Fine Structure and Cytochemistry of Human Prostatic Carcinoma

Kirchheim, D; Brandes, David; Bacon, Rebecca

doi:10.1016/b978-0-12-125650-0.50021-1

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…The failure of histochemical zinc demonstration also reflects this loss of functional activity. Similar observa tions have been made in human prostatic car cinoma (24,37).…”

Section: Discussionsupporting

confidence: 64%

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

Tunn¹,

Senge²,

Schenck³

et al. 1980

Urol Int

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The effect of 3α-androstanediol (3α-diol), 17β-estradiol (E₂) and cyproterone acetate (CA) on prostates in castrated beagle dogs were investigated by histologjcal and histochemical examinations. A significant increase of prostatic weight occurred after 6 months’ treatment with 3α-diol alone and in combination with E₂. Histologically and histochemically, two different types of prostatic enlargement were observed: first, administration of 3α-diol resulted in diffuse glandular hyperplasia with replacement of functional activity monitored by strongly positive reactions for acid phosphatase, aminopeptidase and zinc. Second, 3α-diol plus E₂ produced stratified squamous metaplasia with cystic lumina and loss of the typical morphological structure. These glands showed negative reactions for acid phosphatase, aminopeptidase and zinc. In both types of prostatic hyperplasia CA abolished epithelial or metaplastic proliferation and induced atrophy of glandular epithelium. In estrogenized dogs activation of the fibromuscular stroma was obvious. CA prevented prostatic hyperplasia by atrophying epithelial effects.

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“…The failure of histochemical zinc demonstration also reflects this loss of functional activity. Similar observa tions have been made in human prostatic car cinoma (24,37).…”

Section: Discussionsupporting

confidence: 64%

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

Tunn¹,

Senge²,

Schenck³

et al. 1980

Urol Int

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“…It is now shown ultrastructurally that the darkening of dysplastic cytoplasm represents the loss of myriad secretory vacuoles which nearly fill the normal cell. Their generation and secretory discharge probably constitute the most active differentiated function of the normal prostate cell [7,8]. Their disappearance, along with the depletion of differentiated proteins such as PSA and PAP, clearly characterized dysplasia as a disease of impaired cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

The prostatic epithelial cell in dysplasia: An ultrastructural perspective

deVries¹,

McNeal²,

Bensch³

1992

The Prostate

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The histologic features of prostatic duct-acinar dysplasia have been difficult to analyze ultrastructurally, because of the difficulty in properly selecting and processing such small, randomly situated grossly invisible lesions. We have succeeded in identifying dysplastic foci by examination of the cut surfaces of tissue slices under low magnification. Dysplasia foci were excised from the slices and were compared to adjacent normal tissue by both light and electron microscopy. By electron microscopy (EM), normal secretory cells were filled with myriad tiny clear vacuoles, which were markedly diminished to absent in the cytoplasm of dysplastic cells. Both apocrine and eccrine secretion characterized normal epithelium and were diminished in dysplasia. EM showed striking features of nuclear abnormality more prominently than light microscopy, and qualitative basement membrane abnormalities were revealed. By EM analysis, dysplastic epithelium resembled that of invasive carcinoma more than normal epithelial cells.

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“…Further studies should be conducted to determine if blebbing is related to the age of the animals, with older animals yielding cultures with a higher incidence of blebs. Kirchheim et al (1974) have reported that intracellular lumens develop in vivo in epithelium from human prostatic carcinomas. The intracellular blebs seen in the cultures may be a related phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Primary epithelial cell cultures derived from canine prostate: Isolation, culture, and characterization

et al. 1982

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Epithelial-cell-enriched primary cultures were established from canine prostate. Minced tissue was dissociated with 750 units/ml of collagenase in F12K tissue culture medium containing 1% fetal bovine serum. This treatment resulted in the gradual removal of stromal elements from the base of the epithelial cells. After 30 minutes of digestion, aggregates of epithelial cells free of stroma were dislodged from the minced pieces of prostate. These aggregates were washed and plated at high density in F12K plus 10% fetal bovine serum. After 12-16 hours in vitro the unattached cellular aggregates were removed from the culture dishes, washed, and reinoculated into new culture vessels containing fresh medium. After 48 hours in vitro, the aggregates had attached to the culture vessels and spread out to yield discrete patches of epithelial cells. By 120 hours in vitro the patches of cells had grown and coalesced to form a confluent monolayer of epithelial cells. Ultrastructural examination of these cultures indicated that adjacent cells were joined by desmosomes and tight junctions and had tonofilaments and microvilli, giving the cells an epithelial appearance. The cells contained rough endoplasmic reticulum, Golgi apparatus, and secretory granules similar to those of the epithelial cells in the intact organ. In addition, intracellular "blebs" containing acid phosphatase were observed in the monolayers and were found to increase in size and number with time in vitro. Differentiated function of the cultures was demonstrated by the presence of ornithine decarboxylase and acid phosphatase and the ability of the cultures to metabolize testosterone to primarily 5 alpha-reduced metabolites.

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Fine Structure and Cytochemistry of Human Prostatic Carcinoma

Cited by 12 publications

References 21 publications

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

The prostatic epithelial cell in dysplasia: An ultrastructural perspective

Primary epithelial cell cultures derived from canine prostate: Isolation, culture, and characterization

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