Fine Mapping the Soybean Mosaic Virus Resistance Gene in Soybean Cultivar Heinong 84 and Development of CAPS Markers for Rapid Identification

Abstract

Heinong 84 is one of the major soybean varieties growing in Northeast China, and is resistant to the infection of all strains of soybean mosaic virus (SMV) in the region including the most prevalent strain, N3. However, the resistance gene(s) in Heinong 84 and the resistant mechanism are still elusive. In this study, genetic and next-generation sequencing (NGS)-based bulk segregation analysis (BSA) were performed to map the resistance gene using a segregation population from the cross of Heinong 84 and a susceptible cultivar to strain N3, Zhonghuang 13. Results show that the resistance of Heinong 84 is controlled by a dominant gene on chromosome 13. Further analyses suggest that the resistance gene in Heinong 84 is probably an allele of Rsv1. Finally, two pairs of single-nucleotide-polymorphism (SNP)-based primers that are tightly cosegregated with the resistance gene were designed for rapidly identifying resistant progenies in breeding via the cleaved amplified polymorphic sequence (CAPS) assay.

The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci

“…Soybean varieties Heihe 43, Dongsheng 7, Hefeng 55, ZYD00006, Dongnongdou 252, Suinong 14, Dongnongdou 254, Heinong 84, Zhonghuang 13, and Dongnong 50 were grown in pot in a growth chamber with 50% humidity and 16:8 (light: dark) photoperiod at 26 • C. The hybrid population from the crossing between Heinong 84 and Zhonghuang 13 has been reported previously [22].…”

“…Previously, we constructed a soybean hybrid population by crossing Heinong 84 and Zhonghuang 13 to locate the resistance gene against the soybean mosaic virus strain N3 in Heinong 84 [22]. We thus decided to take advantage of this hybrid population to analyze the genetic basis of the trait of ALSV resistance of Heinong 84.…”

“…Sequences (5 ′ -3 ′ ) Usage Total genome DNA was isolated using the FastPure Plant DNA Isolation Mini Kit (Vazyme Biotech). DNA pools were prepared as described earlier with few modifications [22]. The susceptible and resistant pools included 30 susceptible and resistant soybean DNA of 6 µg DNA per sample, respectively.…”

P1 of turnip mosaic virus interacts with NOD19 for vigorous infection

“…In brief, Agrobacterium strain GV3101 harboring the proper plasmid were washed with infiltration buffer (10 mM MES, pH 5.6, 10 mM MgCl; 100 μM acetosyringone) twice by low-speed centrifuge at room temperature and infiltrated into N. benthamiana leaves at the indicated OD values. Mechanical inoculation was performed as described earlier (Li et al, 2022). The whole aerial part was taken for further analyses, e.g., RNA extraction and Western blot.…”

WGCNA Reveals Hub Genes and Key Gene Regulatory Pathways of the Response of Soybean to Infection by Soybean mosaic virus

“…Based on the interaction between viruses and plants, the types of plant resistance to viruses are thought to include dominant resistance and recessive resistance [14,15]. To our knowledge, approximately 34 single dominant resistance loci for SMV located on six chromosomes have been identified and reported in soybean: chromosome 02 (Rsv4, RSC5, RSC6, RSC7, RSC8, RSC10, RSC17, RSC18A, RSC3(w), RkSC13, Rsc13, RSC9, and Rsc3k) [16][17][18][19][20][21], chromosome 03 (qTsmv-13) [22], chromosome 06 (RSC15, RSC18) [2,20], chromosome 11(RSMV-11) [23], chromosome 13 (Rsv1, Rsv5, RSC3Q, RSC11, RSC12, RSC14Q, RSC18B, RSC20, RSC-pm, RSC-ps, Rsv1-r, RSC15ZH, qSMV13, and Rsvg2) [20,[24][25][26][27][28], and chromosome 14 (Rsv3, RSC4, and rySC13) [20,29]. Among the above five resistance loci, for which gene cloning and functional analysis have been completed, Glyma.14g204700 on Rsv3 [30], Rsc4-3 on Rsc4 [31,32], GmCAL on Rsc8 [33], GmST1 on Rsvg2 [28], and a recombinant gene on Rsc3k are involved in soybean resistance to SMV [17].…”