First molecular detection of tick-borne pathogens in dogs from Jiangxi, China

Zheng, Weiqing; Liu, Mingming; Moumouni, Paul Franck Adjou; Liu, Xiaoqing; Efstratiou, Artemis; Liu, Zhanbin; Liu, Yangqing; Tao, Huiying; Guo, Huanping; Wang, Guanbo; Gao, Yang; Li, Zifen; Ringo, Aaron Edmund; Jirapattharasate, Charoonluk; Chen, Haiying; Xuan, Xuenan

doi:10.1292/jvms.16-0484

Cited by 36 publications

(26 citation statements)

References 31 publications

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“…B. gibsoni is the most widespread species in China; it has been reported in Shandong, Jiangsu, Anhui, Shanghai, Zhejinag, Nanjing, Jiangxi, and Guangxi. B. canis vogeli is the other species reported in dogs, whose epidemic areas include Jiangxi and Gansu (Wei et al, 2012 ; Yao et al, 2014 ; Niu et al, 2017 ; Zheng et al, 2017 ). Very recently, Niu et al reported T. sinensis in pet dogs in Gansu, representing the first report of T. sinensis in dogs (Niu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Yao et al reported that B. gibsoni is the main species responsible for canine babesiosis in Nanjing (Yao et al, 2014 ). In Jiangxi, the rates of positivity for B. canis vogeli and B. gibsoni are 4.94 and 2.47%, respectively, as tested by species-specific PCR (Zheng et al, 2017 ). In 2017, Niu et al first reported the identification of Theileria sinensis in pet dogs from Gansu province of China, providing the first report of T. sinensis in dogs worldwide (Niu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

Miao

et al. 2017

Front. Microbiol.

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Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection. Parasites were observed in 10 blood samples via microscopic examination, whereas there were 14 Babesia gibsoni-positive RLB tests. Phylogenetic analysis was performed after the 18S rRNA and ITS gene sequences from the 14 positive samples were cloned and sequenced. The results confirmed the existence of B. gibsoni in this area. This is the first molecular report of canine babesiosis in pet dogs from Wuhan, China. Pet dogs are companion animals, and the prevalence of babesiosis will be of concern in daily life. This study will help veterinarians better understand the prevalence of canine babesiosis and provide a guide for disease control in pet dogs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

Miao

et al. 2017

Front. Microbiol.

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“…infections in dogs continue to increase worldwide, primarily in many developing countries but also in several developed countries. Previous epidemiological studies regarding Babesia infection in dogs and ticks demonstrated that four species of Babesia were circulating in China, and B. gibsoni was the predominant pathogen causing babesiosis in the central, southern and eastern regions of China [ 18 – 20 , 22 , 23 , 38 ]. However, little information concerning the epidemiology of Babesia spp.…”

Section: Discussionmentioning

confidence: 99%

“…As the most prevalent Babesia species, B. gibsoni is responsible for canine babesiosis in the central, southern and eastern regions of China, as well as Taiwan [15][16][17][18][19][20]. Babesia vogeli has been identified in dogs in Gansu, Jiangxi, Guangdong and Hunan provinces [21,22] and in ticks in Henan [23]. Babesia canis has been reported in dogs in Henan [24] and various 18S rRNA gene sequences of B. canis have been identified in dogs and ticks in Hunan and Qinghai provinces, respectively.…”

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confidence: 99%

Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China

Guo

Xie

et al. 2020

Parasites Vectors

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Background: Several members of genus Babesia are important pathogens causing babesiosis in dogs. In China, at least five Babesia species have been described in dogs or ticks. This study sought to determine the prevalence and molecular characteristics of various Babesia spp. in dogs in cities in Shaanxi Province in China, including Xi'an and Hanzhong. Methods: A total of 371 blood samples were collected from pet dogs presenting to veterinary clinics in the cities of Xi'an and Hanzhong in Shaanxi, China. Babesia spp. DNA was detected via amplification of partial 18S rRNA genes by semi-nested PCR. Almost full-length 18S rRNA, ITS, partial TRAP and complete cytb genes were recovered for analysis of the genetic characteristics and relationships with known isolates. Results: A single species, Babesia gibsoni, was identified in dogs in Xi'an and Hanzhong. Consistently, B. gibsoni was also detected in 14 ticks collected from positive dogs. Sequence similarities and phylogenetic analysis suggested that the isolates identified herein showed a closer genetic relationship with isolates from East Asian countries rather than India, Bangladesh, or the USA. Sequence analysis based on tandem repeat analysis of the TRAP gene further revealed that specific haplotypes were circulating in both Xi'an and Hanzhong, with no specific regionality. In addition, 10.9% of all isolates with atovaquone (ATV)-resistance were identified because of M121I mutation in the deduced cytb protein. Conclusions: This study revealed a high prevalence rate of Babesia infection. Babesia gibsoni was the only Babesia species identified in cases of canine babesiosis in the cities of Xi'an and Hanzhong cities in Shaanxi, China. In addition, the TRAP gene presented high genetic diversity across isolates. Such information is useful for elucidating the epidemiological characteristics of canine babesiosis, as well as the overall genetic diversity of Babesia spp. circulating in dog populations in Shaanxi Province.

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“…To date, at least three protozoan ( Theileria, Babesia and Hepatozoon ) and five bacterial ( Anaplasma, Ehrlichia, Rickettsia, Coxiella and Bartonella ) tick-borne genera have been reported in domestic dogs around the globe (Beck et al 2009 ; Brown et al 2006 ; Buhariwalla et al 1996 ; Camacho et al 2001 ; Conrad et al 1991 ; Kaewkong et al 2014 ; Kamani et al 2013 ; Levin et al 2012 ; Mokhtar et al 2013 ; Yabsley et al 2008 ). In Jiangxi Province, mid-eastern China, Babesia canis vogeli and Babesia gibsoni were molecularly detected in 780 dog ticks (749 Rhipicephalus sanguineus , 16 Haemaphysalis campaulata and 15 Haemaphysalis verticalis ), while all sampled dog ticks were negative for rickettsial agents (Zheng et al 2017 ). In Xinjiang Uygur Autonomous Region (XUAR), northwestern China, rickettsial agents were prevalent in ticks infesting both domestic animals and wildlife (Guo et al 2015 , 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of spotted fever group rickettsiae in ticks parasitizing pet dogs in Shihezi City, northwestern China

et al. 2019

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A total of 178 adult ticks were collected from 32 pet dogs from five veterinary clinics in Shihezi City, Xinjiang Uygur Autonomous Region (XUAR), northwestern China. All the ticks were identified by comprehensive morphological and genetic analyses, and rickettsiae were detected by seven Rickettsia-specific genetic markers in the ticks. The ticks collected were identified as Rhipicephalus sanguineus sensu lato. Twenty-one of the 178 samples (11.8%) were positive for rickettsiae. Among these, in 13 (61.9%) samples Candidatus R. barbariae were identified, in five (23.8%) samples R. massiliae, and in three (14.3%) samples R. conorii. This study indicates that more attention should be paid to rickettsial infection in pet dogs and their ticks, because the latter may pose an epidemiological risk for tick-borne transmission of rickettsiae to human beings.Electronic supplementary materialThe online version of this article (10.1007/s10493-018-00337-1) contains supplementary material, which is available to authorized users.

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First molecular detection of tick-borne pathogens in dogs from Jiangxi, China

Cited by 36 publications

References 31 publications

First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China

Molecular detection of spotted fever group rickettsiae in ticks parasitizing pet dogs in Shihezi City, northwestern China

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