Fingerprinting the genetic variation and intergeneric hybrid dynamics in the family Asteraceae (genera Helianthus, Echinaceae, Tagetes and Verbesina) using iPBS markers

Bonchev, Georgi; Vassilevska-Ivanova, R.

doi:10.2478/s11756-019-00363-3

Cited by 2 publications

(2 citation statements)

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“…In contrast to other retrotransposon-based molecular markers that require specific primers for the flanking regions of the particular retrotransposon, iPBS uses universal primers which enable the amplification of various types of LTRs. So far, there are several studies in which dominant iPBS markers were successfully applied for the estimation of genetic diversity and differentiation of plants (Bonchev and Vassilevska-Ivanova 2020 ; Khapilina et al 2021 ; Naeem et al 2021 ) and fungi (Ates et al 2019 ; Turzhanova et al 2020 ; Erper et al 2021 ). According to our knowledge, iPBS markers have only been used once for the genetic study of genus Avena , reporting genetic diversity and differentiation of Latvian A. fatua populations (Nečajeva et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

et al. 2023

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Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and expected heterozygosity (He) revealed low genetic diversity, with the highest values observed for A. nuda (I = 0.099; He = 0.068). UPGMA clustering of studied Avena accessions and PCoA results showed that the polyploidy level is the main grouping criterion. High-resolution gas chromatography revealed that the studied Avena accessions share the same composition of soluble carbohydrates, but significant differences in the content of total (5.30–22.38 mg g−1 of dry weight) and particular sugars among studied samples were observed. Sucrose appeared as the most abundant sugar (mean 61.52% of total soluble carbohydrates), followed by raffinose family oligosaccharides (31.23%), myo-inositol and its galactosides (6.16%), and monosaccharides (1.09%). The pattern of interspecific variation in soluble carbohydrates, showed by PCA, was convergent to that revealed by iPBS markers. Thus, both methods appeared as a source of valuable data useful in the characterization of Avena resources or in the discussion on the evolution of this genus.

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Section: Introductionmentioning

confidence: 99%

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

et al. 2023

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“…Thus, the iPBS retrotransposon technique has been selected as a marker to examine population genetic diversity and structure in many plant genera such as Cicer (Andeden et al 2013), Vitis (Guo et al 2014), Pisum (Baloch et al 2015), Phaseolus (Nemli et al 2015), Psidium (Mehmood et al 2017), Castanea, Fagus and Quercus (Coutinho et al 2018), Chenopodium (Hossein-Pour et al 2019), Laurus (Karık et al 2019), Hordeum (Bonchev et al 2019), Origanum (Karagoz et al 2020). Moreover, in Asteraceae iPBS method has been proved to be a reliable marker for the evaluation of genetic diversity at infraspecific level, as well as the intergeneric hybrid in several genera (Gailite and Rungis 2012;Ali et al 2019;Bonchev and Vassilevska-Ivanova 2020). To simplify the application of molecular tools and offer a better understanding of the genetic diversity of Cissampelopsis, we utilized iPBS molecular markers in these species for the first time.…”

Section: Introductionmentioning

confidence: 99%

Inter-primer binding site (iPBS) markers reveal the population genetic diversity and structure of tropical climbing Cissampelopsis (Asteraceae) in Thailand

Vanijajiva

Pornpongrungrueng

2020

Biodiversitas

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Abstract. Vanijajiva O, Pornpongrungrueng P. 2020. Inter-primer binding site (iPBS) markers reveal the population genetic diversity and structure of tropical climbing Cissampelopsis (Asteraceae) in Thailand. Biodiversitas 21: 3919-3928. Cissampelopsis is a small climbing tropical Asian genus of Asteraceae-Senecioneae. In Thailand, the genus is represented by two species, C. corifolia and C. Volubilis, distributed through the mountain evergreen forest. Study on the genetic diversity and structure of populations of both Cissampelopsis species provide better understanding of the biology and pattern of species diversification in the genus. To identify the genetic diversity, we used the inter-primer binding site (iPBS) retrotransposon system, in 96 accessions of Cissampelopsis species collected from different regions in Thailand. A total of 120 iPBS bands were scored as presence⁄ absence characters. Results from UPGMA and PCoA analyses indicated that C. corifolia and C. volubilis are different species. Genetic diversity and genetic differentiation among and within populations of C. volubilis is higher than C. corifolia. Molecular Variance (AMOVA) analysis of both species indicated that the genetic variance value within populations is higher than among populations of each species. Bayesian model-based STRUCTURE analysis detected two gene pools for both Cissampelopsis and showed admixture within individuals. Differences among the two Cissampelopsis species, in total diversities and levels of population differentiation, indicated that the genetic structure of Cissampelopsis populations are congruent with long-lived perennial habit with regional distribution, even for congeneric species, may vary considerably. This study suggests the effectiveness of the iPBS marker system to estimate the population genetic diversity and structure of Cissampelopsis genotypes.

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Fingerprinting the genetic variation and intergeneric hybrid dynamics in the family Asteraceae (genera Helianthus, Echinaceae, Tagetes and Verbesina) using iPBS markers

Cited by 2 publications

References 54 publications

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

Inter-primer binding site (iPBS) markers reveal the population genetic diversity and structure of tropical climbing Cissampelopsis (Asteraceae) in Thailand

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