Fireblight (Erwinia amylovora) affects Mal d 1-related allergenicity in apple

Mayer, M; Oberhuber, Christina; Loncaric, Igor; Heissenberger, Birgit; Keck, M.; Scheiner, Otto; Hoffmann‐Sommergruber, Karin

doi:10.1007/s10658-011-9784-4

Cited by 11 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This low-level expression could be part of systemic acquired resistance of plants to prevent a further attack by biotrophic pathogens [44]. These differences in PR-protein and allergen encoding gene expression between leaves and fruits as well as between young and major plants have already been shown for different apple cultivars infected with fireblight [19]. The authors showed an up-regulation of major apple allergen Mal d 1.01 transcripts in leaves from seedlings after fireblight infection.…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Pepino mosaic virus Infection of Tomato Affects Allergen Expression, but Not the Allergenic Potential of Fruits

Welter

Dölle

Lehmann³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

The plant pathogen Pepino mosaic virus (PepMV) is a major disease of greenhouse tomato crops worldwide. Plant pathogens can induce expression of defence- or pathogenesis-related proteins, including identified allergens. Therefore we hypothesised that PepMV infection results in the expression of allergens leading to a higher allergenic potential of tomato fruits. Transcript level analyses showed differential expression of 17 known and putative tomato fruit allergen encoding genes at early and late time points after PepMV inoculation, but no general induction was detected. Immunoblot analyses were conducted and IgEs from a serum pool of tomato allergic subjects reacted with 20 proteins, of which ten have not yet been described. In parallel, skin prick tests with a group of tomato allergic subjects did not show a general difference between PepMV infected and non-infected tomato fruits and basophil activation tests confirmed these results. In summary, PepMV infection of tomato plants can lead to long-lasting up-regulation of particular allergens in fruits, but the hypothesis that this results in a higher allergenic potential of the fruits proved invalid.

show abstract

Section: Discussionmentioning

confidence: 95%

“…Interestingly, PR-proteins show high homologies to allergens and allergenic activity could be confirmed in many PR-protein families [17], [18]. For example, in apple an infection of young leaves from seedlings by fireblight, a bacterial disease, provoked an increase in the PR-protein and allergen Mal d 1.01 encoding gene [19].…”

Section: Introductionmentioning

confidence: 93%

Pepino mosaic virus Infection of Tomato Affects Allergen Expression, but Not the Allergenic Potential of Fruits

Welter

Dölle

Lehmann³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Interestingly, the two up-regulated proteins (spots 11 and 13) were annotated as the same protein, s-adenosylmethionine synthetase (SAMS), which serves as a precursor in polyamine biosynthesis [44]. In addition, three proteins were identified as the same heat-shock protein (spots 1–2 and 37), which is usually involved in the stress response.…”

Section: Resultsmentioning

confidence: 99%

“…The largest group contained proteins related to defence response, including 2 up-regulated proteins ( Fig 3 , spots 11 and 13) and 13 down-regulated proteins ( Fig 3 , spots 1–2, 10, 15, 19, 28, 31–32, and 36–40). Interestingly, the two up-regulated proteins (spots 11 and 13) were annotated as the same protein, s-adenosylmethionine synthetase (SAMS), which serves as a precursor in polyamine biosynthesis [ 44 ]. In addition, three proteins were identified as the same heat-shock protein (spots 1–2 and 37), which is usually involved in the stress response.…”

Section: Resultsmentioning

confidence: 99%

Proteome Analysis of Pathogen-Responsive Proteins from Apple Leaves Induced by the Alternaria Blotch Alternaria alternata

