First isolation, identification and characterisation of Tenacibaculum maritimum in Norway, isolated from diseased farmed sea lice cleaner fish Cyclopterus lumpus L

Småge, Sverre Bang; Frisch, Kathleen Marie; Brevik, Øyvind Jakobsen; Watanabe, K.; Nylund, Are

doi:10.1016/j.aquaculture.2016.06.030

Cited by 53 publications

(46 citation statements)

References 37 publications

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“…The T. maritimum isolates described in this study belonged to two distinct STs (STCan1 and STCan2) different from all other previously published STs (Habib et al., ), and the MLSA shows that the Western Canadian strains are most closely related with strain NLF‐15 isolated from lumpsuckers in Norway (Småge et al., ) and strain Ch‐2402 isolated from Atlantic salmon in Chile (Apablaza et al., ). These four strains are grouped together in subgroup C (designated C in Habib et al.…”

Section: Discussionmentioning

confidence: 44%

“…The phylogenetic relationship of the two Tenacibaculum maritimum Canadian strains, the Norwegian T. maritimum strain NLF ‐15 (Småge et al., ), the Chilean T. maritimum strain Ch‐2402 (Apablaza et al., ), as well as a single strain from each of the 47 T. maritimum ST s (identified by their ST number) (Habib et al., ) based on the concatenated HK gene sequences. The Bayesian analysis was performed on 5811 bp.…”

Section: Resultsmentioning

confidence: 99%

“…()) with three strains from Spain (salmonids and turbot, Scophthalmus maximus , L.) and two from Tasmania (Atlantic salmon and striped trumpeter, Latris lineata , Forster). Strains NLF‐15 and Ch‐2402 were isolated from fish reared in waters of approximately 12°C and 14 °C, respectively (Apablaza et al., ; Småge et al., ), which is in the same range as the Canadian isolates (Table ). Temperatures from the other strains in subgroup C are unknown to the authors of this study, but thought to be in the same temperature range (9–16°C), which is in the lower range of T. maritimum growth (Suzuki et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…The first HK gene sequence alignment included concatenated sequences of the Canadian strains found in this study and 19 type strains in genus Tenacibaculum . The second HK gene sequence alignment included concatenated sequences of the Canadian strains found in this study (Table ), the Norwegian T. maritimum strain NLF‐15 isolated from lumpsuckers, Cyclopterus lumpus (L.) (Småge, Frisch, Brevik, Watanabe, & Nylund, ), the Chilean T. maritimum strain Ch‐2402 isolated from Atlantic salmon (Apablaza et al., ), as well as a single strain from each of the 47 T. maritimum STs (identified by their ST number) described in Habib et al. ().…”

Section: Methodsmentioning

confidence: 99%

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Genotyping of Tenacibaculum maritimum isolates from farmed Atlantic salmon in Western Canada

Frisch

Småge

Brevik³

et al. 2017

Journal of Fish Diseases

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Section: Discussionmentioning

confidence: 44%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Genotyping of Tenacibaculum maritimum isolates from farmed Atlantic salmon in Western Canada

Frisch

Småge

Brevik³

et al. 2017

Journal of Fish Diseases

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show abstract

“…; Småge et al . ), with outbreaks occurring both in land‐based rearing facilities and at sea when transferred into salmon pens.…”

Section: Introductionmentioning

confidence: 99%

Piscirickettsia salmonis infection in cultured lumpfish (Cyclopterus lumpus L.)

Marcos-López¹,

Ruane

Scholz³

et al. 2017

Journal of Fish Diseases

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A Piscirickettsia salmonis infection was diagnosed in lumpfish (Cyclopterus lumpus L.) juveniles held in a marine research facility on the west coast of Ireland. The main clinical signs and pathology included marked ascites, severe multifocal liver necrosis and severe diffuse inflammation and necrosis of the exocrine pancreas and peri-pancreatic adipose tissue. Numerous Piscirickettsia-like organisms were observed by histopathology in the affected organs, and the bacterial species was characterized by molecular analysis. Sequencing of the partial 16S rDNA gene and internal transcribed spacer region showed the lumpfish sequences to be closely related to previously identified Atlantic salmon (Salmo salar L.) sequences from Ireland. To the authors' knowledge, this is the first detection of P. salmonis in lumpfish worldwide. The infection is considered potentially significant in terms of lumpfish health and biosecurity.

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Full‐length 16S rRNA gene classification of Atlantic salmon bacteria and effects of using different 16S variable regions on community structure analysis

2019

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Understanding fish‐microbial relationships may be of great value for fish producers as fish growth, development and welfare are influenced by the microbial community associated with the rearing systems and fish surfaces. Accurate methods to generate and analyze these microbial communities would be an important tool to help improve understanding of microbial effects in the industry. In this study, we performed taxonomic classification and determination of operational taxonomic units on Atlantic salmon microbiota by taking advantage of full‐length 16S rRNA gene sequences. Skin mucus was dominated by the genera Flavobacterium and Psychrobacter. Intestinal samples were dominated by the genera Carnobacterium, Aeromonas, Mycoplasma and by sequences assigned to the order Clostridiales. Applying Sanger sequencing on the full‐length bacterial 16S rRNA gene from the pool of 46 isolates obtained in this study showed a clear assignment of the PacBio full‐length bacterial 16S rRNA gene sequences down to the genus level. One of the bottlenecks in comparing microbial profiles is that different studies use different 16S rRNA gene regions. Comparisons of sequence assignments between full‐length and in silico derived variable 16S rRNA gene regions showed different microbial profiles with variable effects between phylogenetic groups and taxonomic ranks.

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First isolation, identification and characterisation of Tenacibaculum maritimum in Norway, isolated from diseased farmed sea lice cleaner fish Cyclopterus lumpus L

Cited by 53 publications

References 37 publications

Genotyping of Tenacibaculum maritimum isolates from farmed Atlantic salmon in Western Canada

Genotyping of Tenacibaculum maritimum isolates from farmed Atlantic salmon in Western Canada

Piscirickettsia salmonis infection in cultured lumpfish (Cyclopterus lumpus L.)

Full‐length 16S rRNA gene classification of Atlantic salmon bacteria and effects of using different 16S variable regions on community structure analysis

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