First molecular evidence of Coxiella burnetii in patients with atypical pneumonia, India

Gangoliya, Shefali Raj; Kumar, Sanjay; Alam, Syed Imteyaz; Devi, Gayathri; Guchhait, Partha

doi:10.1099/jmm.0.000232

Cited by 16 publications

(11 citation statements)

References 11 publications

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“…Therefore, it is logical to assume that the infection by C. burnetii has remained grossly under-diagnosed in the Indian population largely due to the lack of facilities for culture and advanced diagnostic assays for this infectious agent. Only two references have shown the application of molecular techniques for diagnosis of Q fever in humans in recent years [12,13]. To the best of our knowledge, there is no report of acute Q fever among patients with acute febrile illness in India employing molecular tools.…”

mentioning

confidence: 99%

First genetic evidence of Coxiella burnetii in cases presenting with acute febrile illness, India

Kumar

Gangoliya

Alam

et al. 2017

Journal of Medical Microbiology

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mentioning

confidence: 99%

First genetic evidence of Coxiella burnetii in cases presenting with acute febrile illness, India

Kumar

Gangoliya

Alam

et al. 2017

Journal of Medical Microbiology

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“…By the application of molecular diagnosis using Trans-PCR, Indian researchers reported prevalence of Q fever 21.6% of human abortions, 11.05% of domestic animals with reproductive disorders, and 2.8% of patients with atypical pneumonia [4,5,34]. Our study points to the fact that Trans 3 and 4 are sensitive than Trans 1 and 2, as it is a short fragment in the most conserved region of C. burnetii DNA.…”

Section: Resultsmentioning

confidence: 63%

“…There is not much of a progress in India in the molecular diagnosis of coxiellosis/Q fever, except for few reports from Uttar Pradesh, Karnataka, and Tamil Nadu [4-7,31,34]. By the application of molecular diagnosis using Trans-PCR, Indian researchers reported prevalence of Q fever 21.6% of human abortions, 11.05% of domestic animals with reproductive disorders, and 2.8% of patients with atypical pneumonia [4,5,34].…”

Section: Resultsmentioning

confidence: 99%

“…Our study points to the fact that Trans 3 and 4 are sensitive than Trans 1 and 2, as it is a short fragment in the most conserved region of C. burnetii DNA. Use of 243 bp or even smaller 70 bp amplicon [34] could perhaps pick up more positive cases in humans as well as domestic livestock. Das et al .…”

Section: Resultsmentioning

confidence: 99%

“…No serosurvey reports on coxiellosis in Indian animals appeared after the eighties, until the recent report of coxiellosis in small ruminants of Puducherry in 2014 [32]. Evidence of animal and human abortions, neonatal septicemia, endocarditis, and atypical pneumonia due to C. burnetii based on immunofluroescence test/polymerase chain reaction (PCR) are recorded in recent Indian literature [4,5,33,34]. …”

Section: Introductionmentioning

confidence: 99%

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Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Pradeep¹,

Spellman²,

Pratheesh³

et al. 2017

Vet World

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Background and Aim::In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). In this preliminary study, we have proceeded to look for C. burnetii DNA in those antibody positive specimens employing an imported commercial C. burnetii polymerase chain reaction (PCR) kit.Materials and Methods::Blood samples were collected during slaughtering. All 15 blood samples of antibody positive ruminants and three antibody negative samples were subjected to conventional Trans-PCR assay with a commercial PCR kit (Genekam Biotechnology AG, Duisburg, Germany). An in-house Trans-PCR was included in the study for comparison.Results::A total of 15 antibody positive and three antibody-negative serum samples belonging to 11 goat, 4 sheep, 1 cattle, and 2 buffaloes were tested in duplicate for the presence of C. burnetii DNA by the commercial agar gel PCR kit and an in-house Trans-PCR. Only one buffalo serum sample was positive for C. burnetii with a band at 243 bp in in-house Trans-PCR.Discussion::Seropositivity for C. burnetii need not necessarily translate into infectivity status of the animal. Conversely, seronegative ruminants can shed C. burnetii. Rapid disintegration of C. burnetii DNA during the storage period is an important impediment in QF-PCR research. This is the first time the performance of this commercial PCR kit is being validated in India.Conclusion::Commercial PCR kit, Genekam did not identify any positive sample, probably because it targeted a larger amplicon of 687 bp.

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Update on prevalence and distribution pattern of tick-borne diseases among humans in India: a review

Negi

Kandari²,

Arunachalam

2021

Parasitol Res

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First molecular evidence of Coxiella burnetii in patients with atypical pneumonia, India

Cited by 16 publications

References 11 publications

First genetic evidence of Coxiella burnetii in cases presenting with acute febrile illness, India

First genetic evidence of Coxiella burnetii in cases presenting with acute febrile illness, India

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Update on prevalence and distribution pattern of tick-borne diseases among humans in India: a review

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