Fine mapping of the Rsv1-h gene in the soybean cultivar Suweon 97 that confers resistance to two Chinese strains of the soybean mosaic virus

Abstract

The Rsv1 - h gene in cultivar Suweon 97, which confers resistance to SMVs, was mapped to a 97.5-kb location (29,815,195-29,912,667 bp on chromosome 13) in the Rsv1 locus, thereby providing additional insights into the molecular nature underlying variations in resistance alleles in this particular locus. Soybean mosaic virus (SMV) is a well-known devastating pathogen of soybean (Glycine max (L.) Merrill.) causing significant yield losses and seed quality deterioration. A single dominant allele, Rsv1-h, which confers resistance to multiple SMV strains, was previously reported in the cultivar Suweon 97, but its exact location is unknown. In the present study, Suweon 97 was crossed with a SMV-sensitive cultivar, Williams 82. Inoculating 267 F individuals with two Chinese SMV strains (SC6-N and SC7-N) demonstrated that one single dominant gene confers SMV resistance. Another 1,150 F individuals were then screened for two simple sequence repeat (SSR) markers (BARCSOYSSR_13_1103 and BARCSOYSSR_13_1187) that flank the Rsv1 locus. Seventy-four recombinants were identified and 20 additional polymorphic SSR markers within the Rsv1 region were then employed in genotyping these recombinants. F and F recombinant lines were also inoculated with SC6-N and SC7-N to determine their phenotypes. The final data revealed that in Suweon 97, the Rsv1-h gene that confers resistance to SC6-N and SC7-N was flanked by BARCSOYSSR_13_1114 and BARCSOYSSR_13_1115, two markers that delimit a 97.5-kb region in the reference Williams 82 genome. In such region, eight genes were present, of which two, Glyma13g184800 and Glyma13g184900, encode the characteristic CC-NBS-LRR type of resistance gene and were considered potential candidates for Rsv1-h.

Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range

“…Recently, Ma et al . () mapped Rsv1‐h , which confers resistance to G1–G7, SC‐6N and SC‐7N strains, to a 97.5‐kb region and identified two CC‐NB‐LRR‐type R gene candidates in the Rsv1 chromosomal region. More detail on the Rsv1 chromosomal interval is provided in Table .…”

“…The Japanese system classifies SMV isolates into strains A, B, C, D and E, and considers both biological and sequence data (Saruta et al, 2005). The Chinese system uses several soybean differential lines to classify SMV strains SC1-21 (Li K. et al, 2010;Ma et al, 2016;Wang et al, 2003).…”

“…Yang et al (2013) mapped Rsc-ps and Rsc-pm conditioning resistance to SC3, SC6, SC7 and SC17 to the same chromosomal region. Recently, Ma et al (2016) mapped Rsv1-h, which confers resistance to G1-G7, SC-6N and SC-7N strains, to a 97.5-kb region and identified two CC-NB-LRR-type R gene candidates in the Rsv1 chromosomal region. More detail on the Rsv1 chromosomal interval is provided in Table 1.…”

Characterization of broad‐spectrum resistance to Soybean mosaic virus in soybean [Glycine max (L.) Merr.] cultivar ‘RN‐9’

“…Rsc14-r was fine mapped in a 136.8-kb genomic region containing 16 candidate genes, but none of them belongs to NBS-LRR family (Table 5). However, Rsc14Q was positioned in the vicinity of Rsv1 on chromosome 13, the region which contains a cluster of NBS-LRR genes (Hayes et al 2004;Ma et al, 2011;Ma et al, 2016;Shi et al 2008;Yang et al, 2013) (Figure 3c). Moreover, linkage analysis revealed that the Rsc10-r was mapped on chromosome 6 (Figure 3b), while that of in KF (Rsc10) was located in the vicinity of Rsv4 on chromosome 2 (Li et al, 2012) (Figure 3d), of which resistance was probably attributed to an original class of disease resistance genes (Ilut et al 2016;Maroof et al, 2010;Zhao et al 2016).…”

“…However, Rsc14Q was positioned in the vicinity of Rsv1 on chromosome 13, the region which contains a cluster of NBS‐LRR genes (Hayes et al. ; Ma et al., ; Ma et al., ; Shi et al. ; Yang et al., ) (Figure c).…”