Fine structure of the outer enamel epithelium in the cervical loop of the rat incisor.

Abstract

IntroductionThere have been only a few morphological studies on the cervical loop (CL) of the enamel organ. It was originally reported on the basis of light microscopic observations that the CL of the rat incisor was difficult to discriminate into the four cell layers, i.e., the inner enamel epithelium, the stellate reticulum, the stratum intermedium and the outer enamel epithelium [1]. Later, however, it was shown by WARSHAWSKY et al. [2] that in the CL of the rat incisor, both the inner and outer enamel epithelia consisted of multilayers.Previous electron microscopic studies have focused mainly on the morphological changes occurring in the process of inner enamel epithelial differentiation into secretory ameloblasts [3][4][5][6][7][8][9][10][11]. Only a few investigations have been made on the outer enamel epithelium using electron microscopy; PANNESE [12] [16] all briefly reported the fine structure of the outer enamel epithelium. These studies showed that the outer enamel epithelium is lined by an outer basal lamina, and that the cells in the outer enamel epithelium have microvilli on their lateral membranes and are connected with adjacent cells by desmosomes. However, no ultrastructural details of the outer enamel epithelium have yet been clarified. It was also shown by DECKER[3] that lipid granules are often seen within cells, while details of their distrubution in the CL have not been revealed. The author therefore studied these various points in detail using the growing end of the rat incisor, and observed, mainly by electron microscopy, the fine structure of the outer enamel epithelium in the CL during the process of tooth formation.

Single-Cell RNA-Sequencing From Mouse Incisor Reveals Dental Epithelial Cell-Type Specific Genes

“…In IEE/OEE clusters, GO terms were mostly enriched in cell division and cell cycle, in agreement with their high proliferation activity ( Figure 2E ). In SI/SR clusters, GO enrichment indicated that they act as regulators of nutrient and ion transport ( Figure 2F ), in line with previous reports ( Kallenbach, 1978 ; Inage et al, 1987 ; Yoshida et al, 1989 ; Nakamura and Ozawa, 1997 ).…”