Fine structure of the taste bud in guinea pigs. II. Localization of spot 35 protein, a cerebellar Purkinje cell-specific protein, as revealed by electron-microscopic immunocytochemistry.

Souma, Yoshie; Wakasugi, Chikashi; Teraki, Yoshimi; Iwanaga, Toshihiko; Fujita, Tsuneo

doi:10.1679/aohc.54.113

Cited by 12 publications

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“…To characterize the nature of the villin-immunoreactive cells, calbindin and serotonin immunostainings were performed. Spot 35 protein (=calbindin) has been detected in type III or receptor cells of guinea pig circumvallate taste buds (Yoshie et al 1991) and also in certain cells, probably receptive in function, of bullfrog taste organs (Kuramoto 1988). Incidentally, this protein is a calciumbinding soluble protein originally isolated from the cerebellar Purkinje cell (Yamakuni et al 1984;Hozumi et al 1986).…”

Section: Resultsmentioning

confidence: 99%

“…Frog taste receptor cells, ultrastructurally identified by synapsing onto intragemmal nerve terminals, possess a discrete contour, which markedly resembles the profile of the villin-immunoreactive cells. Moreover, they showed immunoreactivity for calbindin that has been used as a marker of mammalian taste receptor cells (Yoshie et al 1991). Hence the villin-immunostained cells most probably function as taste receptors.…”

Section: Discussionmentioning

confidence: 97%

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Villin is a possible marker of receptor cells in frog taste organs

Souma

Kumakura

Toyoshima

2003

Histochemistry and Cell Biology

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We investigated lingual taste organs of four frog species mainly by means of fluorescence immunohistochemistry for villin, calbindin, and serotonin. Cells immunoreactive for villin appeared in the taste organs of all the species used. These villin-immunostained cells were basoapically elongated in shape and extended up to the apical surface. They were also immunoreactive for calbindin. On the other hand, serotonin-immunoreactive cells, identified as Merkel-like basal cells, were immunonegative for villin. Considering the present results combined with those of studies by other research groups, the villin-immunostained cells were postulated to function as taste receptors.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Villin is a possible marker of receptor cells in frog taste organs

Souma

Kumakura

Toyoshima

2003

Histochemistry and Cell Biology

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“…Fujita et al (1988) and Iwanaga et al (1992) showed that taste receptor cells are typical sensory paraneurons that their structure, metabolism, and functional characteristics are very similar to those of neurons, and that several neuron-specific proteins, such as spot 35 protein (a protein found in cerebellar Purkinje cells), NSE, and PGP 9.5, are found on certain sensory paraneurons. Yoshie et al (1991) demonstrated the fine structure of the guinea pig vallate taste bud using spot 35 protein immunoelectron microscopy and reported that the immunoreactivity was localized in the cytoplasm of type III cells. Iwanaga et al (1992), using light microscopy, reported that taste cells in rat vallate taste buds were selectively immunostained by PGP 9.5 antiserum and presumed that these were type III cells; however, this assumption was simply based on the knowledge that PGP 9.5 is a marker of certain paraneurons.…”

Section: Discussionmentioning

confidence: 99%

“…Taste receptor cells, which are typical sensory paraneurons, express certain neuron-specific proteins (Huang and Lu, 1996b;Iwanaga et al, 1992;Kanazawa and Yoshie, 1996;Yee et al, 2001). Yoshie et al (1991) demonstrated that certain guinea pig taste bud cells are immunoreactive for spot 35 protein, a protein found in cerebellar Purkinje cells, and that the ultrastructural features of these cells resemble those of type III cells and gave the evidence that paraneurons might share neuron-specific substances with neurons. Iwanaga et al (1992) and Kudoh (1996) reported that type III taste cells in rats or mice, respectively, are immunoreactive for neuron-specific enolase (NSE) or protein gene product 9.5 (PGP 9.5).…”

Section: Introductionmentioning

confidence: 98%

Immunoelectron microscopic studies on protein gene product 9.5 and calcitonin gene‐related peptide in vallate taste cells and related nerves in the guinea pig

