First genotyping of Enterocytozoon bieneusi in plateau pikas (Ochotona curzoniae) from the Qinghai Plateau, Northwest China

Liu, Xuehan; Du, Shou-Chao; Yang, Xuefeng; Xia, Xiaojing; An, Zhixing; Qi, Meng

doi:10.1007/s11259-021-09824-0

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…(Wu et al, 2021), Cryptosporidium spp. (Zhang et al, 2018), Toxoplasma gondii (Zhang et al, 2013), Enterocytozoon bieneusi (Liu et al, 2021). However, the presence of S. stercoraria in plateau pikas has never been reported before.…”

Section: Introductionmentioning

confidence: 95%

Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: genome descriptions and phylogenetic evolution

Zhang,

Chen,

Meng

et al. 2024

Parasitology

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As a member of the Scathophagidae family, Scathophaga stercoraria (S. stercoraria) is widely distributed globally and is closely associated with animal feces. It is also a species of great interest to many scientific studies. However, its phylogenetic relationships are poorly understood. In this study, S. stercoraria was found in plateau pikas for the first time. The potential cause of its presence in the plateau pikas was discussed and it was speculated that the presence of S. stercoraria was related to the yak feces. In addition, two nuclear genes (18SrDNA and 28SrDNA), one mitochondrial gene (COI), and the complete mitochondrial genome of S. stercoraria was sequenced.Phylogenetic trees constructed based on 13 Protein coding genes (13PCGs), 18S and 28S rDNA showed that S. stercoraria is closely related to the Calliphoridae family; phylogenetic results based on COI suggest that within the family Scathophagidae, S. stercoraria is more closely related to the genus Leptopa, Micropselapha, Parallelomma, and Americina. Divergence times estimated using the COI gene suggest that the divergence formation of the genus Scathophaga is closely related to changes in biogeographic scenarios and potentially driven by a combination of uplift of the Qinghai-Tibetan Plateau (QTP) and dramatic climate changes. These results provide valuable information for further studies on the phylogeny and differentiation of the Scathophaga genus in the future.

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Section: Introductionmentioning

confidence: 95%

Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: genome descriptions and phylogenetic evolution

Zhang,

Chen,

Meng

et al. 2024

Parasitology

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“…In these sampling areas, there were also some other wild animals, such as pikas and marmots. In accordance with relevant research, 33 fresh fecal samples of plateau pikas were collected from the Qinghai Plateau area, and 5 (15.2%, 5/33) isolates were positive for E. bieneusi ( Liu et al, 2021 ). For further study, we could collect more samples from more animal species to analyze the infection rate of E. bieneusi and its prevalent genotypes.…”

Section: Discussionmentioning

confidence: 93%

“…To date, limited data about E. bieneusi infections in Tibetan sheep, yak, pika, wild Himalayan marmots, and Alashan ground squirrels had been reported ( Liu et al, 2021 ; Xu et al, 2020 ; Zhang et al, 2018 ), but no information about infection with E. bieneusi from Przewalski's gazelle, blue sheep and wild birds has been reported. The study was conducted to detect E. bieneusi in domesticated livestock and wildlife around Qinghai Lake to enrich the epidemiological and genotyping data, evaluate the mutual transmission risk between domesticated livestock and wildlife, and assess the possibility of infection risk to humans.…”

Section: Introductionmentioning

confidence: 99%

First report on the molecular detection of Enterocytozoon bieneusi in livestock and wildlife around Qinghai Lake in the Qinghai-Tibetan Plateau area, China

Jian¹,

Zhang²,

Wang³

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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“…Additionally, comprehensive phylogenetic analysis has classified these ITS genotypes into 14 distinct genetic clusters, named Group 1–14 [ 25 , 39 ]. The genotypes in Group 1 are further divided into nine subgroups (1a–1i) and infect both humans and animals, posing a zoonotic threat [ 19 ]. The genotypes in the remaining genetic groups (2–14) were primarily isolated from specific hosts, suggesting strong host specificity and a limited public health threat [ 15 , 25 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and genotyping of Enterocytozoon bieneusi in flying squirrels (Trogopterus xanthipes) from China

Liu,

Zhang,

et al. 2024

Parasite

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Enterocytozoon bieneusi is an obligate intracellular microsporidian parasite with a worldwide distribution. As a zoonotic pathogen, E. bieneusi can infect a wide range of wildlife hosts through the fecal-oral route. Although the feces of flying squirrels (Trogopterus xanthipes) are considered a traditional Chinese medicine (as “faeces trogopterori”), no literature is available on E. bieneusi infection in flying squirrels to date. In this study, a total of 340 fresh flying squirrel fecal specimens from two captive populations were collected in Pingdingshan city, China, to detect the prevalence of E. bieneusi and assess their zoonotic potential. By nested PCR amplification of the ITS gene, six specimens tested positive, with positive samples from each farm, with an overall low infection rate of 1.8%. The ITS sequences revealed three genotypes, including known genotype D and two novel genotypes, HNFS01 and HNFS02. Genotype HNFS01 was the most prevalent (4/6, 66.7%). Phylogenetic analysis showed that all genotypes clustered into zoonotic Group 1, with the novel genotypes clustering into different subgroups. To our knowledge, this is the first report of E. bieneusi infection in flying squirrels, suggesting that flying squirrels could act as a potential reservoir and zoonotic threat for E. bieneusi transmission to humans in China.

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First genotyping of Enterocytozoon bieneusi in plateau pikas (Ochotona curzoniae) from the Qinghai Plateau, Northwest China

Cited by 4 publications

References 20 publications

Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: genome descriptions and phylogenetic evolution

Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: genome descriptions and phylogenetic evolution

First report on the molecular detection of Enterocytozoon bieneusi in livestock and wildlife around Qinghai Lake in the Qinghai-Tibetan Plateau area, China

Occurrence and genotyping of Enterocytozoon bieneusi in flying squirrels (Trogopterus xanthipes) from China

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