Fine structure of the honeybee Z-disc

Saide, Judith D.; Ullrick, William C.

doi:10.1016/0022-2836(73)90009-0

Cited by 26 publications

(19 citation statements)

References 9 publications

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“…The oblique sections we have examined enter the myofibril above the Z disk, pass through it, and exit below it. Threedimensional information can be obtained by studying the change in projected density across the section, which corresponds to different levels of sectioning along the myofibril axis (Ashhurst, 1977;Saide and Ullrick, 1973). The oblique section in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…There has been considerable uncertainty about the structure of the insect flight muscle Z disk. Its fine structure has been investigated in Calliphora, Lethocerus, and honeybee (Auber and Couteaux, 1963;Saide and Ullrick, 1973;Ashhurst, 1967a,b;reviewed by Ashhurst, 1977). The structures that have been proposed differ in many significant molecular details, including whether either (or both) thick and thin illaments (or extensions from their ends) participate in the Z disk network, and whether actin filaments enter and overlap inside the Z disk or terminate at its borders.…”

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confidence: 99%

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Arrangement of filaments and cross-links in the bee flight muscle Z disk by image analysis of oblique sections.

Deatherage

Cheng

Bullard

1989

The Journal of Cell Biology

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Abstract. Information from oblique thin sections and from three-dimensional reconstructions of tilted, transverse thin sections (Cheng, N., and J. E Deatherage.1989. J. Cell Biol. 108:1761-1774) has been combined to determine the three-dimensional structure of the honeybee flight muscle Z disk at 70-/~ resolution. The overall symmetry and structure of the Z disk and its relationship to the rest of the myofibril have been determined by tracing filaments and connecting elements on electron images of oblique sections which have been enhanced by a local crystallographic averaging technique. In the three-dimensional structure, the connecting density between actin filaments can be described as five compact, crystallographically nonequivalent domains. Features C1 and C2 are located on the transverse twofold rotation axes in the central plane of the Z disk. They are associated with the sides of actin filaments of opposite polarity. Features C3, C4, and C5 are present in two symmetry-related sets which are located on opposite sides of the central plane. C3 and C5 are each associated with two illaments of opposite polarity, interacting with the side of one filament and the end of the other filament. C3 and C5 may be involved in stabilizing actin filament ends inside the Z disk. The location of the threefold symmetric connection C4, relative to the thick filament of the adjacent sarcomere, is determined and its possible relationship to the C filament is considered.T HE Z disk of striated muscle cross-links and terminates thin filaments from adjacent sarcomeres. The Z disk of insect flight muscle, which is arranged on a hexagonal lattice, is structurally different from that of vertebrate muscle, which is arranged on a square lattice (Knappeis and Carlsen, 1962;Reedy, 1964). Although its hexagonal lattice is better ordered than the vertebrate muscle lattice, the insect flight muscle Z disk is a dense, low contrast specimen for electron microscopy.There has been considerable uncertainty about the structure of the insect flight muscle Z disk. Its fine structure has been investigated in Calliphora, Lethocerus, and honeybee (Auber and Couteaux, 1963;Saide and Ullrick, 1973; Ashhurst, 1967a,b; reviewed by Ashhurst, 1977). The structures that have been proposed differ in many significant molecular details, including whether either (or both) thick and thin illaments (or extensions from their ends) participate in the Z disk network, and whether actin filaments enter and overlap inside the Z disk or terminate at its borders. Also unsettled are the nature of the cross-links (connecting filaments, tubes, or amorphous material) and the symmetries of the components of the network.In this study we combine information from locally averaged oblique sections and three-dimensional reconstruction (Cheng and Deatherage, 1989) to construct a complete model for the honeybee Z disk. Materials and Methods Electron Microscopy and Image Processing of Oblique SectionsThin sections of freshly dissected honeybee dorsal longitudinal indirect flight musc...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Arrangement of filaments and cross-links in the bee flight muscle Z disk by image analysis of oblique sections.

Deatherage

Cheng

Bullard

1989

The Journal of Cell Biology

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“…The structural variations that are seen among invertebrate Z bands can be correlated with functional specializations of muscles. The very fast flight muscles have highly ordered Z bands that show a well-defined, regular structure (Auber & Couteaux, 1963;Ashhurst, 1967b;Saide & Ullrick, 1973). In cross-sections, these Z bands show an hexagonal lattice rather than the square lattice typical of vertebrate muscle (Ashhurst, 1967b).…”

Section: Invertebrate Musclementioning

confidence: 99%

The muscle Z band: lessons in stress management

Vigoreaux

1994

J Muscle Res Cell Motil

105

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Two of the most characteristic features of striated muscle are (i) its ability to contract and generate tension when activated and (ii) its ability to return to its original length and form after contraction or stretching ceases. These two properties are to a large extent the primary manifestations of separate sets of filament systems: contractile actin and myosin filaments and viscoelastic titin and intermediate filaments. Z bands function as a common link that mechanically integrates contractile and elastic elements and as such they play a fundamental role in transmission of active and passive forces. Differences in Z band structure have been described for distinct classes of muscle and fibre types. The diversity in Z band architecture has been built around its phylogenetically conserved role as an actin-anchoring structure. Novel proteins are likely to account for structural and functional differences seen across the phyla.

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“…There is no firm evidence to suggest that either phosphorylase or creatine kinase actually constitutes the M-line. Other studies (Landon and Oriol, 1975;Masaki and Takaiti, 19740 have indicated that the M-line contains a protein with a subunit molecular weight of 160,000 daltons, but additional evidence is needed to conclusively establish this protein as Kelly, 1967;Kelly and Cahill, 1972;Knappeis and Carlsen, 1962;Landon, 1970aLandon, , 1970bReedy, 196^;Saide and Ullrick, 1973), but none of the models emerging from these studies can completely account for the observed properties of this structure. Although there is much dis crepancy regarding Z=disk structure, all models agree that the thin filaments form a square lattice as they approach the Z-disk (Franzini-Armstrong, 1973).…”

Section: +mentioning

confidence: 99%

Purification and some properties of a Ca2+-activated muscle enzyme that removes Z-disks

Dayton

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Fine structure of the honeybee Z-disc

Cited by 26 publications

References 9 publications

Arrangement of filaments and cross-links in the bee flight muscle Z disk by image analysis of oblique sections.

Arrangement of filaments and cross-links in the bee flight muscle Z disk by image analysis of oblique sections.

The muscle Z band: lessons in stress management

Purification and some properties of a Ca2+-activated muscle enzyme that removes Z-disks

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