First Molecular Detection of Rickettsia Felis in Fleas From Algeria

Bitam, Idir; Parola, Philippe; Cruz, Katharina Dittmar de la; Matsumoto, Koutaro; Baziz, Belkacem; Rolain, Jean‐Marc; Belkaid, M; Raoult, Didier

doi:10.4269/ajtmh.2006.74.532

Cited by 76 publications

(77 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Surprisingly, R. felis may be present in rat fl eas (X. cheopis) as demonstrated here and in a recent report from Indonesia (9). The prevalence of R. felis ranges from 5% to 45.8% for large collections of fl eas, sometimes up to 100% when small collections are tested (8,10,11), and is often higher when compared with the prevalence of R. typhi, as in our study. R. felis has also been detected in Anomiopsyllus nudata collected on white-throated woodrats (Neotoma albigula) (12).…”

Section: Discussionsupporting

confidence: 52%

Rickettsia typhiandR.felisin Rat Fleas (Xenopsylla cheopis), Oahu, Hawaii

Eremeeva

Warashina²,

Sturgeon³

et al. 2008

Emerg. Infect. Dis.

View full text Add to dashboard Cite

Rickettsia typhi (prevalence 1.9%) and R. felis (prevalence 24.8%) DNA were detected in rat fl eas (Xenopsylla cheopis) collected from mice on Oahu Island, Hawaii. The low prevalence of R. typhi on Oahu suggests that R. felis may be a more common cause of rickettsiosis than R. typhi in Hawaii.M urine typhus is a febrile zoonotic disease caused by Rickettsia typhi. The classic view is that R. typhi circulates among rats (Rattus rattus or R. norvegicus) and rat fl eas (Xenopsylla cheopis) (1,2), although other rodents and their ectoparasites have been implicated in maintenance of R. typhi in nature. Humans become infected when they visit disease-endemic areas infested with rats and acquire infection by inhalation or by self-inoculating infected fl eas or fl ea feces into skin.The most recent outbreak of murine typhus in Hawaii occurred in 2002 with 47 cases reported on 5 islands (3). Concomitantly, an increase occurred in the mouse population on the island of Maui, which reported 35 human cases. Peak occurrence of murine typhus in Hawaii was in 1944 with 186 cases reported, of which 80% occurred on the island of Oahu (4). Previous serologic surveys in Hawaii have identifi ed antibodies reactive with R. typhi antigen in the Polynesian rat (R. exulans), black rat (R. rattus), Norway rat (R. norvegicus), and house mouse (Mus musculus) (3,4). The Indian mongoose (Herpestes auropunctatus) was also identifi ed as a potential reservoir; however, its role has not yet been evaluated. We conducted a molecular survey of fl eas in Oahu to characterize the prevalence and identity of rickettsiae in Hawaii. The StudyM. musculus mice were collected during rodent population studies in the leeward and southeast parts of Oahu during the summers of 2004, and 2007. Fleas were combed from each animal, identifi ed as X. cheopis by using standard taxonomic keys, and kept frozen at -70°C until they were sent to the Centers for Disease Control and Prevention (Atlanta, GA, USA) for further analyses. Mice were humanely killed; only brains were removed and frozen.DNA was isolated from each fl ea by using the Biomek 2000 Laboratory Automation workstation (Beckman, Fullerton, CA, USA) and reagents from the Wizard Prep kit (Promega, Madison, WI, USA) (5). DNA from 20 mg of mouse brain tissue was isolated by using the QiaAmp Mini kit (QIAGEN, Valencia, CA, USA).

show abstract

Section: Discussionsupporting

confidence: 52%

Rickettsia typhiandR.felisin Rat Fleas (Xenopsylla cheopis), Oahu, Hawaii

Eremeeva

Warashina²,

Sturgeon³

et al. 2008

Emerg. Infect. Dis.

View full text Add to dashboard Cite

show abstract

“…and R. felis have been detected previously in fl eas within northern and sub-Saharan Africa (10). The presence of Bartonella spp.…”

Section: Bartonella Spp and Rickettsia Felis In Fleas Democratic Rementioning

confidence: 96%

“…Serum, pharyngeal, and fecal swab samples were collected and stored as described previously (10). An ELISA was developed to detect antibodies to the NiV nucleocapsid (N) and attachment glycoprotein (G) proteins.…”

Section: Antibodies To Nipah or Nipah-like Viruses In Bats Chinamentioning

confidence: 99%

Bartonellaspp. andRickettsia felisin Fleas, Democratic Republic of Congo

Sackal¹,

Laudisoit²,

Kosoy³

et al. 2008

Emerg. Infect. Dis.

View full text Add to dashboard Cite

“…During the necropsy, our findings revealed that the most prevalent helminth of long-eared hedgehogs was P. clausa. This heteroxenous nematode is distributed (23,25,26,27). In the present study, M. erinacei (Anoplocephalinae) is first reported in Iran.…”

Section: Discussionmentioning

confidence: 94%

“…The hedgehogs potentially could be infected with some Rickettsia pathogens (19,20,21). Rickettsiae massiliae has been detected in European hedgehogs from France (22) as well as from African and desert hedgehogs from Algeria (11,23). R. massiliae is a causative agent of spotted fever disease in humans (24).…”

Section: Discussionmentioning

confidence: 99%

Endoparasites of the Long-Eared Hedgehog (Hemiechinus auritus) in Zabol District, Southeast Iran

Zolfaghari¹,

Nabavi²

2016

TurkiyeParazitolDerg

View full text Add to dashboard Cite

Objective: The long-eared hedgehog (Hemiechinus auritus) is a nocturnal animal living in Central and Southeast Iran. However, there are few studies concerning endoparasites, some of which are zoonotic, of the hedgehogs in the north and northwest of Iran. The aim of the present study is to investigate endoparasites in long-eared hedgehogs, living in Zabol district, Southeast Iran. Materials and Methods: Stool and blood samples collected from 50 hedgehogs (35 males and 15 females) that were trapped alive were examined with Clayton-Lane flotation and Giemsa staining methods. Furthermore, 10 road-killed hedgehog carcasses were necropsied. The adult parasites were collected and identified under a light microscope. Results: Spirurida eggs in the stool samples and Anaplasma inclusion bodies in red blood cells were determined in 32% and 52% of the samples, respectively. Physaloptera clausa, Mathevotaenia erinacei, Nephridiacanthus major, and Moniliformis moniliformis were identified in the necropsy. Conclusion: To the best of our knowledge, ours is the first study concerning endoparasites of long-eared hedgehogs in Iran. Furthermore, M. erinacei was for the first time reported as a parasitic fauna in Iran. (Turkiye Parazitol Derg 2016; 40: 37- (Turkiye Parazitol Derg 2016; 40: 37-41)

show abstract

First Molecular Detection of Rickettsia Felis in Fleas From Algeria

Cited by 76 publications

References 27 publications

Rickettsia typhiandR.felisin Rat Fleas (Xenopsylla cheopis), Oahu, Hawaii

Rickettsia typhiandR.felisin Rat Fleas (Xenopsylla cheopis), Oahu, Hawaii

Bartonellaspp. andRickettsia felisin Fleas, Democratic Republic of Congo

Endoparasites of the Long-Eared Hedgehog (Hemiechinus auritus) in Zabol District, Southeast Iran

Contact Info

Product

Resources

About