First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina

Travería, Gabriel Eduardo; Zumárraga, Martín José; Etchechoury, Ignacio; Romanó, Maria; Cataldi, Angel; Pinedo, María Fiorella Alvarado; Pavlík, I.; Pribylova, Radka; Romero, Jaime

doi:10.1590/s1517-83822013005000066

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…The totality of the isolates (100%) were genotyped and grouped into five different INMV previously reported patterns (24,30 3%). This technique when applied to MAP showed a lower discriminatory index (HGDI: 0•6984) than that obtained for MAH isolates.…”

Section: Map Isolatesmentioning

confidence: 99%

“…Molecular techniques based on the polymorphisms present in the length of fragments obtained by restriction enzymes [Restriction fragment length polymorphism (RFLP)] of specific IS (IS 900 , IS 1245 , and IS 1311 ) have been used to differentiate MAC subspecies and even strains belonging to the same subspecies [10, 23]. This method, however, is very laborious and requires large amounts of genomic DNA seldom obtained from MAP [24].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity ofMycobacterium aviumcomplex strains isolated in Argentina by MIRU-VNTR

Imperiale

Moyano

Giulio³

et al. 2017

Epidemiol. Infect.

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Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.

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Section: Map Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity ofMycobacterium aviumcomplex strains isolated in Argentina by MIRU-VNTR

Imperiale

Moyano

Giulio³

et al. 2017

Epidemiol. Infect.

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“…This represents a situation comparable to a low to medium within-flock prevalence for OJD in Australia, where OJD has been extensively studied [35]. No studies on within-flock prevalence of OJD caused by S-type MAP are available from SA, where the only report of S type MAP described an individual case of OJD [11]. More extensive epidemiological studies are needed to investigate the prevalence and distribution of OJD in the region.…”

Section: Discussionmentioning

confidence: 97%

“…Although this type is commonly found in sheep in Australia [21] and Europe [7], nearly all reports on molecular typing of MAP from SA sheep, cattle, goats, deer, alpacas, guanacos, hares, bovine milk, and environmental samples, including Argentina, Brazil, Chile, Colombia and Venezuela [2,3,10,[26][27][28][29][30][31], and the Caribbean [32], have been of the C type. MAP S-type has only been described once in SA (Argentina) in a single case of OJD in a Milchschaf ram [11]. Although the genotyping method we used targeting IS1311 is one of the most widely applied methodologies for MAP typing, along with mycobacterial interspersed repetitive units-variable numbers of tandem repeats (MINRU-VNTR), these methods have limitations when compared to genome-wide single nucleotide polymorphism (SNP) and phylogenetic analyses [7].…”

Section: Discussionmentioning

confidence: 99%

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Mycobacterium paratuberculosis sheep type strain in Uruguay: Evidence for a wider geographic distribution in South America

Giannitti

Fraga

Caffarena

et al. 2018

J Infect Dev Ctries

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Johne’s disease (JD) is an economically important disease of ruminants caused by Mycobacterium avium paratuberculosis (MAP), which also infects other species including humans. Two major MAP strain types are currently recognized: sheep (S) and cattle (C) types. Information on JD prevalence and MAP types infecting small ruminants in South America is limited, and all but one of the MAP types reported from this region are of the C type. This study describes clinicopathological, molecular and microbiological findings in 11 cases of JD caused by a type S MAP strain, and estimated true within-flock prevalence in a ~735-sheep operation in Uruguay. Postmortem examination and histology (hematoxylin-eosin and Ziehl-Neelsen stains) of samples from 41 selected sheep revealed lymphohistiocytic/granulomatous enteritis and mesenteric lymphadenitis in 11 animals, with moderate/severe multibacillary lesions in 6 clinical cases, and minimal/mild paucibacillary lesions in 5 sub-clinical cases. Immunohistochemistry using an antibody against Mycobacterium bovis that cross-reacts with MAP (2 cases), and transmission electron microscopy (1 case), revealed myriads of intrahistiocytic mycobacteria. MAP was isolated in one case and detected by PCR in 6 cases. The S type of MAP was identified using a multiplex PCR that distinguishes between S and C types, and PCR-REA. The estimated true within-flock prevalence was ≤ 2.3%. This represents the first communication on within-flock prevalence of JD associated with a type S MAP strain in South America and the second documentation of this strain in the subcontinent. Additional studies are required to better understand the molecular epidemiology of the different MAP types in the region.

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First molecular epidemiological study of Mycobacterium avium subsp. paratuberculosis in cattle and buffalo from different regions of Brazil

Albuquerque

Melo

Brito

et al. 2018

Trop Anim Health Prod

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Paratuberculosis is an incurable disease in ruminants with great worldwide economic impact, caused by Mycobacterium avium subsp. paratuberculosis (MAP). The objective of this study was to carry out a study of the molecular epidemiology of the MAP using the restriction enzyme analysis (REA) technique of IS1311 MAP region in biological samples of feces, intestinal tissue, and mesenteric lymph nodes of cattle and buffaloes from six Brazilian states. In total, 109 samples of feces and tissues of cattle and buffaloes were collected from animal paratuberculosis suspected. Twenty-five samples were positive in the detection of the DNA of the IS900 region of MAP and it was possible to type 18 strains in the analysis of the region IS1311, being 100% of them identified as belonging to subtype Bison MAP strain. This is the first epidemiological molecular study of MAP in Brazil. The results indicate that paratuberculosis is widespread in cattle and in buffaloes in several regions of Brazil, and the subtype Bison MAP strain was the only one identified in the samples analyzed in this study, demonstrating the similarity between the strains from different states tested. These results provide the necessary support for the implementation of paratuberculosis control strategies in cattle and buffaloes in Brazil.

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First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina

Cited by 5 publications

References 19 publications

Genetic diversity ofMycobacterium aviumcomplex strains isolated in Argentina by MIRU-VNTR

Genetic diversity ofMycobacterium aviumcomplex strains isolated in Argentina by MIRU-VNTR

Mycobacterium paratuberculosis sheep type strain in Uruguay: Evidence for a wider geographic distribution in South America

First molecular epidemiological study of Mycobacterium avium subsp. paratuberculosis in cattle and buffalo from different regions of Brazil

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