Fine structural features of light and dark cells in the trigeminal ganglion of the rat

“…The large pale neurons in the normal trigeminal ganglia or the ganglia of control rats injected with saline varied in size but were usually much larger than the darkly stained neurons (Peach, 1972). Their nuclei and nucleoli were larger than the corresponding structures in types B and C neurons.…”

Section: Resultsmentioning

confidence: 98%

A method for peripheral chromatolysis in neurons of trigeminal and dorsal root ganglia, produced in rats by lithium

Levine

Saltzman

Kumar

2004

Journal of Neuroscience Methods

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“…Published electron microscopy (EM) investigations of the cellular organelles in sensory neuron somata do not reveal presence of vesicles in proximity to the plasma membrane of the type that are commonly observed at the synaptic terminal specializations of these neurons in the sympathetic ganglia (Heym et al, 1993), the spinal cord or brainstem (Knyihar-Csillik et al, 1982;Carlton and Hayes 1989;Plenderleith et al, 1990;Valtschanoff et al, 1994), or at the reconstructed peripheral axon terminals of unmyelinated sensory neurons (Kruger et al, 2003). Only one study illustrates a single vesicle in close apposition to the somatic plasma membrane of IB4 (+) neurons (Streit et al, 1986), whereas only scattered cytoplasmic vesicles may be found at considerable distances from the plasma membrane in others (Dixon 1963;Pineda et al, 1967;Peach 1972;Lehtosalo et al, 1984). What can account for such a discrepancy between physiological and anatomical studies?…”

Section: Anatomical Evidence Of Vesicular Releasementioning

confidence: 99%

Two types of neurotransmitter release patterns in isolectin B4-positive and negative trigeminal ganglion neurons

et al. 2007

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Mammalian nociceptors have been classified into subclasses based on differential neurotrophin sensitivity and binding of the plant isolectin B4 (IB4). Most of the nerve growth factor-responsive IB4-negative (IB4 (-)) nociceptors contain neuropeptides such as substance P and calcitonin gene-related peptide, whereas the glial-derived neurotrophic factor-responsive IB4-positive (IB4 (+)) neurons predominantly lack such neuropeptides. We hypothesized that the differences in neuropeptide content between IB4 (+) and (-) neurons might be reflected in differences in stimulated exocytosis and/or endocytosis. To address this, we monitored the secretory activity of acutely dissociated neurons from adult rat trigeminal ganglia (TRG) using cell membrane capacitance (Cm) measurements and the fluorescent membrane-uptake marker N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide (FM4-64). Cm measurements were performed under whole-cell voltage clamp and neurons were depolarized from -75 mV to +10 mV to elicit exocytosis. Both types of TRG neurons showed similarly-sized, calcium-dependent increases in Cm, demonstrating that both IB4 (+) and (-) TRG neurons are capable of stimulated exocytosis. However, the peak Cm of IB4 (+) neurons decayed faster toward baseline than that of IB4 (-) neurons. Also, IB4 (+) neurons had stable Cm responses to repeated stimuli whereas IB4 (-) neurons loss their secretory response during repeated stimulation. These data suggested that the IB4 (+) neurons possess a faster rate of endocytosis and vesicle replenishment than IB4 (-) neurons. To test this, we measured vesicle trafficking with the fluorescent membrane dye FM4-64. FM4-64 staining showed that IB4 (-) neurons exhibit a larger pool of endocytosed vesicles than IB4 (+) neurons because the peak fluorescence increases in IB4 (-) neurons were larger but slower than in IB4 (+) neurons. However, the recycled vesicles were released faster in IB4 (+) compared with IB4 (-) neurons. Taken together these data suggest that the IB4 (+) TRG neurons have faster exocytosis and endocytosis than the IB4 (-) neurons.

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“…The significance of dark and pale neurons in the central nervous system has been a controversial matter even at the ultrastructural level (DAWSON, HOSSACK and WYBURN, 1955;ROSENBLUTH, 1962;DIXON, 1963;GONATAS, 1966;PEACH, 1969;JAIM ETCHEVERRY and PELLEGRINO DE IRALDI, 1968;SUBURO and PELLEGRINO DE IRALDI, 1969). At least three possible explanations have been brought forward concerning the appearance of dark and light cells in the central nervous system, i.e., fixation artefacts (ROSENBLUTH, 1962;GONATAS, 1966), cell malnutrition (ROSENBLUTH and PALAY, 1961), and different levels of physiological activity (ZAMBRANO and DE ROBERTIS, 1968).…”

Section: Discussionmentioning

confidence: 99%

Fine Structure of Neurons in the Spinal Ganglion of Slow Loris (<i>Nycticebus coucang coucang</i>)

Ahmed

1973

Arch. Histol. Jap., Arch. Histol. Jpn

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Fine structural features of light and dark cells in the trigeminal ganglion of the rat

Cited by 65 publications

References 18 publications

A method for peripheral chromatolysis in neurons of trigeminal and dorsal root ganglia, produced in rats by lithium

A method for peripheral chromatolysis in neurons of trigeminal and dorsal root ganglia, produced in rats by lithium

Two types of neurotransmitter release patterns in isolectin B4-positive and negative trigeminal ganglion neurons

Fine Structure of Neurons in the Spinal Ganglion of Slow Loris (<i>Nycticebus coucang coucang</i>)

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