Huang

2003

Microscopy Res & Technique

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On the basis of our previous report that protein gene product 9.5 (PGP 9.5)-immunoreactive nerve fibers and taste cells and calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers are found in guinea pig vallate papillae [Huang and Lu (1996b) Arch. Histol. Cytol. 59:433-441]. We speculated that PGP 9.5 might be a marker for taste receptor cells and that CGRP might play an important role in taste transmission. We, therefore, performed an immunohistochemical and ultrastructural analysis of taste cells and related nerves in guinea pig vallate papillae. In the connective tissue of the vallate papilla, the ultrastructural data revealed that the PGP 9.5-immunoreactive nerve fibers were both myelinated and unmyelinated. The CGRP-immunoreactive nerve fibers were unmyelinated and surrounded by the cytoplasm of Schwann cells as were the non-immunoreactive fibers. In the vallate taste buds, only type III cells, which make synaptic contacts with intragemmal nerves, were PGP 9.5-immunoreactive, while the nerve terminals making synaptic contact with the underlying type III cells were CGRP-immunoreactive. From these observations, we conclude that: (1) PGP 9.5 might be a useful specific marker for type III cells in guinea pig vallate taste buds; and (2) CGRP-containing nerve fibers might be primarily involved in the neural transmission of taste stimuli.

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“…It has been reported that type-III cells make synaptic contacts with gustatory afferents. Contradictory results regarding the types of cells demonstrating CB-LI have been reported; Johnson et al (1992) reported that they could not observe synaptic contacts with CB-IR cells in rat CVP, while Yoshie et al (1988Yoshie et al ( , 1989Yoshie et al ( , 1991 identified synaptic contacts on cells that were positive for spot 35-protein, a cerebellar Purkinje cell-specific protein (Yamakuni et al 1984) identical to CB (Lomri et al 1989), in guinea pig CVP.…”

Section: Introductionmentioning

confidence: 98%

Calbindin D28k-like immunoreactivity in the developing and regenerating circumvallate papilla of the rat

Miyawaki

Morisaki

Tabata

et al. 1997

Cell and Tissue Research

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The distribution of calbindin D28k (CB)-like immunoreactivity (-LI) in the circumvallate papilla (CVP) was examined during development and regeneration following bilateral crush injury to the glossopharyngeal nerve in the rat. In the adult CVP, CB-like immunoreactive (-IR) nerve fibers were observed in the subgemmal region and some penetrated into the taste buds. CB-LI was also detected in the cytoplasm of the spindle-shaped gustatory cells in the lower half of the trench epithelium, which contained numerous synaptic vesicles and bundles of intermediate filaments. These CB-IR gustatory cells made synapse-like contacts with CB-IR nerve terminals. Some CB-IR nerve terminals made contacts with the gustatory cells negative for CB-LI. At least three developmental stages were defined with regard to the developmental changes in the distribution of CB-LI: (1) Stage I (embryonic day (E) 18-postnatal day (P)5): CB-IR nerve fibers appeared in the lamina propria just beneath the newly-formed CVP at E18, but the gustatory epithelium of the CVP contained no CB-IR structures. Taste buds with taste pores appeared at P1. (2) Stage II (P5-10): thin CB-IR nerve fibers began entering the trench epithelium, but no CB-IR cells were observed. (3) Stage III (P10-adult): in addition to the intragemmal and perigemmal CB-IR nerve fibers, very few CB-IR cells appeared in the taste buds around P10, and their numbers increased progressively. The changes in the distribution of taste buds and CB-LI following glossopharyngeal nerve injury were similar to those observed during development. On post-operative day (PO) 4, the taste buds and CB-IR cells decreased markedly in number. These CB-IR cells became round in shape, and the number of CB-IR nerve fibers decreased markedly. On PO8, both taste buds and CB-IR cells disappeared completely. The regenerated taste buds were first observed on PO12, increased rapidly in number by PO20, and increased slowly thereafter. CB-IR nerve fibers accumulated at the subgemmal region and began penetrating into the trench wall epithelium around PO16. CB-IR cells appeared between PO20 and PO24, and their numbers increased progressively and reached the normal level on PO40. The topographical localizations of the taste buds and CB-IR cells during development and regeneration were comparable to those of normal animals. The delay of the time courses for appearance of CB-IR nerve fibers and CB-IR cells compared to the appearance of taste buds during development and regeneration suggests that CB in the gustatory epithelium may participate in the survival of the taste bud cells rather than in the induction of the taste buds.

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Fine structure of the taste bud in guinea pigs. II. Localization of spot 35 protein, a cerebellar Purkinje cell-specific protein, as revealed by electron-microscopic immunocytochemistry.

Cited by 12 publications

References 0 publications

Villin is a possible marker of receptor cells in frog taste organs

Villin is a possible marker of receptor cells in frog taste organs

Immunoelectron microscopic studies on protein gene product 9.5 and calcitonin gene‐related peptide in vallate taste cells and related nerves in the guinea pig

Calbindin D28k-like immunoreactivity in the developing and regenerating circumvallate papilla of the rat

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