First Molecular Detection of Borrelia afzelii in Clinical Samples in Korea

Choi, Yeon‐Joo; Han, Seunghoon; Park, Jin-Mi; Lee, Kyung-Min; Lee, Eun-Mi; Lee, Seung–Hyun; Song, Hyeon-Je; Koh, Young-Sang; Lee, Keun-Wha; Jang, Won‐Jong; Park, Kyung-Hee

doi:10.1111/j.1348-0421.2007.tb04015.x

Cited by 17 publications

(20 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The purified PCR products were confirmed by direct sequencing using the CEQ 2000 Dye Terminator Cycle Sequencing method with the Quick Start kit (Beckman Coulter, Inc., Fullerton, CA, U.S.A.) and the CEQ 2000 DNA analysis system (Beckman Coulter, Inc.) according to the manufacturer's instructions. PCR targeting borrelia rrf(5S)-rrl(23S) intergenic spacer gene and RFLP were then performed as previously described [3,23]. The reaction mixture contained 1.5 µl of DNA (approximately 100 ng) sample as template in a total reaction volume of 30 µl.…”

confidence: 99%

Prevalence of Lyme Borrelia in Ixodes persulcatus Ticks from an Area with a Confirmed Case of Lyme Disease

et al. 2013

View full text Add to dashboard Cite

ABSTRACT. In this study, the prevalence of Borrelia infections in Ixodes ticks from a site in Hokkaido, Japan, with confirmed cases of Lyme disease was determined by a PCR method capable of detecting and differentiating between strains of pathogenic Borrelia, with particular emphasis on Borrelia garinii (B. garinii) and Borrelia afzelli (B. afzelli), using tick-derived DNA extracts as template. A total of 338 ticks, inclusive of 284 Ixodes persulcatus (I. persulcatus), were collected by flagging vegetation in mid-spring. Ninety-eight (34.5%) of I. persulcatus tested positive for Borrelia species DNA, whereas the overall prevalence of Borrelia species in Ixodes ovatus and Haemaphysalis longicornis ticks was 19.5 and 7.7%, respectively. PCR-RFLP and sequence analysis of Borrelia rrf(5S)-rrl(23S) intergenic spacer DNA amplicons indicated that they originated from three different Borrelia species namely, B. garinii, B. afzelii and B. japonica. Among the I. persulcatus species, which is a known vector of human borreliosis, 86 were mono-infected with B. garinii, 2 ticks were mono-infected with B. afzelii and whereas 12 ticks had dual infections. Most significant, 11 of the I. persulcatus ticks were coinfected with Anaplasma phagocytophilum and B. garinii. The difference between the number of obtained and expected co-infections was significant (χ 2 =4.32, P=0.038).

show abstract

confidence: 99%

Prevalence of Lyme Borrelia in Ixodes persulcatus Ticks from an Area with a Confirmed Case of Lyme Disease

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Since then, the number of cases has gradually increased; 23 cases have been reported in 2019, including 11 imported cases [11]. To date, various molecular and serological studies have identified the pathogenic cause of LD in ticks and animals including wild rodents, migratory birds, water deer, and dogs [12][13][14][15][16][17][18][19][20]. Borrelia afzelii and B. garinii were first identified in I. persulcatus and wild rodents, respectively, from the Gangwon province.…”

Section: Introductionmentioning

confidence: 99%

Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea

Kim

et al. 2020

Pathogens

View full text Add to dashboard Cite

: Lyme disease is a tick-borne zoonotic disease caused by Borrelia burgdorferi sensu lato (s. l.) via transmission cycles involving competent tick vectors and vertebrate reservoirs. Here, we determined the prevalence and distribution of Borrelia genospecies in 738 ticks of at least three species from wild rodents in nine regions of the Republic of Korea (ROK). Ticks were analyzed using nested PCR targeting partial flagellin B gene sequences, followed by sequence analysis. The prevalence of Borrelia infection was 33.6%, and the most common genospecies were B. afzelii (62.5%), B. valaisiana (31.9%), B. yangtzensis (2.4%), B. garinii (1.6%), and B. tanukii (1.6%). Borrelia afzelii was found in all regions except Jeju Island; this predominant genospecies was found in the northern and central sampling regions. Borrelia valaisiana, B. yangtzensis, and B. tanukii were found only in the southern regions with B. valaisiana being the most common, whereas B. yangtzensis and B. tanukii were only found on Jeju Island. Our study is the first to describe the nationwide prevalence of B. burgdorferi s. l. in ticks from wild rodents in the ROK. Continuous surveillance in ticks, animals, humans, and different regions is required to avoid disease distribution and possible transmission to humans in the ROK.

show abstract

“…Subsequent technological advances enabled the development of diagnostic molecular tests, which were shown to be more sensitive than cultures . Molecular epidemiology of human isolates of Lyme Borrelia (LB) has clarified the global prevalence of the B. burgdorferi sensu lato complex, which now also includes Europe ( B. burgdorferi sensu stricto (ss), Borrelia afzelii and Borrelia garinii ), eastern Russia ( B. burgdorferi ss, B. afzelii and B. garinii ), China ( B. afzelii and B. garinii ), Japan ( B. afzelii and B. garinii ), South Korea ( B. afzelii ) and Taiwan ( B. burgdorferi ss, B. afzelii and B. garinii ) and extends into South America ( B. burgdorferi ss, Borrelia chilensis and B. garinii ) …”

Section: Diagnostics/pathogenesismentioning

confidence: 99%

Lyme disease: why the controversy?

Beaman

2016

Internal Medicine Journal

View full text Add to dashboard Cite

Some Australians have become convinced of the existence of locally acquired Lyme disease (LD). The history of LD, since its recognition in the early 1970s, is reviewed as a model for investigative approaches to unknown syndromes. Australian Management Guidelines for LD include the requirement for diagnostic testing by National Association of Testing Authorities-accredited laboratories using Therapeutic Goods Administrationlicensed tests, which result in the efficient diagnosis of LD in overseas travellers. Despite this, patients who have not left Australia pay many thousands of dollars for nonspecialist consultations and testing at overseas laboratories. Unproven long-term therapy with multiple antibiotics has resulted in serious complications, including allergies, line sepsis, pancreatitis and pseudomembranous colitis. Studies have shown that LD vectors are not found in Australia, and Lyme Borrelia has not been found in Australian vectors, animals or patients with autochthonous illnesses. I propose that (i) A noncontroversial name for the chronic syndrome should be adopted, 'Australian Multisystem Disorder'. (ii) Research funding should enable the development of a consensus case definition and studies of the epidemiology of this syndrome with laboratory investigations to identify an aetiology and surrogate markers of disease. Prospective, randomised treatment studies could then be undertaken using ethical protocols.

show abstract

First Molecular Detection of Borrelia afzelii in Clinical Samples in Korea

Cited by 17 publications

References 32 publications

Prevalence of Lyme Borrelia in <i>Ixodes persulcatus</i> Ticks from an Area with a Confirmed Case of Lyme Disease

Prevalence of Lyme Borrelia in <i>Ixodes persulcatus</i> Ticks from an Area with a Confirmed Case of Lyme Disease

Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea

Lyme disease: why the controversy?

Contact Info

Product

Resources

About