First Detection of TEM-116 and SHV-75 Producing Enterobacteria Isolated from Two Ivorian Teaching Hospitals: Case of Abidjan and Bouake

Toty, Abalé Anatole; Guessennd, Nathalie; Akoua‐Koffi, Chantal; Otokore, D. A.; Meex, Cécile; Mbengue, G. V.; Djaman, Allico Joseph; Dosso, Mireille; Galleni, Moreno

doi:10.20546/ijcmas.2016.505.001

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The sensitivity test of bacterial strains to the compound isolated from EC 321 was carried out by the technique of diffusion in agar medium. In Petri dishes containing the agar medium of Mueller Hinton Agar, a suspension of the bacterial strains at a concentration of 10 6 CFU/ml was inoculated by spreading using a swab [24] . Antibiogram discs were impregnated with 15 μl of the molecular solution of the compound isolated at the concentration of 0.25 and 0.5 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

“…In Petri dishes containing the agar medium of Mueller Hinton Agar, a suspension of the bacterial strains at a concentration of 10 6 CFU/ml was inoculated by spreading using a swab. [24] Antibiogram discs were impregnated with 15 μl of the molecular solution of the compound isolated at the concentration of 0.25 and 0.5 mg/ml. Positive and negative control controls were carried respectively with a reference antibiotic ampicillin at 30 μg/disk and DMSO diluted to 1/10.…”

Section: Antibacterial Activity Of Secalonic Acid a Isolated From D S...mentioning

confidence: 99%

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Diversity of Endophytic Fungi Isolated from the Bark of Ceiba pentandra (L.) Gaertn., (Bombacaceae) and Antibacterial Potential of Secalonic Acid A Produced by Diaporthe searlei EC 321

Dominique,

Alex,

Christiane

et al. 2023

Chemistry & Biodiversity

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The objective of this study was to study the diversity of endophytic fungi isolated from Ceiba pentandra and to isolate their bioactive chemical compounds. The methodology used during this study consisted in isolating endophytic fungi from the bark of C. pentandra on Potato Agar. The isolates obtained were identified on the basis of the ITS regions of their ribosomal DNA. Antibacterial screening of the mycelium of endophytic fungi isolated was evaluated against multidrug‐resistant E. coli and S. aureus strains. This screening led to the selection of isolates EC 321 and EC 28 for their ability to effectively inhibit the growth of the bacterial strains tested. EC 321 was grown and fermented on rice medium. Secondary metabolites were extracted with ethyl acetate. From the crude extract, secalonic acid A was isolated and identified by chromatographic and NMR. The in vitro activity of secalonic acid A against the growth of multiresistant bacterial strains was evaluated. Secalonic acid A was active against all multidrug‐resistant bacterial strains E. coli 942, E. coli 4814, S. aureus 931, S. aureus 934, S. aureus MRSA 1872 and K. pneumonia 815 with respective MICs of 18.75; 18.75; 18.75; 4.7; 37.5 and 37.5 μg/mL.

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Section: Methodsmentioning

confidence: 99%

Section: Antibacterial Activity Of Secalonic Acid a Isolated From D S...mentioning

confidence: 99%

Diversity of Endophytic Fungi Isolated from the Bark of Ceiba pentandra (L.) Gaertn., (Bombacaceae) and Antibacterial Potential of Secalonic Acid A Produced by Diaporthe searlei EC 321

Dominique,

Alex,

Christiane

et al. 2023

Chemistry & Biodiversity

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“…It is widely used in traditional medicine for the treatment of anemia, gastroenteritis, nephroses and malaria [7,8]. Recently, our team showed that this plant had antibacterial effect on Enterobacteriaceae producing β-lactamases with broad spectrum [9]. The aim of this study was to determine the inhibitory effect of the hydro-ethanolic extract of H. madagascariensis on pure β-lactamases.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity of β-lactamases by Hydro-Ethanolic Extracts of Harungana madagascariensis, a Plant of the Ivorian Pharmacopoeia

Anatole

Edwige

Bertin

et al. 2020

AJRB

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Infection due to multi-resistant bacteria is a public health concern. Unfortunately, the prospect of developing new antibiotics seem not to be on the horizon. Faced with this impasse, medicinal plants could be an alternative for the development of new molecules. The aim of this study was to determine the inhibitory effect of H. madagascariensis extracts on β-lactamases. Extraction of bioactive compounds from trunk barks and leaves of the plant was done in an ethanol-water mixture (70:30). Anti-β-lactamase activity was evaluated by spectrophotometry after the removal of tannins and a phytochemical screening was used to identify the groups of compounds. Concentrations inhibiting 50% of enzyme activity were 0.005±0.001 mg/mL (CTX-M-15), 0.01±0.001 mg/mL (P99) and 0.027±0.009 mg/mL (NDM-1) for bark extracts and 0.704 mg/mL for leaves extracts. Phytochemical screening revealed the presence of flavonoids in bark extracts. The ethanolic extracts of the trunk bark exert a good inhibitory activity on CTX-M-15, P99 and NDM-1 β-lactamases and this activity could be attributed to the presence of the flavonoids. Further studies by bio-guided fractionation of the ethanolic extracts of the bark could yield fractions with high inhibitory potential of β-lactamases.

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The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt

et al. 2021

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First Detection of TEM-116 and SHV-75 Producing Enterobacteria Isolated from Two Ivorian Teaching Hospitals: Case of Abidjan and Bouake

Cited by 4 publications

References 25 publications

Diversity of Endophytic Fungi Isolated from the Bark of Ceiba pentandra (L.) Gaertn., (Bombacaceae) and Antibacterial Potential of Secalonic Acid A Produced by Diaporthe searlei EC 321

Diversity of Endophytic Fungi Isolated from the Bark of Ceiba pentandra (L.) Gaertn., (Bombacaceae) and Antibacterial Potential of Secalonic Acid A Produced by Diaporthe searlei EC 321

Inhibitory Activity of β-lactamases by Hydro-Ethanolic Extracts of Harungana madagascariensis, a Plant of the Ivorian Pharmacopoeia

The alarming association between antibiotic resistance and reduced susceptibility to biocides in nosocomial MRSA isolates from two regional hospitals in Egypt

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