First record of gregarines (Apicomplexa) in seminal vesicle of insect

Dias, Glenda; Dallai, Romano; Carapelli, Antonio; Almeida, João Fernando Lourenço de; Campos, Lúcio Antônio de Oliveira; Faroni, L. R. D.; Lino‐Neto, José

doi:10.1038/s41598-017-00289-3

Cited by 12 publications

(11 citation statements)

References 32 publications

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“…We also identified a small cluster containing only one contig matching to a recorded genome sequence, Apicystis bombi , a gregarine known to parasitise honey bees 65 . To identify the exact species present, we sequenced the PCR results of custom primers against the largest contig in this cluster, in conjunction with primers encompassing the 18 S and ITS2 rDNA regions, as used by Dias et al for the characterisation of novel gregarine species 66 (Supplementary Fig. 5d ).…”

Section: Resultsmentioning

confidence: 99%

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Characterisation of the British honey bee metagenome

et al. 2018

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The European honey bee (Apis mellifera) plays a major role in pollination and food production. Honey bee health is a complex product of the environment, host genetics and associated microbes (commensal, opportunistic and pathogenic). Improved understanding of these factors will help manage modern challenges to bee health. Here we used DNA sequencing to characterise the genomes and metagenomes of 19 honey bee colonies from across Britain. Low heterozygosity was observed in many Scottish colonies which had high similarity to the native dark bee. Colonies exhibited high diversity in composition and relative abundance of individual microbiome taxa. Most non-bee sequences were derived from known honey bee commensal bacteria or pathogens. However, DNA was also detected from additional fungal, protozoan and metazoan species. To classify cobionts lacking genomic information, we developed a novel network analysis approach for clustering orphan DNA contigs. Our analyses shed light on microbial communities associated with honey bees and demonstrate the power of high-throughput, directed metagenomics for identifying novel biological threats in agroecosystems.

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Section: Resultsmentioning

confidence: 99%

“…Primers were run through NCBI BLAST to confirm specificity 99 . Apicystis bombi specific primers were used as described in Dias et al 66 . Specific primers against Nosema ceranae were used as described by Chen et al 104 and Lotmaria passim specific primers were used as described by Stevanovic et al 82 .…”

Section: Methodsmentioning

confidence: 99%

Characterisation of the British honey bee metagenome

et al. 2018

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“…2). Interestingly, Module#3 also included arachnid species, a group of invertebrates that have recently been reported to be parasitized by Gregarina species (Dias et al 2017). Gregarina spp.…”

Section: Discussionmentioning

confidence: 99%

“…are often found to be a parasite of soil invertebrates including arthropoda, and annelids (Omoto and Cartwright 2003). Interestingly, Module#3 also included arachnid species, a group of invertebrates that have recently been reported to be parasitized by Gregarina species (Dias et al 2017). Furthermore, Module#4, abundant in high latitudes included several phylotypes from phylum Ciliophora (Protozoa), a group of organisms that is well-known to feed on bacteria, an interaction that might allow phylum Ciliophora to colonize the thrive under the extreme conditions found in Antarctica.…”

Section: Discussionmentioning

confidence: 99%

Ecological drivers of soil microbial diversity and soil biological networks in the Southern Hemisphere

Delgado‐Baquerizo

Reith

Dennis

et al. 2018

Ecology

182

100

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The ecological drivers of soil biodiversity in the Southern Hemisphere remain underexplored. Here, in a continental survey comprising 647 sites, across 58 degrees of latitude between tropical Australia and Antarctica, we evaluated the major ecological patterns in soil biodiversity and relative abundance of ecological clusters within a co-occurrence network of soil bacteria, archaea and eukaryotes. Six major ecological clusters (modules) of co-occurring soil taxa were identified. These clusters exhibited strong shifts in their relative abundances with increasing distance from the equator. Temperature was the major environmental driver of the relative abundance of ecological clusters when Australia and Antarctica are analyzed together. Temperature, aridity, soil properties and vegetation types were the major drivers of the relative abundance of different ecological clusters within Australia. Our data supports significant reductions in the diversity of bacteria, archaea and eukaryotes in Antarctica vs. Australia linked to strong reductions in temperature. However, we only detected small latitudinal variations in soil biodiversity within Australia. Different environmental drivers regulate the diversity of soil archaea (temperature and soil carbon), bacteria (aridity, vegetation attributes and pH) and eukaryotes (vegetation type and soil carbon) across Australia. Together, our findings provide new insights into the mechanisms driving soil biodiversity in the Southern Hemisphere.

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“…Ileal mucosal specimens fixed in 2.5% glutaraldehyde were post-fixed, dehydrated and embedded in epoxy resin. Ultrathin sections were made and post-stained in uranyl acetate and lead citrate 56 . The ileal mucosal ultrastructure was analysed on a Philips Tecnai 10 electron microscope (Philips, Eindhoven, the Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Bifidobacterium pseudocatenulatum LI09 and Bifidobacterium catenulatum LI10 attenuate D-galactosamine-induced liver injury by modifying the gut microbiota

Fang

Shi

et al. 2017

Sci Rep

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The gut microbiota is altered in liver diseases, and several probiotics have been shown to reduce the degree of liver damage. We hypothesized that oral administration of specific Bifidobacterium strains isolated from healthy guts could attenuate liver injury. Five strains were tested in this study. Acute liver injury was induced by D-galactosamine after pretreating Sprague-Dawley rats with the Bifidobacterium strains, and liver function, liver and ileum histology, plasma cytokines, bacterial translocation and the gut microbiome were assessed. Two strains, Bifidobacterium pseudocatenulatum LI09 and Bifidobacterium catenulatum LI10, conferred liver protection, as well as alleviated the increase in plasma M-CSF, MIP-1α and MCP-1 and bacterial translocation. They also ameliorated ileal mucosal injury and gut flora dysbiosis, especially the enrichment of the opportunistic pathogen Parasutterella and the depletion of the SCFA-producing bacteria Anaerostipes, Coprococcus and Clostridium XI. Negative correlations were found between MIP-1α / MCP-1 and Odoribacter (LI09 group) and MIP-1α / M-CSF and Flavonifractor (LI10 group). Our results indicate that the liver protection effects might be mediated through gut microbiota modification, which thus affect the host immune profile. The desirable characteristics of these two strains may enable them to serve as potential probiotics for the prevention or adjuvant treatment of liver injury.

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First record of gregarines (Apicomplexa) in seminal vesicle of insect

Cited by 12 publications

References 32 publications

Characterisation of the British honey bee metagenome

Characterisation of the British honey bee metagenome

Ecological drivers of soil microbial diversity and soil biological networks in the Southern Hemisphere

Bifidobacterium pseudocatenulatum LI09 and Bifidobacterium catenulatum LI10 attenuate D-galactosamine-induced liver injury by modifying the gut microbiota

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