First and Second Derivative Spectrophotometric Determination of Flavonoids Chrysin and Quercetin

Nikolovska‐Coleska, Zaneta; Klisarova, L.; Šuturkova, Lj.; Dorevski, K.

doi:10.1080/00032719608000395

Cited by 43 publications

(7 citation statements)

References 20 publications

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“…146 Specificity can be enhanced in direct spectrophotometric methods by derivative spectrometry. For instance, chrysin and quercetin were determined 151 spectrophotometrically using first-and second-derivative spectra in a method that requires no preliminary separation of the flavanoids.…”

Section: Total Phenolsmentioning

confidence: 99%

Analytical Chemistry of Fruit BioflavonoidsA Review

Robards¹,

Antolovich²

1997

Analyst

438

267

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Section: Total Phenolsmentioning

confidence: 99%

Analytical Chemistry of Fruit BioflavonoidsA Review

Robards¹,

Antolovich²

1997

Analyst

438

267

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“…3,4 In this context, various analytical methods have been reported in literature for determination of some flavonoids. These include UV-Vis absorption, spectrophotometry, 5 chromatographic methods like thinlayer chromatography (TL), gas chromatography (GC), liquid chromatography (LC) and capillary electrophoresis (CE) with UV or electrochemical detection, [6][7][8][9][10][11] based on the oxidation of the phenolic hydroxyl groups in flavonoids. Direct electroanalytical determination of flavonoids by differential pulse voltammetry or stripping techniques has only rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Square-wave cathodic adsorptive stripping voltammetric determination of 3-hydroxyflavone, Morin and Hesperidin in bulk form and biological fluids in absence and presence of Cu(II)

Temerk¹,

Ibrahim²,

Kotb³

2011

J. Braz. Chem. Soc.

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Devido à atividade farmacológica dos flavonóides, na presente investigação, foi desenvolvido um método eficiente e econômico para a determinação de traços dos mesmos. Neste contexto, o comportamento interfacial de três flavonóides, 3-hidroxiflavona (3HF), morin e hesperidina (Hesp) foi estudado em eletrodo de gota pendente de mercúrio (HMDE) por voltametria ac (fase sensível) e voltametria cíclica (CV). Os flavonóides investigados mostraram-se fortemente absorvidos, o que é um pré-requisito para a aplicação de voltametria adsortiva de redissolução catódica, na determinação de alguns flavonóides. Baseado no caráter de adsorção dos flavonóides investigados, na superfície de HMDE, foi descrito um procedimento de voltametria adsortiva de redissolução catódica com onda quadrada (SWCASV), validado, simples, rápido e sensível, para a quantificação dos flavonóides sob investigação na forma granel e em fluidos biológicos. Além disso, a acumulação adsortiva controlada do complexo de Cu(II) de flavonóides foi também obtida e usada para a determinação indireta de 3HF, morin e Hesp via redução do complexo formado. As condições operacionais e de solução para a determinação quantitativa ultra-traço dos compostos flavonóides investigados, foram otimizadas na ausência e presença de Cu(II). Os limites de detecção de 4,4 × 10 -9 , 7,19 × 10 -9 e 7,54 × 10 -9 mol L -1 para 3HF, morin e Hesp, respectivamente, na forma granel, foram atingidos usando SWCASV.As flavonoids show pharmacological activity, in the present investigation, an economical and efficient method for their trace determination was developed. In this context, the interfacial behavior of three flavonoids, 3-hydroxyflavone (3HF), morin and hesperidin (Hesp) was studied on the hanging mercury dropping electrode (HMDE) by phase sensitive ac voltammetry and cyclic voltammetry (CV). The investigated flavonoids were strongly adsorbed which is the prerequisite step for applying the cathodic adsorption stripping voltammetric determination of some flavonoids. Based on the adsorption character of investigated flavonoids onto the surface of the HMDE, validated, simple, fast and sensitive square-wave cathodic adsorptive stripping voltammetric (SWCASV) procedure for the quantification of the flavonoids under investigation in bulk form and in biological fluids was described. Moreover, controlled adsorptive accumulation of the Cu(II) complex of flavonoids was also achieved to assay indirect determination of 3HF, morin and Hesp via the reduction of the formed complex. Operational and solution conditions for the quantitative ultra-trace determination of the investigated flavonoids were optimized in the absence and presence of Cu(II). Limits of detection of 4.4 × 10 -9 , 7.19 × 10 -9 and 7.54 × 10 -9 mol L -1 3HF, morin and Hesp in bulk form were achieved using SWCASV, respectively.

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“…Chlorpheniramine maleate and codeine phosphate were determined simultaneously with linear concentra-tion ranges of 0.001-0.08 and 0.001-0.4 mg/mL, respectively, while ephedrine hydrochloride was determined after oxidation with sodium periodate with a linear concentration range of 0.005-1.80 mg/mL. A new spectrophotometric method for the analysis of binary mixtures of the flavonoids chrisin and quercetin using first-and second-order derivatives of the spectra ratio has been described (118). The third-derivative method using the zerocrossing technique has been employed to effect the simultaneous determination of (dimethylamino)ethyl (o-chloro-p-(dimethylamino)sulfonylphenoxy)acetate hydrochloride and its major hydrolytic decomposition product (o-chloro-p-(dimethylamino)sulfonylphenoxy)acetic acid (119).…”

Section: Chemistrymentioning

confidence: 99%