First Morphological and Molecular Report of<i>Aphelenchoides blastophthorus</i>on Strawberry Plants in Switzerland

Consoli, Erika; Ruthes, Andréa Caroline; Eder, Reinhard; Dahlin, Paul

doi:10.1094/pdis-07-18-1241-re

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Several Aphelenchoides species are known to parasitize or to be associated with strawberry plants: A. besseyi, A. bicaudatus, A. blastophthorus, A. fragariae, A. pseudobesseyi, A. pseudogoodeyi, A. ritzemabosi and A. rutgersi [33][34][35][36][37][38][39]. In this study, A. smolae was reported to be a nematode species associated with strawberry for the first time.…”

Section: Discussionmentioning

confidence: 94%

Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

Subbotin

2024

IJMS

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Rapid and reliable diagnostic methods for plant-parasitic nematodes are critical for facilitating the selection of effective control measures. A diagnostic recombinase polymerase amplification (RPA) assay for Aphelenchoides fragariae using a TwistAmp® Basic Kit (TwistDx, Cambridge, UK) and AmplifyRP® Acceler8® Discovery Kit (Agdia, Elkhart, IN, USA) combined with lateral flow dipsticks (LF) has been developed. In this study, a LF-RPA assay was designed that targets the ITS rRNA gene of A. fragariae. This assay enables the specific detection of A. fragariae from crude nematode extracts without a DNA extraction step, and from DNA extracts of plant tissues infected with this nematode species. The LF-RPA assay showed reliable detection within 18–25 min with a sensitivity of 0.03 nematode per reaction tube for crude nematode extracts or 0.3 nematode per reaction tube using plant DNA extracts from 0.1 g of fresh leaves. The LF-RPA assay was developed and validated with a wide range of nematode and plant samples. Aphelenchoides fragariae was identified from seed samples in California. The LF-RPA assay has great potential for nematode diagnostics in the laboratory with minimal available equipment.

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Section: Discussionmentioning

confidence: 94%

Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

Subbotin

2024

IJMS

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Phylogenetic position of Ptychaphelenchus eucalypticola Hodda, 2009 within the Aphelenchoidoidea Skarbilovich, 1947 (Siddiqi, 1980) inferred from partial 18S and 28S rDNA gene sequences

2022

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Summary At the time of description, the morphology of Ptychaphelenchus eucalypticola Hodda, 2009 indicated it could be assigned to either the Aphelenchoididae Skarbilovich, 1947 (Paramonov, 1953) or the Parasitaphelenchidae Ruehm, 1956 (Siddiqi, 1980) within the Aphelenchoidoidea Skarbilovich, 1947 (Siddiqi, 1980). Although P. eucalypticola was, tentatively, and remains assigned to the Aphelenchoididae, its relationships with other aphelenchoids have not been reassessed, and no molecular data were previously available for this species. We re-collected P. eucalypticola from its type host and locality, Eucalyptus macrorhyncha F. Muell. ex Benth., from Mount Ainslie, ACT, Australia. We performed Bayesian inference and maximum likelihood analyses of a concatenated 18S + 28S rDNA gene sequence dataset to determine the position of P. eucalypticola within the Aphelenchoidoidea, followed by 18S and 28S single-gene analyses to further assess relationships between this species and an expanded set of close relatives. All analyses indicated P. eucalypticola is correctly assigned to the Aphelenchoididae, in a clade comprising all species of Ficophagus Davies & Bartholomaeus, 2015 and some species presently assigned to Aphelenchoides Fisher, 1894, sister to Martininema Davies & Bartholomaeus, 2015 and additional species of Aphelenchoides. Our 18S single-gene analyses did not resolve the position of P. eucalypticola relative to Aphelenchoides and Ficophagus; however, our 28S single-gene analyses indicated a sister relationship between P. eucalypticola and Ficophagus. This sister relationship is plausible as the former species shares many characteristics with species of the latter genus; however, there are sufficient morphological differences to consider P. eucalypticola as representative of a distinct lineage within the Aphelenchoidoidea.

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First report of Robustodorus subtenuis on garlic plants in Poland

Flis,

Dobosz,

Chałańska

et al. 2024

Acta Sci. Pol. Hortorum Cultus

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During the observation of a garlic plantation, a sample consisting of plants with symptoms of growth inhibition and the soil surrounding the roots was collected. Phytophagous nematodes were isolated from the soil sample as well as the garlic plant shoots, with an average density of 75 individuals per 100 mL. Microscope slides were prepared from adult individuals and subjected to morphological and morphometric analysis, as well as DNA examination. It was demonstrated that the examined nematodes belong to the species Robustodorus subtenuis. Both the female and male nematodes differed from individuals previously found in the cultivation of Narcissus and Gladiolus. On average, R. subtenuis females have shorter bodies and longer tails, with the averages calculated for the nematode population from the soil being slightly lower than the average values for nematodes from the garlic shoots. Additionally, the females found in the soil had a shorter distance from the lip region to the vulva and a shorter post-vulval sac, the length of which constituted 32 to 45% of the distance from the vulva to the anus. On average, the male bodies were shorter and had longer tails. They were also wider at the level of the anus. The average body length of males isolated from the soil, like that of the females, was lower than the average for individuals isolated from the garlic shoots. Variations in the influence of the host plant on the morphometry of the parasitic nematodes were observed. This is the first report of R. subtenuis colonizing garlic plants in Poland.

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First Morphological and Molecular Report ofAphelenchoides blastophthoruson Strawberry Plants in Switzerland

Cited by 3 publications

References 24 publications

Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

Phylogenetic position of Ptychaphelenchus eucalypticola Hodda, 2009 within the Aphelenchoidoidea Skarbilovich, 1947 (Siddiqi, 1980) inferred from partial 18S and 28S rDNA gene sequences

First report of Robustodorus subtenuis on garlic plants in Poland

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