Fingerprinting and Identification of Bacteria Present in UASB Granules Used to Treat Winery, Brewery, Distillery or Peach-lye Canning Wastewater

Abstract

The effective operation of the anaerobic digestion process in an upflow anaerobic sludge blanket (UASB) bioreactor is dependent on the microbial composition of the UASB granules. The granules contain a consortium of bacteria, with a specific metabolic function for each group, contributing to the overall efficiency and stability of the bioreactor. The aim of this study was to fingerprint and identify the bacteria present in four different types of South African UASB granules that are used to treat winery, brewery, distillery and peach-lye canning wastewaters. This was done by combining conventional microbiological platings with PCR-based denaturing gradient gel electrophoresis (DGGE) and DNA sequence analysis. Each granule type showed distinct PCR-based DGGE fingerprints with unique bands, while other bands were found to be present in all the granules, regardless of the wastewater being treated. Sixty-eight different bacteria (40 pure isolates and 28 clones) were partially sequenced and identified from the winery, brewery, distillery and peach-lye canning granules. Thirty-five percent of the identified bacteria represented the unculturable bacteria and 65% represented the culturable bacteria, which included members of the following genera:

Bioconversion of volatile fatty acids from macroalgae fermentation into microbial lipids by oleaginous yeast

“…Research using anaerobic fermentation of algal biomass has been conducted for more than 50 years [20]. Many studies have used macroalgae to produce methane, ethanol, and hydrogen [21].…”

“…Some Pseudomonas spp. are facultatively anaerobic, and some are known as aromatic hydrocarbon degrading [24], denitrifying [25] granular supporting bacteria [20]. Although Pseudomonas spp.…”

“…is mostly known as aerobic except for P. aeruginosa which can make anaerobic breath using other electron acceptors than oxygen in biofilms [26], there were still some research found that Pseudomonas could survive even under the anaerobic environment. Keyser et al, isolated P. veronii from upflow anaerobic sludge blanket (UASB) bioreactor granules [27]. In another research yconducted to analyze the consortium form the liquid phase of an anaerobic fixed-bed digester which was used to treat a cheese whey substrate, some strains of Pseudomonas were still isolated from the anaerobic digester such as P. fluorescens even using a bacteria-free substrate [28].…”

Methanogenic Microorganisms in Industrial Wastewater Anaerobic Treatment

“…A wide range of anaerobic bacteria are reported inhabiting anaerobic fermenters. These belongs mainly to the Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Spirochaetes, Chloroflexi, Planctomycetes a Synergistes [56][57][58][59][60][61]. Archaea represents approximately 5-6% of the total microbial population in anaerobic fermenters.…”

Análise dos parâmetros que interferem no metabolismo da microbiota anaeróbia e anóxica na remoção de bifenilas policloradas

“…Pseudomonas é caracterizada como microrganismos com metabolismo restrito que utilizam o oxigênio como receptor final de elétrons, entretanto, com alguns representantes capazes de utilizar nitrato como receptor alternativo, permitindo seu crescimento em condições anóxicas (DAUM et al, 1998;BOONE et al, 2005). Além disso, estes microrganismos foram identificados em grânulos de reatores UASB (ATUANYA; AIGBIRIOR, 2002;KEYSER;BRITZ;WITTHUHN, 2007). Assim, a elevada abundância relativa destes microrganismos em MT2 pode ser relacionada com a natural presença destes microrganismos no inóculo.…”