First Occurrence of Phytoplasma in Rhododendron Sp. In the Czech Republic

Mertelik, J.; Kloudova, K.; Vanc, P.; Mokrá, V.; Šedivá, J.; Navrátil, Milan; Válová, P.

doi:10.17660/actahortic.2006.722.45

Cited by 3 publications

(6 citation statements)

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“…() and Mertelík et al. (), originated from a breeding programme at the Silva Tarouca RILOG Průhonice. The phytoplasma diseases found in the Czech Republic differed from one another, as they induced different symptoms.…”

Section: Resultsmentioning

confidence: 99%

“…The stolbur‐type rhododendron plants exhibited leaf malformation and variegation (Mertelík et al. ). The aster yellows type here described was characterized by witches’ broom growth, malformation, mosaic and necrosis of leaves.…”

Section: Resultsmentioning

confidence: 99%

“…Stolbur‐type phytoplasma was detected using nested polymerase chain reaction (PCR) and identified by restriction fragment length polymorphism (RFLP) (Mertelík et al. ). The natural occurrence of ‘ Candidatus Phytoplasma trifolii’ in Rhododendron hybridum plants was revealed by transmission electron microscopy (TEM), as well as PCR and RFLP analysis (Přibylová et al.…”

Section: Introductionmentioning

confidence: 99%

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Aster Yellows Subgroup 16SrI‐C Phytoplasma in Rhododendron hybridum

Přibylová

Špak

Fráňová

2013

Journal of Phytopathology

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Symptoms of unknown aetiology on Rhododendron hybridum cv. Cunningham's White were observed in the Czech Republic in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma‐like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma‐specific, universal and group‐specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma into the aster yellows subgroup I‐C. Sequence analysis of the 16S‐23S ribosomal operon of the amplified phytoplasma genome from the infected rhododendron plant (1724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. These data suggest Rhododendron hybridum is a new host for the aster yellows phytoplasma subgroup 16SrI‐C in the Czech Republic and worldwide.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aster Yellows Subgroup 16SrI‐C Phytoplasma in Rhododendron hybridum

Přibylová

Špak

Fráňová

2013

Journal of Phytopathology

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“…The first phytoplasma-like symptoms were observed in the rhododendron plants 'Cunningham's White' in the Czech Republic in 1997; these symptoms included leaf malformation and variegation. Phytoplasma was detected using nested PCR and identified by restriction fragment length polymorphism (RFLP) as stolbur-type phytoplasma (Mertelík et al 2006).…”

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confidence: 99%

“…All rhododendron plants mentioned in this study (as in the study by Mertelík et al 2006) originated from a breeding programme at the RILOG Průhonice Fig. 4 Phylogenetic tree constructed using the analysis of partial 16S rRNA sequences.…”

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confidence: 99%

The first detection of ‘Candidatus Phytoplasma trifolii’ in Rhododendron hybridum

2008

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Four Rhododendron hybridum plants (from cvs Moravanka and Don Juan), all exhibited symptoms of shortened axillary shoots, reduced leaves with vein clearing and yellowing, undeveloped flowers, and general stunting in a rhododendron nursery garden in southern Bohemia in 2007. Electron microscopy examination of ultra-thin sections revealed the presence of numerous polymorphic phytoplasma-like bodies in the phloem tissue of leaf midribs and petioles. The phytoplasma etiology of this disease was further confirmed by polymerase chain reaction (PCR) using universal phytoplasma primers. Restriction fragment length polymorphism (RFLP) analysis of amplification products obtained with a R16F2/R16R2 primer pair from all symptomatic plants indicated the presence of phytoplasma from the 16SrVI-A subgroup. A detailed comparison of the amplified sequences and phylogenetic analysis confirmed that the phytoplasma belonged to the subgroup 16SrVI-A (clover proliferation phytoplasma group). This is the first report of the natural occurrence of 'Candidatus Phytoplasma trifolii' in plants of Rhododendron hybridum.

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Resistance of horse chestnut tree (variety ‘Mertelík’) to Cameraria ohridella Deschka & Dimić, 1986 (Lepidoptera: Gracillariidae)

Gubka,

Zúbrik,

Mertelík

et al. 2024

Central European Forestry Journal

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Resistance of horse chestnut Aesculus hippocastanum ‘Mertelík’ to Cameraria ohridella was proven in the trial under semi-field conditions. Seedlings of vulnerable and resistant trees were grown together in rearing cages and were exposed to the strong attack from C. ohridella larvae. Significant differences in leaflet injuries were observed between susceptible and insect-resistant trees. Variety ‘Mertelík’ showed almost none or very small damages compared to high defoliation level in case of standard trees. The results confirmed that this resistant clone could be suitable for use under field conditions.

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First Occurrence of Phytoplasma in Rhododendron Sp. In the Czech Republic

Cited by 3 publications

References 0 publications

Aster Yellows Subgroup 16SrI‐C Phytoplasma in Rhododendron hybridum

Aster Yellows Subgroup 16SrI‐C Phytoplasma in Rhododendron hybridum

The first detection of ‘Candidatus Phytoplasma trifolii’ in Rhododendron hybridum

Resistance of horse chestnut tree (variety ‘Mertelík’) to Cameraria ohridella Deschka & Dimić, 1986 (Lepidoptera: Gracillariidae)

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