First histopathological study in kidneys of rodents naturally infected with Leptospira pathogenic species from Yucatan, Mexico

Torres–Castro, Marco; Guillermo-Cordero, Leonardo; Hernández-Betancourt, Silvia F.; Gutiérrez-Ruiz, E. J.; Agudelo‐Flórez, Piedad; Sanchez, Ronald Guillermo Peláez; Zavala-Castro, Jorge; Puerto, Fernando I.

doi:10.1016/j.apjtm.2016.01.018

Cited by 14 publications

(13 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The histological changes described in the present study are similar to those found by Carvalho and collaborators [11] in sheep and by Torres-Castro et al, [47] in rodents, in relation to the evidence of interstitial nephritis. In leptospirosis, inflammatory infiltrate is a primary alteration during acute renal injury and can be caused by direct damage by leptospires to the host tissue or by the presence of leptospiral antigen, initiating a renal immune response [36].…”

Section: Discussionsupporting

confidence: 92%

Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse

et al. 2019

View full text Add to dashboard Cite

The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti- Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L . interrogans . The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.

show abstract

Section: Discussionsupporting

confidence: 92%

Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Los infiltrados celulares inflamatorios presentes se clasificaron según su porcentaje de afección en tejido en: leve (<30%), moderado (30-50%) y severo (>50%), según lo establecido por Torres-Castro et al (2016). Los patrones de distribución fueron reportados según lo propuesto por Trigo & Valero (2004): focal: un solo sitio afectado; multifocal: varios sitios afectados, separados por tejido sano y normal, y zonal: un área total afectada.…”

Section: Estudio Histológicounclassified

Histological lesions associated to the possible infection by Trypanosoma cruzi (Chagas, 1909) in hearts of synanthropic rodents captured in Yucatan, Mexico

Torres–Castro¹,

Hernández-Betancourt²,

Puerto

et al. 2016

An Biol

Self Cite

View full text Add to dashboard Cite

Trypanosoma cruzi es el agente causal de la tripanosomiasis americana, padecimiento endémico en México y cuyos reservorios más importantes son los roedores. T. cruzi refiere tropismo al corazón, ocasionando lesiones y colonizando con nidos con amastigotes; por lo que el estudio histológico de este tejido ayuda al diagnóstico. Se capturaron 262 roedores en Yucatán, México; todos considerados en el análisis microscópico, en el cual se observaron lesiones compatibles a la infección por T. cruzi: infiltrado celular inflamatorio (86.7%), degeneración de fibras cardiacas (6.8%) y pericarditis (6.3%). Asimismo, fue posible identificar nidos con amastigotes (2.2%). Este es el primer estudio histológico realizado en roedores sinantrópicos posiblemente infectados por T. cruzi en Yucatán, México.Trypanosoma cruzi is known as the causal agent of American trypanosomiasis, endemic zoonotic disease in Mexico, whose most important reservoirs are rodents. T. cruzi refers tropism to heart, causing various lesions and colonizing with nests of amastigotes; so the histological examination of this tissue helps for diagnosis. A total of 262 individuals were captured in Yucatan, Mexico; all considered in the microscopic analysis, in which different lesions consistent with T. cruzi infection were identified: inflammatory cell infiltration (86.7%), degeneration of cardiac fibers (6.8%) and pericarditis (6.3%). It was also possible to identify several nests with amastigotes (2.2%). This is the first histological study made in synanthropic rodents probably infected by T. cruzi in Yucatan, Mexico.

show abstract

“…This work is the first approximation to the study of Leptospira in rodents in the northern part of the state of Veracruz. Besides, it represents the first molecular confirmation of the presence of L. borgspetersenii in rodents, particularly in Mus musculus, in México (Espinosa-Martínez et al 2015;Torres-Castro et al 2016Panti-May et al 2017). The reference serovar of L. borgspetersenii isolated from M. musculus is Ballum, which was detected in the past century in Europe (Yager et al 1953).…”

