We present the integration of a new calibration system into the Faint Intergalacticmedium Redshifted Emission Balloon-2 (FIREBall-2), which added in-flight calibration capability for the recent September 2023 flight. This system is composed of a calibration source box containing zinc and deuterium lamp sources, focusing optics, electronics, sensors, and a fiber-fed calibration cap with an optical shutter mounted on the spectrograph tank. We discuss how the calibration cap is optimized to be evenly illuminated through non-sequential modeling for the near-UV (191 to 221 nm) for spectrograph slit mask position calibration, electron multiplying charged-coupled device (EMCCD) gain amplification verification, and wavelength calibration. Then, we present the pre-flight performance testing results of the calibration system and their implications for in-flight measurements. FIREBall-2 flew in 2023, but did not collect calibration data due to early termination of the flight.