FINGERPRINT ANALYSIS OF UNFRACTIONATED Piper PLANT EXTRACTS BY HPLC-UV-DAD COUPLED WITH CHEMOMETRIC METHODS

Bernal, Freddy A.; Delgado, Wilman; Cuca, Luis E.

doi:10.4067/s0717-97072012000300013

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…Hence, acquisition of UV spectra has potential advantages, even though it has not been exploited in phytoplankton chemotaxonomy. This methodology has been applied to plant chemotaxonomy [ 57 ] but most of the time, UV detection is coupled with other detectors like mass spectrometry or ELSD [ 58 ] to cluster different taxa. The field needs innovative breakthroughs to identify next generation of markers.…”

Section: Discussionmentioning

confidence: 99%

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

et al. 2017

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Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature. Using an optimized extraction process coupled to a HPLC de-replication strategy, we examined the pigment composition of 37 microalgae strains, representative of the broad taxonomic diversity of marine and freshwater species (excluding cyanobacteria). For each species, the major pigments already described were unambiguously identified. We also observed the presence of several minor unidentified pigments in each chromatogram. The global analysis of pigment compositions revealed a total of 124 pigments, including 98 pigments or derivatives unidentified using the standards. Absorption spectra indicated that 35 corresponded to chlorophyll/porphyrin derivatives, 57 to carotenoids and six to derivatives having both spectral signatures. Sixty-one of these unidentified or new carotenoids and porphyrin derivatives were characteristic of particular strains or species, indicating their possible use as highly specific chemotaxonomic markers capable of identifying one strain out of the 37 selected. We developed a graphical analysis using Gephi software to give a clear representation of pigment communities among the various phytoplankton strains, and to reveal strain-characteristic and shared pigments. This made it possible to reconstruct the taxonomic evolution of microalgae classes, on the basis of the conservation, loss, and/or appearance of pigments.

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Section: Discussionmentioning

confidence: 99%

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

et al. 2017

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“…It is one of the most diverse lineages among basal angiosperms. 1,2 Piper species have received much attention due to the potent activities and chemical diversity of their secondary metabolites, and several species have high economic value, such as P. nigrum (black pepper), P. methysticum (kava), and P. betle (betel nuts) P. divaricatum. [3][4][5] In particular, Piper arboreum, a shrub that typically grows to 3 m height, popularly known as long pepper, rosemary-of-Angola or wood-of-Angola, has been used in traditional Brazilian medicine in the form of tea for treatment of rheumatism, bronchitis, colds and flu.…”

Section: Introductionmentioning

confidence: 99%

CHEMICAL STUDY AND ANTIMICROBIAL ACTIVITIES OF Piper arboreum (PIPERACEAE)

Nascimento

Araújo

Silva

et al. 2015

J. Chil. Chem. Soc.

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This study evaluated the antifungal and antibacterial activities of the extracts and their fractions, essential oils, and major constituents of Piper arboreum tissues. The chemical compositions of P. arborem tissues and major constituent were identified using GC/MS, 1 H and 13 C NMR. All the botanic material tested proved to be specific bactericidal agents against Staphylococcus aureus, with MBC/MIC = 2, including the amide piperetine, the major compound isolated of P. arboreum for the first time. The essential oil of leaves showed antifungal activity against Microsporum gypseum and Epidermophyton floccosum, with MFC values of 312.5 and 125 µg/mL, respectively.

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“…By which the multivariate data from the complexity of herbal drugs can be analyzed by professional software to yield more objective, and reliable to elucidate the relationship of the thermal treatment with the constituents change. And similar attempts have also been applied successfully in some cases of other herbal medicines .…”

Section: Introductionmentioning

confidence: 95%

Quality assessment of crude and processed ginger by high‐performance liquid chromatography with diode array detection and mass spectrometry combined with chemometrics

Deng

Yu²,

Zhao

et al. 2015

J of Separation Science

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A sensitive, simple, and validated high-performance liquid chromatography with diode array detection and mass spectrometry detection method was developed for three ginger-based traditional Chinese herbal drugs, Zingiberis Rhizoma, Zingiberis Rhizome Preparatum, and Zingiberis Rhizome Carbonisata. Chemometrics methods, such as principal component analysis, hierarchical cluster analysis, and analysis of variance, were also employed in the data analysis. The results clearly revealed significant differences among Zingiberis Rhizoma, Zingiberis Rhizome Preparatum, and Zingiberis Rhizome Carbonisata, indicating variations in their chemical compositions during the processing, which may elucidate the relationship of the thermal treatment with the change of the constituents and interpret their different clinical uses. Furthermore, the sample consistency of Zingiberis Rhizoma, Zingiberis Rhizome Preparatum, and Zingiberis Rhizome Carbonisata can also be visualized by high-performance liquid chromatography with diode array detection and mass spectrometry analysis followed by principal component analysis/hierarchical cluster analysis. The comprehensive strategy of liquid chromatography with mass spectrometry analysis coupled with chemometrics should be useful in quality assurance for ginger-based herbal drugs and other herbal medicines.

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FINGERPRINT ANALYSIS OF UNFRACTIONATED Piper PLANT EXTRACTS BY HPLC-UV-DAD COUPLED WITH CHEMOMETRIC METHODS

Cited by 6 publications

References 23 publications

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

CHEMICAL STUDY AND ANTIMICROBIAL ACTIVITIES OF Piper arboreum (PIPERACEAE)

Quality assessment of crude and processed ginger by high‐performance liquid chromatography with diode array detection and mass spectrometry combined with chemometrics

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