Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits

Abstract

Carvalho M., Matos M., Carnide V., 2014. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort. Sci. (Prague), 41: 175-184.In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivars using morphological and flavour traits are largely unsuccessful, due to environmental influences. The genetic similarity of ten highbush blueberry cultivars was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers from fruits and leaves. The DNA concentrations obtained in fruits and leaves were very similar and the band profiles observed in the two tissues were analogous with both molecular markers. RAPD analysis generated 144 bands, of which 112 were polymorphic (77.8%) in fruits and 141 bands of which 118 were polymorphic (83.7%) in leaves. In fruits, ISSR analysis produced 151 bands of which 127 were polymorphic (84.1%) and in leaves it produced 148 bands with 127 being polymorphic (85.8%). Dendrogram and principal coordinates analysis (PCO) analysis using the both markers results were concordant and a clear division of the types of highbush blueberry cultivars (northern and southern) into two distinct groups was verified.

Blueberry Cultivar Identification Using Random Amplified Polymorphic DNA and Sequence-characterized Amplified Region Markers

“…Most blueberry cultivars with common parents clustered together and reflected the genetic contributions of their parentage in the dendrogram. Carvalho et al (2014) clearly separated 10 highbush blueberry cultivars into northern and southern highbush blueberry types using RAPD and ISSR markers. However, northern and southern highbush blueberry cultivars were not clearly separated in this study, and some southern highbush blueberry cultivars clustered with northern highbush blueberry cultivars.…”

“…Burgher et al (2002) reported that 11 primers produced 73 polymorphic RAPD bands among 26 lowbush blueberry cultivars. Carvalho et al (2014) obtained 118 polymorphic bands using 10 selected primers among 10 highbush blueberry cultivars. Thus, the number of polymorphic bands in our study was smaller than those found in similar studies; the different number of polymorphic bands selected might account for using different primers and the genetic diversity in our blueberry cultivars.…”

Authentication of berries and berry‐based food products

“…To circumvent problems associated with RAPD, species‐specific RAPD fragments can be converted into the more reliable SCAR markers. RAPD and SCAR markers have been used for the fingerprinting of Vaccinium berries, such as blueberry and cranberry cultivars (Carvalho et al., 2014; Cho et al., 2017; Debnath, 2005; Levi & Rowland, 1997; Polashock & Vorsa, 1997, 2002). However, along with more economical sequencing services and the accumulation of specific sequencing data from different species, methods utilizing random primers are likely to get less popular in the future.…”

Foods with Functional Properties and their Potential Uses in Human Health

“…of fruits and leaves [123] (see Table 2). This fruit has a wide range of phenolic compounds, especially flavonols, such as quercetin, as well as anthocyanins [127].…”