetic linkage map with an average distance of 0.81 centimorgans (cM) between adjacent bin markers in this population were used to map and explore candidate gene(s). Results: PRR resistance in Guizao1 was found to be controlled by a single Mendelian locus and was finely mapped to a 367.371-kb genomic region on chromosome 3 harbouring 19 genes, including 7 disease resistance (R)-like genes, in the reference Willliams 82 genome. Quantitative real-time PCR assays of possible candidate genes revealed that Glyma.03 g05300 was likely involved in PRR resistance. Conclusions: These findings from the fine mapping of a novel Rps locus will serve as a basis for the cloning and transfer of resistance genes in soybean and the breeding of P. sojae-resistant soybean cultivars through markerassisted selection.

QTL mapping for soybean (Glycine max L.) leaf chlorophyll-content traits in a genotyped RIL population by using RAD-seq based high-density linkage map

“…These approaches mainly include resequencing, genotyping by sequencing (GBS) [15], specific length amplified fragment sequencing (SLAF-seq) [16], restriction site-associated DNA tag sequencing (RAD-seq) [17], and 2b-RAD [18]. The NGS technologies have been widely adopted in soybean, rice, wheat, sunflower and other crops [19][20][21][22][23]. Notably, QTL mapping by using soybean recombinant inbred line (RIL) populations and their RAD-seq based recombination bin marker genetic maps were reported to identify important soybean traits QTLs in recent years [24,25].…”

Identification of resistance loci toward Phytophthora sojae in South Korean soybean plant introductions 407974B and 424487B

“…Jiang et al. (2020) reported Glyma.03g035300 may be involved in defense mechanisms against P. sojae and showed significantly high expression in ‘Guizao’ ( P. sojae resistant) compared with ‘BRSMG68’ ( P. sojae susceptible). Thus, Glyma.03g035300 , or an allele of this gene in PI 407974B, is a potential candidate for silencing studies to clarify its specificity and role for P. sojae resistance toward OH7 and 1.S.1.1 in this accession.…”

Identification and molecular mapping of Rps14, a gene conferring broad-spectrum resistance to Phytophthora sojae in soybean

“…However, Rps14 region overlaps with the regions harboring Rps7, RpsUN1, Rps gene in Waseshiroge, RpsYD25, RpsYD29, and RpsGZ (Supplementary Table 10). Some of the 13 P. sojae isolates can defeat Rps7, Rps gene in Waseshiroge, and RpsUN1 (Dorrance et al 2007;Sugimoto et al 2011;Lin et al 2013), but it remains unknown whether RpsYD25, RpsYD29, and RpsGZ are resistant to these 13 isolates (Fan et al 2009;Zhang et al 2013;Jiang et al 2020). Finer mapping and further comparison of resistance spectra of these genes are needed to further determine whether Rps14 is different from these three genes.…”

“…2011; Fan et al 2009;Yao et al 2010;Wu et al 2011;Sun et al 2011;Lin et al 2013;Zhang et al 2013Zhang et al , 2014Ping et al 2016;Li et al 2016Li et al , 2017Sahoo et al 2017;Cheng et al 2017;Niu et al 2017;Zhong et al 2018Zhong et al , 2019Zhong et al , 2020Jiang et al 2020). More than half of those genes/alleles, including the Rps1 locus (five alleles, Rps1-a, , Rps7, Rps9, RpsYD25, RpsYD29, RpsUN1, Rps gene in "Waseshiroge," Rps gene in E00003, RpsQ, RpsWY, RpsHN, RpsHC18, RpsGZ and RpsX, were mapped to chromosome 3.…”