Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins

Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

“…In immunoelectron microscopy (IEM) the incubation of ultrathin cryostat sections with anti-p 17 and anti-p24 MAbs led to a labelling of internal viral components (Fig. 2 a, b, c) thus corroborating earlier observations on the localization of the structural proteins (Gelderblom et al, 1987(Gelderblom et al, , 1988. In pre-embedding IEM, a technique allowing the exclusive detection of external antigens, no labelling was observed with these antibodies (data not shown).…”

Human retroviruses and AIDS 1997

“…From cross-linking and molecular-genetic experiments both trimer-and dimerformation have been reported . From morphologic studies a trimeric shape of the knobs became evident (Gelderblom et al, 1987a;Gelderblom et al, 1988;Grief etal., 1989).…”

“…Constituents and organization of the viral core While MA in the mature virion remains tightly bound to the inner leaflet of the lipid-bilayer, the other Gag-proteins condense to form the cone-shaped core typical of members of the lentivirus family (Figs. 4-7;Gelderblom et al, 1987a). In addition, cleavage of Prl55 Gag-Pol liberates multiple copies of the three essential viral enzymes: reverse transcriptase (RT), integrase (IN), and protease (PR).…”

“…The HTV Env complex has been described as a tettamer and as a dimer of dimers Pinter et al, 1989; as well as a trimer (Gelderblom et al, 1987;Weiss etal., 1990). The trimeric nature of the gp41 coiled-coil complex, even though it is formed in the absence of gpl20, clearly suggests that the native Env complex is also trimeric.…”

“…This process can be inhibited by the intercalation of short peptides derived from the respective domains (Niedrig et al, 1993). The core capsid is made up of p24 CA (Gelderblom et al, 1987a;Chrystie and Almeida, 1989). Domains mainly at the C-terminus of p24 CA are essential for proper assembly of the RNP shell , while the N-terminal region is required for the formation of the mature, cone-shaped core.…”