First Detection of Resistance Genes Encoding Extended Spectrum Beta-Lactamase Producing Escherichia coli at Lome, Togo

Hr, Nagaraja; Deshmukh, Anand; Shivanna, Yathish; Ks, Thungappa; Rohit, Soumya

doi:10.4172/1989-8436.100062

Cited by 10 publications

(13 citation statements)

References 45 publications

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“…Imipenem was the most effective antimicrobial against E.coli, with a resistance rate of 9.09%. Resistance rates between the ranges 0% to 10% by E.coli to imipenem are globally comparable with several studies [9,[46][47][48]. Gentamicin proved to be the second most effective antimicrobial against both Klebsiella and E. coli isolates; including ESBL producers.…”

Section: Resultssupporting

confidence: 77%

Multi-Drug Resistant (MDR) Bacterial Isolates on Close Contact Surfaces and Health Care Workers in Intensive Care Units of a Tertiary Hospital in Bulawayo, Zimbabwe

Mbanga

Sibanda

Rubayah

et al. 2018

JAMMR

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Aims:A cross sectional study was conducted to determine the degree of microbial contamination of environmental surfaces and health-care workers (HCWs) in two multi-bed ICUs at a tertiaryreferral hospital in Bulawayo, Zimbabwe. Place and Duration of Study: Methodology: In total 64 surfaces were swabbed in two ICUs; inclusive of 6 hand swabs obtained from on-duty nurses. Fungal and bacterial isolates were identified using standard microbiological methods. Bacterial antibiogram profiles were determined by the Kirby-Bauer disc diffusion method. Mbanga et al.; JAMMR, 27(2): 1-15, 2018; Article no.JAMMR.42764 2 PCR was used to determine the presence of Extended Spectrum Beta Lactamase (ESBL) genes in isolated Enterobacteriaceae and the presence of the mecA gene in Staphylococcus aureus isolates. Results: Out of the 58 fomites and medical devices swabbed 50 (86.21%) were positive for bacterial contamination, with coagulase negative Staphylococci (CoNS) (20.31%) and Klebsiella species (20.31%) being the most prevalent environmental isolates. All 6 (100%) hand swabs obtained from the HCWs were positive for microbial contamination. A total of 51 (75%) of the 68 bacterial isolates were resistant to at least 3 antibiotics and 39 multi-drug resistance patterns were exhibited by the MDR isolates. Combinations of the ESBL genes blaCTX-M, blaTEM and blaSHV were detected in Escherichia coli, Klebsiella spp and Pseudomonas aeruginosa isolates. Methicillin-resistant Staphylococcus isolates (MRSA) were detected using phenotypic and molecular methods. Conclusion: ICU inanimate surfaces and medical equipment in the adult and paediatric ICUs of the referral hospital were heavily contaminated with MDR bacteria that could predispose critically ill patients to acquire nosocomial infections. Original Research Article

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Section: Resultssupporting

confidence: 77%

Multi-Drug Resistant (MDR) Bacterial Isolates on Close Contact Surfaces and Health Care Workers in Intensive Care Units of a Tertiary Hospital in Bulawayo, Zimbabwe

Mbanga

Sibanda

Rubayah

et al. 2018

JAMMR

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“…The prevalence of multiple drug resistance (MDR) among the studied isolates was 98.04%. This is considered to be very high and is in accordance with the reported findings of 85-100% in various parts of Nigeria and other African countries [28][29][30][31][32]. Hassan et al and Elsayed et al [33,14] also reported 100% and 95% MDR phenotypes respectively.…”

Section: Discussionsupporting

confidence: 89%

Prevalence and molecular characterization of extended spectrum beta-lactamase producing uropathogenic Escherichia coli in a tertiary health care facility in Anambra State, Nigeria

Nwafuluaku¹,

Agbakoba²,

Ushie³

et al. 2021

World J. Adv. Res. Rev.

