First field-based evidence of association of Proteus mirabilis causing large scale mortality in Indian major carp farming

Pattanayak, S; Kumar, Pasim Rajesh; Sahoo, Manoj Kumar; Paul, Anirban; Sahoo, P.K.

doi:10.1016/j.aquaculture.2018.06.006

Cited by 11 publications

(9 citation statements)

References 45 publications

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“…Hemolysin hpmA is often found more than hlyA in P. mirabilis uropathogenic [17], which may justify the absence of the hlyA gene and the presence of hpmA in both strains of our study. The presence of the hmpA and zapA genes has also been reported in P. mirabilis, causing large-scale fish mortality [11]. The gene ireA is responsible for encoding a siderophore receptor, which is considered an important virulence factor in obtaining host Fe 3+ for the maintenance of bacterial metabolism during the infectious process [12].…”

Section: Discussionmentioning

confidence: 98%

“…P. mirabilis is classified as an opportunistic pathogen, because it can eventually cause significant and persistent infections in humans such as wounds, eye infections, and gastrointestinal and urinary tract infections [9]. Studies on the association of P. mirabilis with infections in animals are scarce, but urinary tract infections in dogs and cats have been reported [10], and in fish, causing a high mortality rate [11].…”

Section: Introductionmentioning

confidence: 99%

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Proteus mirabilis causing cellulitis in broiler chickens

et al. 2020

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Given the need to understand the virulence profile of Proteus mirabilis isolates from cellulitis in broiler chickens and their ability to cause lesions, the present study aimed to characterize genotypically and phenotypically the virulence profiles of two strains of P. mirabilis isolated from cellulitis in broilers, as well as to evaluate their ability to experimentally reproduce the lesions in vivo. The strain with the highest virulence potential (LBUEL-A33) possessed mrpA, pmfA, ucaA, atfA (fimbriae), zapA, ptA (proteases), hpmA (hemolysin), and ireA (siderophore) genes, formed a very strong biofilm, and expressed the pattern of aggregative adhesion and cytotoxicity in Vero cells. The strain with the lowest virulence potential (LBUEL-A34) did not present the pmfA and ucaA genes, but expressed the pattern of aggregative adhesion, formed a strong biofilm, and did not show cytotoxicity. Both strains developed cellulitis in an animal model within 24 h post-inoculation (PI), and the degree of lesions was not significantly altered up to 120 h PI. The LBUEL-A33 strain was also inoculated in combination with an avian pathogenic Escherichia coli (APEC 046), and the lesions showed no significant changes from the individual inoculation of these two strains. Histological analysis showed that the LBUEL-A33 strain developed characteristic cellulitis lesions. Thus, both strains of P. mirabilis isolated in our study have several virulence factors and the ability to develop cellulitis in broilers.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Proteus mirabilis causing cellulitis in broiler chickens

et al. 2020

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“…In addition, P . mirabilis , a conditional pathogen [ 73 ], was also shown to be the most abundant isolate in the control group, while it was rarely observed in other woody forage groups, suggesting that the woody forage had a specific antibacterial activity [ 74 , 75 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, whether Bacillus megaterium can also improve the growth of tilapia or even other fish is a question that is worth elucidating. In addition, P. mirabilis, a conditional pathogen [73], was also shown to be the most abundant isolate in the control group, while it was rarely observed in other woody forage groups, suggesting that the woody forage had a specific antibacterial activity [74,75].…”

Section: Plos Onementioning

confidence: 99%

Effects of woody forages on biodiversity and bioactivity of aerobic culturable gut bacteria of tilapia (Oreochromis niloticus)

