First Characterization of a Cluster of VanA-Type Glycopeptide-Resistant<i>Enterococcus faecium,</i>Colombia

Panesso, Diana; Ospina, Sigifredo; Robledo, Jaime; Vela, María Claudia; Peña, Julieta; Hernández, Orville; Reyes, Jinnethe; Arias, César A.

doi:10.3201/eid0809.010435

Cited by 23 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, they were highly resistant to aminoglicosides, limiting thus the success of such asso-ciated antibiotic therapy. The concomitant resistance observed to chloramphenicol, penicillin, erythromycin, teicoplanin, ciprofloxacin, and tetracycline confirms that resistance of enterococci to multiple antibiotics is common, as also observed by Panesso et al (2002). Linezolide was highly effective against all the isolates, indicating it as an appropriate therapeutic option.…”

Section: Discussionsupporting

confidence: 58%

Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

Vilela

Souza

Palazzo

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 58%

Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

Vilela

Souza

Palazzo

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…It is probable that Klebsiella strains with MDR phenotype carry the co-presence of chloramphenicol resistance determinants (e.g., cat genes) with other resistance determinants in transposons on plasmids [90]. [12] 2002 Serbia 44 E. faecium KB 2% VR 73 [13] 2002 Brazil 23 E. faecium E-test Broth-MD 100% VR 78 [14] 2002 UK 54 E. faecium Broth-MD 57.4% VR 55 † [15] 2002 Colombia 23 E. faecium VITEK system 100% VR 100 [16] 2003 NA 616 E. faecium Broth-MD 100% VR 99.7 [17] 2003 NA 1997 (Y): 214 E. faecium VITEK system Broth-MD 43% VR 90 [18] 1998 ( [54] 2008 Korea 16 MicroScan 100 [55] 2009 Nepal 63 KB 92 [56] 2009 Pakistan 42 KB 67 [57] 2009 Greece 27 § KB 100 [58] 2009 China 129 Agar dilution 99 [ …”

Section: Klebsiella Pneumoniaementioning

confidence: 99%

Could chloramphenicol be used against ESKAPE pathogens? A review ofin vitrodata in the literature from the 21st century

Čivljak

Giannella

Bella

et al. 2014

Expert Review of Anti-infective Therapy

View full text Add to dashboard Cite

The widespread use of antibiotics has been associated with the emergence of antimicrobial resistance among bacteria. 'ESKAPE' (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acintobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) pathogens play a major role in the rapidly changing scenario of antimicrobial resistance in the 21st century. Chloramphenicol is a broad spectrum antibiotic that was abandoned in developed countries due to its association with fatal aplastic anemia. However, it is still widely used in the developing world. In light of the emerging problem of multi-drug resistant pathogens, its role should be reassessed. Our paper reviews in vitro data on the activity of chloramphenicol against ESKAPE pathogens. Susceptibility patterns for Gram-positives were good, although less favorable for Gram-negatives. However, in combination with colistin, chloramphenicol was found to have synergistic activity. The risk-benefit related to chloramphenicol toxicity has not been analyzed. Therefore, extra precautions should be taken when prescribing this agent.

show abstract

“…Sin embargo, las bacterias de este género son aislamientos de nosocomios, principalmente de pacientes con endocarditis, bacteriemias e infecciones urinarias (1)(2)(3). Lo anterior se ve agravado por el incremento, incluso en Colombia, del número de aislamientos resistentes a los antibióticos comúnmente empleados en el tratamiento de estas infecciones (4,5).…”

Section: De Cultivos Huvec Por Enterococcus Spunclassified

“…Encontramos que al exponer las células a las bacterias por dos horas y luego tratarlas con antibióticos para eliminar E. faecalis extracelulares o adheridos a la membrana, es posible encontrar bacterias en el interior de las HUVEC cultivadas. (4). La cepa no invasiva Escherichia coli DH5a sirvió como control negativo en los experimentos de infección.…”

Section: De Cultivos Huvec Por Enterococcus Spunclassified

Cultivos primarios de células endoteliales aisladas de cordón umbilical humano: un modelo biológico para el estudio de los mecanismos de infección por enterococos.

Chiriboga

Fontanilla

2004

biomedica

View full text Add to dashboard Cite

Aunque las especies de enterococos son flora normal del tracto gastrointestinal humano, se encuentran entre los tres agentes patógenos microbianos que más se asocian con infecciones intrahospitalarias. Tradicionalmente, los enterococos se han considerado bacterias extracelulares. Sin embargo, es creciente la información que reporta su ingreso a través de líneas celulares epiteliales o macrófagos. A pesar de que estos microorganismos constituyen el tercer grupo de aislamientos obtenido de pacientes con bacteriemia o endocarditis, su interacción con la célula endotelial no se ha descrito completamente. En el presente trabajo evaluamos si el aislamiento intrahospitalario de Enterococcus faecalis (Ef2880) podía penetrar en células endoteliales de la vena de cordón umbilical humano (HUVEC) cultivadas in vitro. Nuestros resultados indican que los cultivos primarios HUVEC después de ser inoculados con Ef2890, incubados y tratados con antibióticos bactericidas para las bacterias extracelulares y adheridas a la cara externa de la membrana celular, exhiben bacterias intracelulares que pueden ser recuperadas vivas cuatro horas después de la inoculación. El modelo biológico desarrollado se puede constituir en una herramienta útil para el estudio de las interacciones que establecen los enterococos con el endotelio.Palabras clave: endotelio, cultivo celular, Enterococcus sp., infección enterocócica de cultivos de HUVEC Primary cultures of human umbilical chord vein endothelial cells: a biological model for studying enterococcal infection mechanismsAlthough enterococcus bacteria are normal human intestinal flora, they rank as the third most common pathogen involved in hospital acquired infections. Generally, these bacteria are considered extracellular pathogens; however, an increasing number of reports indicate invasiveness to epithelial cell lines and macrophages. Despite their importance as nosocomial infection agents in patients suffering bacteremias and endocarditis, their interaction with endothelial cells has not been fully described. Herein, the nosocomial Enterococcus faecalis isolate Ef2890 from a hospitalized patient was exposed to cultured human venous endothelial cells from the umbilical chord. When the primary cell cultures were inoculated with Ef2890 and treated with bactericidal antibiotics to kill extracellular and adhered bacteria, intracellular bacteria were recovered and plated 4 h post-infection. These observations indicate that cell cultures provide a valuable biological model to study interactions between endothelium and enterococci.

show abstract

First Characterization of a Cluster of VanA-Type Glycopeptide-ResistantEnterococcus faecium,Colombia

Cited by 23 publications

References 42 publications

Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

Could chloramphenicol be used against ESKAPE pathogens? A review ofin vitrodata in the literature from the 21st century

Cultivos primarios de células endoteliales aisladas de cordón umbilical humano: un modelo biológico para el estudio de los mecanismos de infección por enterococos.

Contact Info

Product

Resources

About