First identification of Cryptosporidium parvum subtype IIaA20G1R1 in water buffalos (Bubalus bubalis)

Martins, Thais Agostinho; Seixas, Mércia de; Brito, Danilo Rodrigues Barros; Martins, Felippe Danyel Cardoso; Cardim, Sérgio Tosi; Melo, Priscilla; Guterres, Sonália Ferreira da Paixão; Patrício, Edjanio Gaspar; Garcia, João Luís

doi:10.1016/j.rvsc.2018.02.002

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…Reports of subtype IIaA20G1R1 in humans are rare. However, the IIaA20G1R1 subtype was seen in water buffalo in Brazil [ 32 ] and in cattle in Serbia and Montenegro [ 33 ], Sweden [ 34 ], and Brazil [ 35 ]. The second most common subtpes of C .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

Woreta

Degarege²,

Adamu³

et al. 2021

PLoS ONE

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Data on the distribution and genotype of Cryptosporidium species is limited in Ethiopia. This study examined the presence and genetic diversity of Cryptosporidium species circulating in Ethiopian human population. Stool samples collected from patients who visited rural (n = 94) and urban (n = 93) health centers in Wurgissa and Hawassa district, respectively, were examined for the presence of Cryptosporidium spp. using microscopy, nested PCR and real-time PCR. To detect infection with PCR, analysis of 18S ribosomal RNA was performed. Subtyping was performed by sequencing a fragment of GP60 gene. The overall prevalence of infection was 46% (n = 86) by microscope and PCR. When 48 (out of 86) PCR positive samples were genotyped, two species were identified: C. parvum (n = 40) and C. hominis (n = 8). When 15 of the 40 C. parvum isolates were subtyped, zoonotic subtypes of IIaA14G1R1 (n = 1), IIaA15G2R1 (n = 1), IIaA16G1R1 (n = 2), IIaA16G3R1 (n = 2), IIaA17G1R1 (n = 1), IIaA19G1R1 (n = 1), IIaA20G1R1 (n = 3), IIaA22G1R1 (n = 1), IIaA22G2R1 (n = 1), IIdA23G1 (n = 1) and IIdA24G1 (n = 1) were identified. When 6 of the 8 C. hominis isolates were subtyped, subtypes IaA20 (n = 5), and IdA21(n = 1) were identified. This study suggests that C. parvum and C. hominis are causes of cryptosporidiosis in human in the Wurgissa district and Hawassa in Ethiopia. Zoonotic transmission might be the main route of transmission.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

Woreta

Degarege²,

Adamu³

et al. 2021

PLoS ONE

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“…With the growing economic impact of buffalo, the prevalence of infectious diseases affecting this species and the mechanisms of host immune response are in the spotlight of veterinary research on farm animals (Panigrahi et al, 2016;El-Halawany et al, 2017, Hornok et al, 2018Martins et al, 2018;Paradiso et al, 2018). Still, little is known about the immune system of healthy water buffaloes.…”

Section: Introductionmentioning

confidence: 99%

Blood lymphocyte subpopulations in healthy water buffaloes (Bubalus bubalis, Mediterranean lineage): Reference intervals and influence of age and reproductive history

Martini

Bernardi

Russo

et al. 2019

Veterinary Immunology and Immunopathology

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“…ryanae infection in 2 samples, whereas the 3rd isolate was unidenti ed but related genetically to the bovine C. ryanae and C. bovis as well as the ovine C. xiaoi. C ryanae is the most common Cryptosporidium species reported worldwide in buffaloes 41,42 , but also C. parvum, and C. bovis [41][42][43][44] . In Romania, C. parvum was reported in cattle, and as the predominant species in lambs 40,45,46 .…”

Section: Discussionmentioning

confidence: 99%

Intestinal Parasites of Buffalo Calves from Romania: Molecular Characterization of Cryptoporidium spp. and Giardia Duodenalis, and the first Report of Eimeria Bareillyi

Bărburaș

Cozma

Ionică

et al. 2021

Preprint

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Buffaloes represent an important economic resource for several regions of the world including Romania; however, no reports on parasitic infections in buffaloes from Romania are available. In the present study, we examined for the gastrointestinal parasites 104 fecal samples bimonthly collected from 38 buffalo calves (2–11 weeks old) from household rearing systems in Romania. All samples were tested using the saturated salt flotation, McMaster and modified Ziehl-Nielsen staining methods. PCR coupled with isolates sequencing methods were used to identify the Giardia duodenalis assemblages and Cryptosporidium species. Overall, 33 out of 38 examined buffalo calves were infected with different gastrointestinal parasites; 16 had single infections and 17 had mixed infections with 2 or 3 parasites. Eimeria species (32/38; 84.2%) was the most prevalent parasite; 8 species were identified according to the oocyst morphology including the pathogenic E. bareillyi which detected for the first time in buffaloes from Romania. Toxocara vitulorum (11/38; 36.8%) and Strongyloides papillosus (6/38; 15.8%) were also detected. Cryptosporidium spp. were found in 4 (10.5%) buffalo calves; 2 of them were molecularly identified as C. ryanae and another one was clustered in the same clade with C. ryanae, C. bovis, and C. xiaoi. Giardia duodenalis assemblage E was also molecularly detected in a single (2.6%) buffalo calf. The presence of other buffaloes in the same barn was identified as a risk factor for infection with T. vitulorum. Our results indicate extensive parasitic infections in buffalo calves from Northwestern Romania and underline the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.

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First identification of Cryptosporidium parvum subtype IIaA20G1R1 in water buffalos (Bubalus bubalis)

Cited by 7 publications

References 20 publications

Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

Blood lymphocyte subpopulations in healthy water buffaloes (Bubalus bubalis, Mediterranean lineage): Reference intervals and influence of age and reproductive history

Intestinal Parasites of Buffalo Calves from Romania: Molecular Characterization of Cryptoporidium spp. and Giardia Duodenalis, and the first Report of Eimeria Bareillyi

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