Chen

Liu

et al. 2020

PLoS ONE

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The present study investigated the effects of four woody forages (Moringa oleifera Lam (MOL), fermented MOL, Folium mori (FM) and fermented FM) on biodiversity and bioactivity of aerobic culturable gut bacteria of tilapia (Oreochromis niloticus) by a traditional culturedependent method. A total of 133 aerobic culturable isolates were recovered and identified from the gut of tilapia, belonging to 35 species of 12 genera in three bacterial phyla (Firmicutes, Actinobacteria and Proteobacteria). Among them, 6 bacterial isolates of Bacillus baekryungensis, Bacillus marisflavi, Bacillus pumilus, Bacillus methylotrophicus, Proteus mirabilis and Pseudomonas taiwanensis were isolated from all the five experimental groups. The Bray-Curtis analysis showed that the bacterial communities among the five groups displayed obvious differences. In addition, this result of bioactivity showed that approximate 43% of the aerobic culturable gut bacteria of tilapia displayed a distinct anti-bacterial activity against at least one of four fish pathogens Streptococcus agalactiae, Streptococcus iniae, Micrococcus luteus and Vibrio parahemolyticus. Furthermore, Bacillus amyloliquefaciens and Streptomyces rutgersensis displayed strong activity against all four indicator bacteria. These results contribute to our understanding of the intestinal bacterial diversity of tilapia when fed with woody forages and how certain antimicrobial bacteria flourished under such diets. This can aid in the further exploitation of new diets and probiotic sources in aquaculture.

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“…Currently, the opportunistic pathogen P. mirabilis is found to be an emerging threat to animals. It was reported that P. mirabilis could cause large-scale mortality of fish without association with any other pathogens [ 8 ]. In addition, P. mirabilis infections have been reported in birds with reproductive failure [ 9 ], cattle and fowl with diarrhea [ 10 , 11 ] and dogs with chronic otitis externa [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Carbapenem Resistance of Proteus mirabilis Strains Isolated from Foxes, Raccoons and Minks in China

Hao

Cao

et al. 2022

Biology

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Proteus mirabilis, an opportunistic pathogen, is found to be an emerging threat to both animals and humans for a variety of infections. However, the characteristics of P. mirabilis infections from foxes, raccoons and minks remain unclear. In this context, we identified the antibiotic resistance genes and virulence genes of P. mirabilis isolates from foxes, raccoons and minks in China. Most isolates showed resistance to florfenicol (90.57%), trimethoprim-sulfamethoxazole (73.58%), and imipenem (71.70%). A total of 73.58% of isolates were resistant to antibiotics from at least three or more classes, and were categorized as multi-drug resistant. A total of 33.33% of the isolates were resistant to antibiotics from seven classes. The most prevalent resistant were sul1 (94.34%), followed by floR, blaTEM, aac(6’)Ib-cr and blaOXA-1 with the detection rate of 88.68%, 83.02%, 71.70% and 60.38%, respectively. Among the 51 P. mirabilis isolates that were resistant to beta-lactam antibiotics, all isolates carried at least one beta-lactam gene. In addition, blaNDM and blaOXA-24 genes were firstly reported in carbapenem-resistant P. mirabilis isolates from foxes, raccoons and minks. All isolates exhibited the virulence genes ureC, zapA, pmfA, atfA and mrpA. P. mirabilis isolates carrying all detected 10 virulence genes from different animal species showed different lethal abilities in a G. mellonella larvae model. More importantly, the profiles of antibiotic resistance genes of isolates from fur animals and the environment were generally similar, and phylogenetic analysis showed that the P. mirabilis isolates from farm environment samples may have close relatedness with that from animals.

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First field-based evidence of association of Proteus mirabilis causing large scale mortality in Indian major carp farming

Cited by 11 publications

References 45 publications

Proteus mirabilis causing cellulitis in broiler chickens

Proteus mirabilis causing cellulitis in broiler chickens

Effects of woody forages on biodiversity and bioactivity of aerobic culturable gut bacteria of tilapia (Oreochromis niloticus)

Detection of Carbapenem Resistance of Proteus mirabilis Strains Isolated from Foxes, Raccoons and Minks in China

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