Section: Discussionmentioning

confidence: 87%

“…In México, few studies have been conducted to detect Leptospira sp. in rodents, mostly in the states of Tamaulipas, Campeche, and Yucatán (Méndez et al 2013;Espinosa-Martínez et al 2015;Torres-Castro et al 2016; Panti-May et in each locality. The rodents captured were removed from traps, identified, and processed for kidney sample collection following biosafety standards, under collection license FAUT-0250 granted by the Secretariat of Environment and Natural Resources (Semarnat).…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for the presence of Leptospira borgpetersenii in synanthropic rodents in the Nautla region, Veracruz, México

Gutiérrez-Molina

Cruz‐Romero

Romero-Salas

et al. 2019

Therya

View full text Add to dashboard Cite

The genus Leptospira encompasses ten species of spirochetes capable of infecting mammals, particularly rodents. In México, studies focused on the detection of Leptospira sp. in rodents are scarce, all of them restricted to three states of the Gulf of México. For this reason, this work aimed to identify the diversity of Leptospira species associated with synanthropic rodents in Veracruz, a state where leptospirosis is endemic. Rodents were sampled with Sherman traps placed in 10 Production Units across the Nautla region. Animals were euthanized and their kidneys removed. Subsequently, a 474-bp segment of the outer membrane protein LipL32, present in all pathogenic species, was amplified and sequenced. Sequences were compared vs. reference using the BLAST algorithm: a phylogenetic reconstruction was carried out using the Maximum Likelihood method. In addition, the prevalence of infection in each Production Unit was estimated. Twenty eight rodents of a single species (Mus musculus) were caught. Leptospira DNA was detected in 17 samples (62.9 %, CI 95 % 42.3 to 80.6) from seven localities in the Nautla region. The sequences recovered exhibited 99-100% identity to each other and 99 % identity with Leptospira borgspetersenii sequences deposited in GenBank. This study confirms the presence of L. borgspetersenii in rodents, particularly in M. musculus, in México. This study increases the inventory of pathogenic leptospires for the state of Veracruz to three species.El género Leptospira engloba 10 especies de espiroquetas capaces de infectar mamíferos, particularmente roedores. En México se han realizado escasos estudios para la detección de Leptospira sp. en roedores, todos ellos restringidos a tres estados del Golfo de México. Por tal motivo el objetivo del presente trabajo fue identificar la diversidad de leptospiras en roedores sinantrópicas de Veracruz, un estado endémico de leptospirosis. Para la colecta de roedores, se colocaron trampas tipo Sherman en 10 unidades de producción de la región Nautla. Los animales se sacrificaron y se obtuvieron los riñones. Posteriormente se amplificó y secuenció un segmento de 474 pb de la proteína exterior de membrana LipL32 presente en las leptospiras patógenas. Posteriormente se compararon las secuencias con las de referencia mediante el uso del algoritmo BLAST y se realizó una reconstrucción filogenética mediante el método de Máxima Verosimilitud. Adicionalmente se obtuvieron las prevalencias de la infección por unidad de producción. Se colectaron 28 roedores de una única especie (Mus musculus). Se detectó la presencia de ADN de Leptospira en 17 muestras (62.9 %; IC 95 % 42.3 a 80.6) procedentes de siete localidades de la región Nautla. Las secuencias recuperadas exhibieron una similitud del 99-100 % entre si y una identidad del 99 % con secuencias de referencia de Leptospira borgspetersenii depositadas en GenBank. Este estudio confirma la presencia de L. borgspetersenii en roedores y en particular con M. musculus en México. Este estudio incrementa a tres especies el inventario ...

show abstract

First histopathological study in kidneys of rodents naturally infected with Leptospira pathogenic species from Yucatan, Mexico

Abstract: The histopathological lesions are present in the kidneys, plus the results of the polymerase chain reaction confirm that these rodents are true carriers of Leptospira spp.

Cited by 14 publications

References 15 publications

Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse

Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse

Histological lesions associated to the possible infection by Trypanosoma cruzi (Chagas, 1909) in hearts of synanthropic rodents captured in Yucatan, Mexico

Molecular evidence for the presence of Leptospira borgpetersenii in synanthropic rodents in the Nautla region, Veracruz, México

Contact Info

Product

Resources

About