First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid

Mei, Federico; Bonifazi, Martina; Menzo, Stefano; Berardino, Alessandro Di Marco; Sediari, Michele; Paolini, Luca; Re, Alessia; Gonnelli, Francesca; Duranti, Claudia; Grilli, Martina; Vennarucci, Giacomo Spurio; Latini, Maria Agnese; Zuccatosta, Lina; Gasparini, Stefano

doi:10.1016/j.chest.2020.05.583

Cited by 35 publications

(39 citation statements)

References 11 publications

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“…To address this issue, Merindol et al (2020) developed an improved RT-PCR based method for direct SARS-CoV-2 detection, which does not require RNA extraction step and is therefore significantly faster. Another recently developed RT-PCR based method of SARS-CoV-2 detection is reverse transcription loop-mediated isothermal amplification (RT-LAMP) ( Mei et al, 2020 ). Furthermore, a colorimetric detection assay was adapted to RT-LAMP method to increase its throughput.…”

Section: Virus Detection By G-quadruplex Biosensormentioning

confidence: 99%

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Juhas

Zhang

2020

Biosensors and Bioelectronics

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G-quadruplex is a non-canonical nucleic acid structure formed by the folding of guanine rich DNA or RNA. The conformation and function of G-quadruplex are determined by a number of factors, including the number and polarity of nucleotide strands, the type of cations and the binding targets. Recent studies led to the discovery of additional advantageous attributes of G-quadruplex with the potential to be used in novel biosensors, such as improved ligand binding and unique folding properties. G-quadruplex based biosensor can detect various substances, such as metal ions, organic macromolecules, proteins and nucleic acids with improved affinity and specificity compared to standard biosensors. The recently developed G-quadruplex based biosensors include electrochemical and optical biosensors. A novel G-quadruplex based biosensors also show better performance and broader applications in the detection of a wide spectrum of pathogens, including SARS-CoV-2, the causative agent of COVID-19 disease. This review highlights the latest developments in the field of G-quadruplex based biosensors, with particular focus on the G-quadruplex sequences and recent applications and the potential of G-quadruplex based biosensors in SARS-CoV-2 detection.

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Section: Virus Detection By G-quadruplex Biosensormentioning

confidence: 99%

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Juhas

Zhang

2020

Biosensors and Bioelectronics

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“…And both, heart failure and pulmonary embolism [ 17 ], in addition to respiratory co-infections [ 18 ], are well-recognized etiologies of pleural effusions. Despite the fact that a certain number of patients may have pleural effusions derived from the preceding causes, SARS-CoV-2 undoubtedly gives rise to fluid formation since it has been detected by reverse-transcriptase polymerase chain reaction in pleural fluid specimens [ 19 , 20 ].…”

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confidence: 99%

Pleural diseases and COVID-19:ubi fumus, ibi ignis

Porcel

2020

Eur Respir J

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More than 45 000 articles in the PubMed database and around 3200 studies registered in ClinicalTrials.gov, of which greater than half are clinical trials, is the result of ongoing and relentless research into the global pandemic nature of an acute respiratory disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which made its initial appearance in December 2019 in China. As of August 28, the total confirmed cases of coronavirus disease 2019 (COVID-19) surpass 24.5 million people, with over 830 000 global deaths [1]. An estimated 40% to 45% of persons infected with SARS-CoV-2 will remain asymptomatic, but they can transmit the virus to others for an extended period, perhaps longer than 14 days [2]. The primary presentation of symptomatic infection is that of an influenza-like illness or viral pneumonia, with about 20% of these patients developing severe or critical manifestations [3].

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“…Bennet D et al [20] described case report of 61 year old renal transplant patient, who had shown presence of SARS-CoV-2 in plural uid by RT-PCR. SARS-CoV-2 was also detected from pleural uid in study done by Malik MI et al [21] (1 case), Mei F et al [22] (1 case), Baek MS et al [23] (1 case) and Turriziani O et al [24] (3 out of 8 cases). As pleural mesothelial cell expresses DPP4 (CD26) receptor, which plays a role for entry of a virus inside the cell, pleura is target for SARS-CoV-2.…”

Section: Resultsmentioning

confidence: 98%

Detection of SARS-CoV-2 in Different Body Fluids Using RT-PCR – An Autopsy Based Study

Kyada¹,

Trangadia²,

Vadgama³

et al. 2020

Preprint

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World is in mid of dreaded pandemic – Covid-19 caused by SARS-CoV-2. To curb it tremendous efforts has been put worldwide to enhance scientific knowledge related to SARS-CoV-2. In this study, we collected postmortem samples of tracheal swab, vitreous humor, pleural fluid, CSF and pericardial fluid by conducting complete autopsy on 24 patients with known SARS-CoV-2 infection at department of forensic medicine, P.D.U. Government College, Rajkot (India). RT-PCR was used to detect SARS-CoV-2 from these samples. SARS-CoV-2 was detected from tracheal swab in 54.55% cases and from pleural fluid in 13.34% cases. SARS-CoV-2 wasn’t detected from any sample of CSF, vitreous humor and pericardial fluid. Positive and negative cases for postmortem tracheal swabs were analyzed to find out of its relationship with duration of Covid-19 illness. No significant relationship was found between detectability of SARS-CoV-2 in postmortem samples of tracheal swab and duration of Covid-19 illness.

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First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid

Cited by 35 publications

References 11 publications

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Pleural diseases and COVID-19:ubi fumus, ibi ignis

Detection of SARS-CoV-2 in Different Body Fluids Using RT-PCR – An Autopsy Based Study

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