First detection of etoxazole resistance in Australian two‐spotted mite <i>Tetranychus urticae</i> Koch (Acarina: Tetranychidae) via bioassay and DNA methods

Herron, Grant A; Woolley, Lauren K.; Langfield, Kate L

doi:10.1111/aen.12290

Cited by 17 publications

(21 citation statements)

References 13 publications

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“…The CHS1 mutation has been previoulsy reported in only one strain from Turkey (Ilias et al 2014), but without matching toxicity data. Although significant fitness cost has been reported in the presence of the I1017F mutation (Bajda et al 2018), the mutation has been reported in T. urticae strains from different continents (Demaeght et al 2014;Ilias et al 2014;Adesanya et al 2018;Herron et al 2018). With regard to resistance management, our data suggest that more modern CHS1 inhibitors, like etoxazole that is in use throughout Europe, are not a good option to control T. urticae population in Turkey.…”

Section: Discussionmentioning

confidence: 70%

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

et al. 2019

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Tetranychus urticae Koch is one of the most common and harmful pests in vegetable production areas. Similar to other countries, control of T. urticae is mainly based on acaricides in Turkey. However, T. urticae rapidly develops resistance and failures in chemical control have occurred frequently. The toxicity of various acaricides was investigated in 10 T. urticae populations collected from vegetable crops in Turkey. In addition, populations were screened for the presence of all currently known target-site resistance mutations. It was shown that resistance to bifenthrin was the most widespread, but also half of the populations were resistant to abamectin and hexythiazox. Resistance mutations in the voltage-gated sodium channel (VGSC) and chitin synthase 1 were found in various populations. Moreover, for the first time, F1538I and L1024V VGSC mutations were reported for Turkish populations. Mutations that confer resistance to abamectin, bifenazate and METI-I acaricides such as pyridaben were not detected. These results will contribute to the design of an effective resistance management program in Turkey.

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Section: Discussionmentioning

confidence: 70%

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

et al. 2019

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“…2004; Herron et al . 2018). A second reference strain of T. urticae known as Nashee p was field collected in 2015 from a commercial fruit crop at Banbartha, Victoria, and carries the I1017F mutation in chitin synthase (CHS1) that causes etoxazole resistance (Herron et al .…”

Section: Methodsmentioning

confidence: 99%

“…A second reference strain of T. urticae known as Nashee p was field collected in 2015 from a commercial fruit crop at Banbartha, Victoria, and carries the I1017F mutation in chitin synthase (CHS1) that causes etoxazole resistance (Herron et al . 2018). A reference strain of T. lambi known as Carb that is insecticide susceptible was collected from cotton in Boggabilla, NSW, in 2017.…”

Section: Methodsmentioning

confidence: 99%

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Development and use of a single real‐time PCR assay to identify the three spider mite species Tetranychus urticae, Tetranychus lambi and Tetranychus ludeni (Acari: Tetranychidae)

et al. 2020

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Tetranychid spider mites are a significant worldwide pest of agriculture with Tetranychus urticae being the most important. In Australian cotton, T. urticae is the most damaging, but Tetranychus ludeni and Tetranychus lambi are also present and can be difficult to distinguish morphologically. For this reason, a fast‐diagnostic assay was developed to identify T. urticae, T. lambi and T. ludeni in a single real‐time PCR reaction. The assay design was based on a universal primer pair to amplify the internal transcribed spacer of nuclear ribosomal DNA (ITS1) with three species‐specific TaqMan probes. The assay was validated by sequencing three known reference strains for T. urticae, T. lambi and T. ludeni and a blind test on 14 DNA samples that included species other than the three target species. The method correctly identified the target species and produced a negative result against other non‐target species. Furthermore, we examined 22 field‐collected spider mite populations from Australian cotton that had been laboratory maintained. Here, we found that 50% of the populations labelled as T. urticae were mixed with T. lambi and quarter of the populations labelled with T. lambi contained T. urticae. It demonstrates just how difficult it is to maintain strain integrity with multiple species in proximity. Finally, the assay was used by Biosecurity Australia to test 2125 mite quarantine intercept specimens. They found 1949 T. urticae, 1 T. lambi, 13 T. ludeni and 162 unknown specimens confirming assay accuracy and robustness. The diagnostic assay developed can provide quick spider mite species identification and can support multiple uses including species identification to support spray decisions or the precise identification of border quarantine intercepts where accuracy and turnaround time is critical.

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“…It acts as a mite growth inhibitor and inhibits chitin synthesis in embryonic and immature stages (Demaeght et al, 2014;Van Leeuwen et al, 2012). There are many reports of etoxazole resistance in spider mites (Adesanya, et al, 2018;Demaeght et al, 2014;Herron et al, 2018;Lee et al, 2004;Wu et al, 2019). In T. urticae, an amino acid substitution of isoleucine to phenylalanine at position 1017 (I1017F) in the chitin synthase 1 gene (CHS1) has been confirmed to confer strong etoxazole resistance (Demaeght et al, 2014;Douris et al, 2016;Herron et al, 2018;Riga et al, 2017;Van Leeuwen et al, 2012;Wu et al, 2019).…”

Section: Several Chemical Classes Of Acaricides With Different Modes mentioning

confidence: 99%

Metabolic mechanisms of resistance to spirodiclofen and spiromesifen in Iranian populations of Panonychus ulmi

et al. 2020

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The European red mite, Panonychus ulmi (Koch), is one of the major pests of apple trees worldwide. Cyclic keto-enol compounds such as spirodiclofen and spiromesifen are frequently used to control phytophagous spider mites in agricultural crops, including P. ulmi on apple trees. Spider mites, however, can rapidly develop resistance against acaricides and, in this study, multiple P. ulmi populations from apple orchards in Iran were monitored for spirodiclofen and spiromesifen resistance. The Urmia and Shahin Dej population showed the highest spirodiclofen resistance ratio (more than 150-fold) compared to the susceptible Ahar population. Toxicity bioassays also revealed the presence of moderate cross-resistance between spiromesifen and spirodiclofen, but not towards the chitin synthase inhibitor etoxazole. As a first step towards elucidating spirodiclofen resistance mechanisms, the role of detoxification enzymes (cytochrome P450 monooxygenases, carboxyl/choline esterases and glutathione S-transferases) was investigated by in vivo synergism and in vitro enzyme assays. PBO pretreatment synergized spirodiclofen toxicity in the populations of Urmia and Shahin Dej to a higher extent than in the susceptible Ahar population. Furthermore, enzyme activity measurements showed relatively higher activity of detoxifying enzymes in the resistant populations. In conclusion, increased detoxification is most likely underlying spirodiclofen resistance and results in limited crossresistance to spiromesifen.

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First detection of etoxazole resistance in Australian two‐spotted mite Tetranychus urticae Koch (Acarina: Tetranychidae) via bioassay and DNA methods

Cited by 17 publications

References 13 publications

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Development and use of a single real‐time PCR assay to identify the three spider mite species Tetranychus urticae, Tetranychus lambi and Tetranychus ludeni (Acari: Tetranychidae)

Metabolic mechanisms of resistance to spirodiclofen and spiromesifen in Iranian populations of Panonychus ulmi

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