First natural crossover recombination between two distinct species of the family Closteroviridae leads to the emergence of a new disease

Ruiz, Leticia; Simón, Almudena; García, Carmen; Velasco, L.; Janssen, Dirk

doi:10.1371/journal.pone.0198228

Cited by 16 publications

(11 citation statements)

References 60 publications

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“…Sequencing of the complete genome revealed that the five conserved nucleotides GAAAT found at the start of the 5′ untranslated regions (UTRs) of RNA1 and RNA2 of the California LCV and of other criniviruses [29,30], were also present at the start of the 5′ UTR of LCV-Can RNA1 and RNA2. However, the 5′ UTRs of LCV-Can showed an additional T at the beginning of the five conserved nucleotides (not attributed to the RACE technique).…”

Section: Resultsmentioning

confidence: 99%

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Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

Hadad

Luria

Smith

et al. 2019

Viruses

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In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV.

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Section: Resultsmentioning

confidence: 99%

“…Whether there is a possible effect of host plants on the infecting crinivirus sequence is an intriguing question. This might occur due to recombination events between criniviruses co-infecting a host [30,52].…”

Section: Discussionmentioning

confidence: 99%

Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

Hadad

Luria

Smith

et al. 2019

Viruses

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“…First, tripartite genome of Potato yellow vein virus contains the gene for the p6 analog in the 3′-terminal region of RNA1 but not in RNA2 as it was found for other criniviruses [119] . Second, the 5′-terminal region of Lettuce chlorosis virus genome contains two short genes coding for hydrophobic proteins, thus it is not clear whether one or both these proteins are required for virus cell-to-cell movement [120] , [121] .…”

Section: Closterovirusesmentioning

confidence: 99%

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes

Morozov

Solovyev

2020

AIMS Microbiology

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Most plant viruses code for movement proteins (MPs) targeting plasmodesmata to enable cell-to-cell and systemic spread in infected plants. Small membrane-embedded MPs have been first identified in two viral transport gene modules, triple gene block (TGB) coding for an RNA-binding helicase TGB1 and two small hydrophobic proteins TGB2 and TGB3 and double gene block (DGB) encoding two small polypeptides representing an RNA-binding protein and a membrane protein. These findings indicated that movement gene modules composed of two or more cistrons may encode the nucleic acid-binding protein and at least one membrane-bound movement protein. The same rule was revealed for small DNA-containing plant viruses, namely, viruses belonging to genus Mastrevirus (family Geminiviridae ) and the family Nanoviridae . In multi-component transport modules the nucleic acid-binding MP can be viral capsid protein(s), as in RNA-containing viruses of the families Closteroviridae and Potyviridae . However, membrane proteins are always found among MPs of these multicomponent viral transport systems. Moreover, it was found that small membrane MPs encoded by many viruses can be involved in coupling viral replication and cell-to-cell movement. Currently, the studies of evolutionary origin and functioning of small membrane MPs is regarded as an important pre-requisite for understanding of the evolution of the existing plant virus transport systems. This paper represents the first comprehensive review which describes the whole diversity of small membrane MPs and presents the current views on their role in plant virus movement.

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“…Due to the importance of P10 and P26 gene orthologues group in pathogenicity, its viral cycle in Crinivirus, and the fact that these genes have not been studied in PYVV, it is necessary to know about the variability, evolution and presence of molecular viral variants in potato plants generated by selection pressure, mutations, genetic drift, migration and also inter and intraspecific recombination events. These factors may impact the PYVV infectious capacity within its host and the ability to colonize other plant species, increasing the hosts' range as in tomato plants (Ruiz et al, 2018). Our objective in this study was to analyze the P26 and P10 genes of PYVV isolates from two contrasting geographic regions of Colombia to determine their genetic variability, evolution, and possible relationships with the host.…”

Section: Introductionmentioning

confidence: 99%

Variability of P26 and P10 genes in Colombian isolates of Potato yellow vein virus (PYVV)

Calderón¹,

Mosquera²,

Vargas³

2019

Agron. colomb.

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Potato yellow vein virus (PYVV) is the causal agent of the potato yellow vein disease and can reduce potato production up to 50%. This virus also infects tomatoes and can remain asymptomatic in plants. PYVV transmission is mediated by vegetative seed, the vector Trialeurodes vaporariorum, and grafts. Its genome has the P26 and P10 genes that are orthologues in the Crinivirus genus, which have been characterized as pathogenic factors and have not been studied in PYVV. We analyzed the variability of P26 and P10 from 45 and 48 sequences, which were obtained by RT-PCR amplification of the total RNA of symptomatic potato leaves from the provinces of Nariño, Cundinamarca, and Boyaca (Colombia). We included sequences of each gene of the PYVV genome of potato and tomato isolates from GenBank. The variability in these genes is influenced by the flow and uncontrolled use of vegetative seed between different provinces, that favor the dispersion of viral variants. In addition, the variability analysis based on maximum likelihood trees, haplotypes, and diversity indices showed that P26 is more variable than P10 and both are more variable in Andigena than in Phureja potatoes. The Tajima and Fu and Li tests revealedthat these genes are subject to negative selection.

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First natural crossover recombination between two distinct species of the family Closteroviridae leads to the emergence of a new disease

Cited by 16 publications

References 60 publications

Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production

Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes

Variability of P26 and P10 genes in Colombian isolates of Potato yellow vein virus (PYVV)

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