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Background: The prevalence of ESBLs-producing Escherichia coli has recently increased worldwide due to the expression of ESBL genes which had led to high rate of multidrug resistance antibiotics. Aim: To determine the antimicrobial susceptibility patterns of E. coli isolates and evaluating the ESBL carriage of these isolates at phenotypic and genotypic levels. Methods: One hundred and two clinical Escherichia coli isolates were recovered from UTI suspects and analyzed for ESBL production at phenotypic and genotypic levels using Modified Double Disc Synergy Test and Polymerase Chain Reaction respectively. Results: Of the 102 isolates, 100(98.04%) were associated with MDR phenotypes. The isolates showed variable resistance to all the antibiotics used in the study. The resistance rates were 99.0%, 97.1%, 88.2%, 82.4%, 81.4%, 65.7%, 54.9%, 46.1%, 46.1%, 23.5% for ampicillin, trimethoprim-sulfamethoxazole, ciprofloxacin, ceftriaxone, ceftazidime, amoxicillin-clavulanate, gentamycin, cefoxitin, nitrofurantoin and imipenem, and respectively. The prevalence of phenotypic ESBL production was 74.5%. Based on the PCR results, the randomly selected 20 ESBL-positive isolates possessed one or more ESBLs genes. CTX-M type was the predominant ESBLs type (100%), while those for TEM and SHV-types were 85% and 80% respectively. Four genotype patterns were detected (CTX-M, TEM+CTX-M, SHV+CTX-M and SHV+TEM+CTX-M). The genotype SHV+TEM+CTX-M, was the predominant (70%), followed by the genotype TEM +CTX-M combination (15%). The occurrences of the genotypes, CTX-M and SHV+CTX-M were 5% and 10% respectively. Conclusion: This study found a high rate of Phenotypic ESBL production (74.5%) among the isolates with multidrug resistance, CTX-M as the predominant ESBLs type (100%) and combination of SHV+TEM+CTX-M as the predominant genotype (70%).

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“…In Togo, results from previous studies revealed the presence of beta-lactamase gene CTX-M1 (95.73%), TEM (82.31%) and SHV (45.12%) in ESBL-producing E. coli and Klebsiella spp. The production of ESBL was associated with high co-resistance to fluoroquinolone (93% for ciprofloxacin), aminoglycosides (76.36% for gentamicin) and trimethoprim/sulfamethoxazole (95.65%) [15, 16]. This high level of multidrug resistance suggests acquisition of plasmid-mediated antibiotic resistance factors in these strains.…”

Section: Introductionmentioning

confidence: 99%

Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo

Salah

Soubeïga

Ouattara

et al. 2019

Antimicrob Resist Infect Control

110

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Background Qnr genes are known to confer a low-level resistance to fluoroquinolone in Enterobacteriaceae . They are often found on the same resistance plasmids as extended spectrum β-lactamase (ESBL) and constitute the most common antibiotic resistance mechanism. This study aimed to detect the presence of qn r genes in ESBL -producing E. coli and Klebsiella spp . Methods From May 2013 to July 2015, 91 E. coli and 64 Klebsiella spp. strains with phenotypic resistance to quinolone were collected from several specimens and analyzed for the detection of qnrA, qnrB, qnrS genes and the β-lactamase resistance genes ( blaCTX-M , blaTEM , blaSHV ) using simplex and multiplex PCR. Results In the present study, 107 (69%; 61 E. coli and 46 Klebsiella spp. ) of 155 bacterial strains tested were found harboring at least one qnr gene consisting of 74 (47.74%) qnrB , 73 (47.10%) qnrS and 4 (2.58%) qnrA . Of the 107 strains encoding qnr genes, 102, 96 and 52 carried CTX-M1 , TEM and SHV type ESBL respectively. Conclusion This study identified quinolone resistance ( qnr ) gene in ESBL -producing E. coli and Klebsiella spp. in Togo. These finding which suggest a possible resistance to quinolone are of high interest for better management of patients and control of antimicrobial resistance in Togo. Electronic supplementary material The online version of this article (10.1186/s13756-019-0552-0) contains supplementary material, which is available to authorized users.

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First Detection of Resistance Genes Encoding Extended Spectrum Beta-Lactamase Producing Escherichia coli at Lome, Togo

Cited by 10 publications

References 45 publications

Multi-Drug Resistant (MDR) Bacterial Isolates on Close Contact Surfaces and Health Care Workers in Intensive Care Units of a Tertiary Hospital in Bulawayo, Zimbabwe

Multi-Drug Resistant (MDR) Bacterial Isolates on Close Contact Surfaces and Health Care Workers in Intensive Care Units of a Tertiary Hospital in Bulawayo, Zimbabwe

Prevalence and molecular characterization of extended spectrum beta-lactamase producing uropathogenic Escherichia coli in a tertiary health care facility in Anambra State, Nigeria

Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo

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