First Molecular Characterization of the Partial Coat Protein and 3’utr Genes Related to Dasheen Mosaic Virus on Flamingo Flowers (Anthurium Species) in the Mediterranean Coast of Turkey

Abstract

Observations were carried out on Anthurium sp growing at Mediterranean region (Adana, Mersin and Antalya provinces) of Turkey in 2015-2017. During the studies virus-like symptoms in plants; systemic chlorotic mosaic and irregular uniformity on leaf and petals were recognized. Eleven of 73 samples showed positive response with the Potyvirus group specific antibodies and their results were also confirmed by RT-PCR. The 327 bp products via using the Potyvirus-specific primers, MJ1 and MJ2 were run in 1.5% agarose gel. Further amplifications were made with the 3'UTR and 3'end of the coat protein of the viral genome. RT-PCR analysis using the primer MJ1 and M4T resulted in an amplification of 719 bp amplicon. Direct sequencing of RT-PCR products and BLAST results have showed that there were two clades between 96-99% among the Mediterranean coast DsMV isolates. The data are available in Genebank with accession Number MK758088/Antalya; MK758087/Yenice). DsMV infection on Anthurium sp, was determined serologically and molecularly. To our knowledge, this is the first molecular record describing the occurrence, distribution and genome organisation of DsMV isolates that have been infecting Flamingo flowers at the Mediterranean coast of Turkey. Findings may have shown an evidence about uncontrolled distribution from Northern corner to Southern side.