First detection of emerging HoBi-like Pestivirus (BVD-3) among some persistently infected dairy cattle herds in Egypt

Abstract

Bovine viral diarrhoea virus (BVDV) is a serious veterinary health concern worldwide. We conducted this study to determine the prevalence of persistent infections (PI) and identify the current strain among some dairy cattle herds in Egypt. A total of 240 serum samples were collected from six Egyptian provinces. Between 2019 and 2020, samples were tested by Enzyme linked immunosorbent assay (ELISA) for detection of PI animals, and then molecular characterization was performed. Six calves were found PI with a prevalence of 2.5% (6/240). Using molecular characterization, HoBi-like Pestivirus (BVD-3) was successfully identified in Egypt for the first time. Based on the BVD-3 reference strains on Genbank, the detected strains had an identity ranging from 98.8 to 99.6%. Partial nucleotide sequence of the 5′UTR gene for six tested samples was submitted to Genbank with accessions: OM324396, OM324397, OM324398, OM324399, OM3243100, and OM3243101.

Development and Application of a Rapid Test for Detection of Bovine Viral Diarrhea Virus-specific Antibodies

“…Due to the challenges associated with virus isolation, the majority of research concentrates on nucleic acid detection in the 5'-UTR regions for identifying and classifying the virus. This results in a lack of reliable evidence for BVDV genotyping and comprehensive genetic characteristics analysis (Chang et al 2021;Afify et al 2022).…”

Detection of emerging HoBi-like Pestivirus (BVD-3) during an epidemiological investigation of bovine viral diarrhea virus in Xinjiang: a first-of-its-kind report

“…Subsequently, we utilized BVDV-1/2/3 primers to further test the No.5 sample, which generated the expected band at 296 bp, tentatively confirming that the No.5 sample was infected with BVDV-3 ( Figure 3B ). Because of the difficulty of virus isolation, most of the research focuses on the nucleic acid detection in 5’-UTR regions for virus identification and classification, which leads to the lack of reliable evidence for BVDV genotyping and comprehensive genetic characteristics analysis ( Hou et al, 2019 ; Chang et al, 2021 ; Tian et al, 2021 ; Afify et al, 2022 ). To obtain conclusive results regarding the No.5 sample’s infection with BVDV-3, we designed full-length BVDV amplification primers with reference to the sequence of BVDV-3 strain PB22487 (GenBank: KY762287.2), and successfully amplified the target fragment in a segmented manner ( Figure 3C ).…”