2015

View full text Add to dashboard Cite

Understanding the defence mechanisms used by apple leaves against Alternaria alternate pathogen infection is important for breeding purposes. To investigate the ultrastructural differences between leaf tissues of susceptible and resistant seedlings, in vitro inoculation assays and transmission electron microscopy (TEM) analysis were conducted with two different inoculation assays. The results indicated that the resistant leaves may have certain antifungal activity against A. alternate that is lacking in susceptible leaves. To elucidate the two different host responses to A. alternate infection in apples, the proteomes of susceptible and resistant apple leaves that had or had not been infected with pathogen were characterised using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS). MS identified 43 differentially expressed proteins in two different inoculation assays. The known proteins were categorised into 5 classes, among these proteins, some pathogenesis-related (PR) proteins, such as beta-1,3-glucanase, ascorbate peroxidase (APX), glutathione peroxidase (GPX) and mal d1, were identified in susceptible and resistant hosts and were associated with disease resistance of the apple host. In addition, the different levels of mal d1 in susceptible and resistant hosts may contribute to the outstanding anti-disease properties of resistant leaves against A. alternate. Taken together, the resistance mechanisms of the apple host against A. alternate may be a result of the PR proteins and other defence-related proteins. Given the complexity of the biology involved in the interaction between apple leaves and the A. alternate pathogen, further investigation will yield more valuable insights into the molecular mechanisms of suppression of the A. alternate pathogen. Overall, we outline several novel insights into the response of apple leaves to pathogen attacks. These findings increase our knowledge of pathogen resistance mechanisms, and the data will also promote further investigation into the regulation of the expression of these target proteins.

show abstract

“…Similarly, ethylene associated to tissue wounding provoked an increase of the allergenic potency of turnip by inducing the expression of a prohevein [18]. Another example is given by the work of Mayer et al [19], who demonstrated the increase of Mal d 1 gene transcription and protein accumulation after infection by Erwinia amylovora . In contrast, a recent publication [20] showed that the infection of tomato by Pepino mosaic virus, despite inducing the overexpression of specific allergenic proteins, did not lead to a parallel increase of the allergenic potency of the fruit, analyzed by basophil activation test.…”

Section: Discussionmentioning

confidence: 99%

Grapevine Downy Mildew <b><i>Plasmopara viticola</i></b> Infection Elicits the Expression of Allergenic Pathogenesis-Related Proteins

Rossin

Villalta

Martelli

et al. 2015

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Downy mildews are a group of microorganisms belonging to the Chromista kingdom that can infect specific plants. When growing on plant tissues these microbes can elicit the expression of pathogenesis-related proteins (PRs), a group of stress-induced proteins frequently described as allergens in many plant species. Our aim was to verify by a proteomic approach whether the allergic reactions experienced by a farmer working in a vineyard infected by Plasmopara viticola (Pv), the etiological agent of downy mildew, are elicited by PRs expressed by the grapevine upon infection or by allergens present in Pv. Methods: A skin prick test and prick-to-prick test with infected field grapevine leaves and control leaves were carried out. Field leaves and ad hoc Pv-inoculated leaves were compared by SDS-PAGE and IgE-immunoblotting with extracts from control leaves and Pv sporangia. IgE-binding proteins were further separated by two-dimensional electrophoresis and the positive spots analyzed by nanoHPLC-Chip and tandem mass spectrometry (MS/MS) for identification. Results: Only infected leaves showed IgE-binding protein bands at 42 and 36 kDa. This agreed with the positive skin prick test experienced by the patient only with the infected leaves extract. Two-dimensional electrophoresis followed by MS/MS analysis led to the identification of PR-2 (β-1,3-glucanase) and harpin-binding protein 1 as putative allergens, the latter having never been reported before. Conclusion: The results indicate that Pv infection might represent a new source of plant allergens.

show abstract

Fireblight (Erwinia amylovora) affects Mal d 1-related allergenicity in apple

Abstract: Fireblight infection of apple and pear trees

Cited by 11 publications

References 31 publications

Pepino mosaic virus Infection of Tomato Affects Allergen Expression, but Not the Allergenic Potential of Fruits

Pepino mosaic virus Infection of Tomato Affects Allergen Expression, but Not the Allergenic Potential of Fruits

Proteome Analysis of Pathogen-Responsive Proteins from Apple Leaves Induced by the Alternaria Blotch Alternaria alternata

Grapevine Downy Mildew <b><i>Plasmopara viticola</i></b> Infection Elicits the Expression of Allergenic Pathogenesis-Related Proteins

Contact Info

Product

Resources

About