First Complete Genome Sequence of
            <i>Cucumber green mottle mosaic virus</i>
            Isolated from Australia

Kehoe, Monica A.; Jones, R. A. C.; Coutts, B. A.

doi:10.1128/genomea.00036-17

Cited by 9 publications

(3 citation statements)

References 11 publications

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“…Viral RNA extraction (using Viral RNA Extraction Kit; Bioneer) from ToBRFV-infected tomato (S. lycopersicum) seeds (Luria and Dombrovsky, unpublished data) and CGMMV-infected cucumber (C. sativus Derben) and melon (C. melo Raanan) seeds were successfully executed [60]. Nextgeneration sequencing (NGS) platform has been successfully applied for detection of the tobamoviruses ToBRFV [6] and CGMMV [77]. The NGS detection method is highly sensitive when compared to the most commonly used serological assays (enzyme-linked immunosorbent assay, Western blot) and the genome sequence analysis performed after PCR amplification.…”

Section: Management Strategies 61 Sensitive Tobamovirus Detection Methodsmentioning

confidence: 99%

Aspects in Tobamovirus Management in Intensive Agriculture

Smith¹,

Dombrovsky²

2020

Plant Diseases - Current Threats and Management Trends

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In the recent years, disease spread of old and newly evolved tobamoviruses has occurred worldwide, affecting production of various vegetable and ornamental crops. The tobamoviruses are highly stable plant viruses that could cause severe disease symptoms. The well-known tobamovirus Cucumber green mottle mosaic virus (CGMMV) has recently caused severe damages in the cucumber, melon, and watermelon cucurbitaceous crops, worldwide. Similarly, a recent widespread of the newly identified tobamoviruses, Tomato mottle mosaic virus (ToMMV) and Tomato brown rugose fruit virus (ToBRFV), has reduced the solanaceous crop production. The primary route of tobamoviral infection is through mechanical means. These viruses adhere to agricultural facilities, contaminate the soil, infect seeds, and spread via beneficial pollinators and irrigation water. Mechanical plant injury suffices to initiate viral infection. Practicing hygiene by plant growers and in nurseries is currently the main strategy for mitigation of tobamoviral infection. Promoting the production of solanaceous vegetable crops genetically resistant to ToMMV and ToBRFV infection is a promising approach. However, CGMMV-resistant sources of cucurbitaceous vegetable crops are scarce. Conferring resistance to rootstocks and cross-protection strategies are newly implemented approaches that could alleviate tobamovirus disease spread in both solanaceous and cucurbitaceous crops.

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Section: Management Strategies 61 Sensitive Tobamovirus Detection Methodsmentioning

confidence: 99%

Aspects in Tobamovirus Management in Intensive Agriculture

Smith¹,

Dombrovsky²

2020

Plant Diseases - Current Threats and Management Trends

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“…Since the initial detection in the NT in 2014, CGMMV has been found in Western Australia, isolated areas in Queensland [ 2 , 11 ], South Australia ( (accessed on 27 May 2021)) and New South Wales. Following the initial detections in the NT, commercial cucurbit properties found to be positive for the virus were put under quarantine for a period of two years.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Longevity and Infectivity of Cucumber green mottle mosaic virus in Soils of the Northern Territory, Australia

Lovelock

Mintoff²,

Kurz³

et al. 2022

Plants

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Cucumber green mottle mosaic virus (CGMMV) is a Tobamovirus of economic importance affecting cucurbit crops and Asian cucurbit vegetables. CGMMV was detected in the Northern Territory (NT) in September 2014, the first record for Australia, with 26 properties confirmed as of May 2016. Research was undertaken to determine virus longevity in soils in the NT and investigate the use of disinfectants to remove viable CGMMV from the soil. An in-field trial at 12 months post-quarantine at four properties, and bioassays from collected soils indicate that CGMMV remained viable in at least two of the properties 12 months after plant hosts were removed from the ground. The infectivity of CGMMV from soil was also investigated in two trials with 140 watermelon seeds and 70 watermelon plants sown into CGMMV infested soils with or without the application of the disinfectants VirkonTM (2%) and Bleach (1%). Watermelons grown in soil, not treated with the VirkonTM or Bleach, showed CGMMV infection rates of 4% and 2.5% respectively. When VirkonTM or Bleach was applied, no positive CGMMV detections were observed in the watermelons. This research highlights the importance of proper management of infested properties and the need for on-farm biosecurity to manage CGMMV.

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“…CGMMV often causes leaf mosaic and malformation, fruit mottling and plant stunting in numerous plant species, including those in the families Solanaceae, Cucurbitaceae and Cruciferae, leading to severe damages to the vegetable industry worldwide [16][17][18]. CGMMV is originally identified in cucumber plant in England, and it is now extensively distributed in Asia, Australia and America [19][20][21][22][23][24][25][26]. Similar to other tobamoviruses, CGMMV is mainly transmitted via contacts and seeds in the field [27].…”

Section: Introductionmentioning

confidence: 99%

Physical contact transmission of Cucumber green mottle mosaic virus by Myzus persicae

Wang

et al. 2021

PLoS ONE

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Cucumber green mottle mosaic virus (CGMMV), a critical plant virus, has caused significant economic losses in cucurbit crops worldwide. It has not been proved that CGMMV can be transmitted by an insect vector. In this study, the physical contact transmission of CGMMV by Myzus persicae in Nicotiana benthamiana plants was confirmed under laboratory conditions. The acquisition rate increased with time, and most aphids acquired CGMMV at 72 h of the acquisition access period (AAP). Besides, the acquired CGMMV was retained in the aphids for about 12 h, which was efficiently transmitted back to the healthy N. benthamiana plants. More importantly, further experiments suggested that the transmission was mediated by physical contact rather than the specific interaction between insect vector and plant virus. The results obtained in our study contribute to the development of new control strategies for CGMMV in the field.

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First Complete Genome Sequence of Cucumber green mottle mosaic virus Isolated from Australia

Cited by 9 publications

References 11 publications

Aspects in Tobamovirus Management in Intensive Agriculture

Aspects in Tobamovirus Management in Intensive Agriculture

Investigating the Longevity and Infectivity of Cucumber green mottle mosaic virus in Soils of the Northern Territory, Australia

Physical contact transmission of Cucumber green mottle mosaic virus by Myzus persicae

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