First observation of N-acetyl leucine and N-acetyl isoleucine in diabetic patient hair and quantitative analysis by UPLC–ESI–MS/MS

Min, Jun Zhe; Tomiyasu, Yuki; Takashi, Morotomi; Jiang, Yuanliang; Li, Gao; Shi, Qing; Yu, Hai-fu; Inoue, Kōichi; Todoroki, Kenichiro; Toyo’oka, Toshimasa

doi:10.1016/j.cca.2015.02.006

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…In most studies, amino acids from human hair have been extracted in methanol and water, which requires 12 hours. 42 To improve the success of dl -AA extraction from hair, this study applied a pre-extraction treatment in different solvents (methanol and water). The extraction temperature was 50 °C, and the dl -AA extraction time was 12 hours.…”

Section: Resultsmentioning

confidence: 99%

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Development and evaluation of a novel fluorescent chiral derivatization reagent DBD-S-M-Pro: first observation of four chiral amino acids in human hair

et al. 2023

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Section: Resultsmentioning

confidence: 99%

“…In most studies, amino acids from human hair have been extracted in methanol and water, which requires 12 hours. 42 To improve the success of The amino acids DL-Glu, Ala, Val, and Phe were extracted from the hair of healthy volunteers. Fig.…”

Section: Optimization Of the Extraction Conditions Of Dl-amino Acids ...mentioning

confidence: 99%

Development and evaluation of a novel fluorescent chiral derivatization reagent DBD-S-M-Pro: first observation of four chiral amino acids in human hair

et al. 2023

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“…It is further known that the basic organic compounds are efficiently incorporated into the hair shaft based on the strong affinity with the melanin pigment. [106][107][108][109] Indeed, histamine, polyamines and their metabolites were detected in mammalian hairs by LC-MS. [110][111][112][113] These results suggest that various metabolites intrinsically exist in the hairs. Based on the concept, we searched for molecules related to diseases in human and animal hairs.…”

Section: Hair Analysismentioning

confidence: 94%

Diagnostic Approach to Disease Using Non-invasive Samples Based on Derivatization and LC-ESI-MS/MS

Toyo’oka

2016

Biological & Pharmaceutical Bulletin

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The determination of biologically-active molecules is very important in order to understand biological functions. A novel approach for the highly sensitive and specific determination seems to be essential for this purpose. Based on this consideration, we synthesized various types of fluorogenic and fluorescent reagents for the derivatization of chiral and achiral molecules. The fluorescence analysis is excellent for the analysis of target molecules and generally provides good expected results. However, the trace analysis of the bioactive molecules in complex matrices, such as plasma and urine, is not always satisfactory even using high-performance fluorometry. In such a situation, mass spectrometry (MS) is another technique for the selective and sensitive determination of biological components. Therefore, various derivatization reagents for MS/MS detection were developed and used for the determination of amines and carboxyls including chiral molecules. These newly developed reagents were also adopted for the biomarker detection related to diseases using non-invasive samples (i.e., saliva, nail, hair). Although the determination of the targeted chiral molecules is relatively easy, it is very difficult to identify and/or determine the enantiomeric biomarker in real samples. To solve this difficulty, we proposed the strategy called "chiral metabolomics," which means the total analysis of the enantiomers of various chiral metabolites in complex matrices. This review paper focused on the development of various new derivatization reagents for amines and carboxyls by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and the detection of the biomarker candidates related to several diseases in non-invasive samples (i.e., hair, nail, saliva) using these reagents.

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“…The reagents described in the previous sections are useful for the sensitive and specific analysis of amines and carboxylic acids by fluorometry [58][59][60][61][62][63] and MS. [7][8][9][10][11][12][13] However, the determination of each enantiomer of chiral molecules is essentially impossible because there is no chiral recognition moiety in the reagent structures. Therefore, chiral-derivatization reagents for the separation and detection of a pair of enantiomers were also developed in our laboratories (Fig.…”

Section: Reagents For Chiral Amines and Carboxylic Acidsmentioning

confidence: 99%

“…Mass spectrometry (MS) is an alternative technique for the selective and sensitive determination of biological components, [7][8][9][10][11][12][13] the same as fluorescence analysis. MS instrumentation has gradually decreased and the analytical software is also good enough for efficient determinations.…”

Section: Introductionmentioning

confidence: 99%

Derivatization-based High-throughput Bioanalysis by LC-MS

Toyo’oka

2017

ANAL. SCI.

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Liquid chromatography coupled with mass spectrometry (LC-MS) is one of the most prominent analytical techniques due to its inherent selectivity and sensitivity. LC-MS is currently the first choice for high-throughput bioanalysis due to the advancements in MS instruments and the analytical software. Based on this situation, we are developing various types of derivatization reagents, including chiral reagents for MS and/or MS/MS detection. These developed reagents are adopted for the detection of biomarker candidates related to diseases. The biomarker candidates include not only achiral molecules, but also chiral ones. Although determining the already-identified chiral molecules is relative easy, it is very difficult to identify and/or determine unknown enantiomer(s) in real samples. To solve this difficulty, we proposed a new strategy to identify unknown enantiomeric biomarkers related to diseases. This review paper deals with the development of derivatization reagents for amines and carboxylic acids in LC-MS analysis and their application to bioanalysis.

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First observation of N-acetyl leucine and N-acetyl isoleucine in diabetic patient hair and quantitative analysis by UPLC–ESI–MS/MS

Cited by 8 publications

References 30 publications

Development and evaluation of a novel fluorescent chiral derivatization reagent DBD-S-M-Pro: first observation of four chiral amino acids in human hair

Development and evaluation of a novel fluorescent chiral derivatization reagent DBD-S-M-Pro: first observation of four chiral amino acids in human hair

Diagnostic Approach to Disease Using Non-invasive Samples Based on Derivatization and LC-ESI-MS/MS

Derivatization-based High-throughput Bioanalysis by LC-MS

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