The holding force acting on a levitated object in a near-field acoustic levitation apparatus was examined in an effort to increase acoustic levitation between opposing vibration sources. Using finite element analysis, we calculated holding force levels generated by vertically opposed vibration sources and found that when the phase difference of the opposing vibration sources was 180°, the holding force level was larger than could be produced by a single vibration source. On the other hand, when the phase difference is 0°, the holding force produced by acoustic radiation between the opposing vibration surfaces decreased. Additionally, we found that the holding force increased against the decrease of distance between the vibration surfaces. This increase in holding force between opposing vibration sources was experimentally confirmed by the position transient responses of an object detected via image processing. Hence, increases in the holding force levels produced by acoustic levitation between opposing vibration sources have been achieved.