First Record of<i>Chrysomya megacephala</i>(Fabricius, 1794) (Diptera: Calliphoridae) in Algeria

Taleb, Meriem; Tail, Ghania; Açikgöz, Halide Nihal

doi:10.3157/021.128.0112

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…Adult flies were collected during spring (between 21 April and 8 May) of 2016 on 18 exposed male rabbit carcasses weighing approximately (2.50 ± 0.42) kg in a semi-urban site in Blida, Algeria (coordinates: 36° 30′ 38.5″ N, 2° 52′ 21.4″ E) at 188 m above sea level using a trap described in Taleb et al [ 24 ]. The carcass and the trap were protected by a metal cage measuring 80 cm × 60 cm × 150 cm.…”

Section: Methodsmentioning

confidence: 99%

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Taleb

Tail

Açikgöz

2021

Forensic Sciences Research

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Cluster flies are represented by the genus Pollenia Robineau-Desvoidy, 1830 of the family Polleniidae Brauer and Bergenstamm, 1889. Their larvae are known to be internal parasites or predators of earthworms. Herein, we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius, 1794 and Pollenia vagabunda (Meigen, 1826) (Diptera: Polleniidae) on carcasses in Algeria and identify them through DNA barcoding. A region of the mitochondrial cytochrome c oxidase I gene ( COI ) was amplified and sequenced. Genetic distances were determined. A phylogenetic tree was constructed with the maximum parsimony method using 10 000 bootstrap replicates. A total number of 157 adults of P. rudis were collected together with 325 adults of Pollenia vagabunda . The occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of decomposition. All the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification engine. Intra- and interspecific sequence divergence values were less than 1% and greater than 3%, respectively. COI barcodes obtained from this study were robust enough to identify and distinguish unambiguously between P. rudis and P. vagabunda . In the tree-based analysis, the cluster flies were all assigned to their respective species separately from each other confirming the morphological identification. These results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.

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Section: Methodsmentioning

confidence: 99%

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Taleb

Tail

Açikgöz

2021

Forensic Sciences Research

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“…Adult flies were captured using baited traps that have been previously described 37,38 . After trapping, the flies were sorted, and the green bottle fly, L. sericata , was selected for this study due to its widespread use in MDT for decades and its large distribution and abundance 37 .…”

Section: Methodsmentioning

confidence: 99%

“…Adult flies were captured using baited traps that have been previously described. 37,38 After trapping, the flies were sorted, and the green bottle fly, L. sericata, was selected for this study due to its widespread use in MDT for decades and its large distribution and abundance. 37 Each specimen was morphologically identified using the dichotomous key described by Szpila 39 under Carl Zeiss ® Stemi 2000-C stereo zoom microscope coupled with a digital camera.…”

Section: Rearing and Egg Collectionmentioning

confidence: 99%

Effectiveness of broad‐spectrum antiseptics in production of disinfected maggots of Lucilia sericata for use in wound debridement therapy

Taleb

2021

Wound Repair Regeneration

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The establishment of low-cost, effective, safe and practical methods is necessary to increase the use of larval therapy in wound care. Although studies on external disinfection of calliphorid eggs have been reported, many studies lack data on the effect of disinfection on egg viability and the microorganisms found before disinfection.Therefore, the main objective of this study was to compare three antiseptic solutions, that is, chlorhexidine (5%), Dakin's solution (0.5% NaOCl) and povidone-iodine (10%), in terms of their ability to disinfect Lucilia sericata eggs. Egg viability after disinfection and microorganisms present on the eggs and larvae before and after treatment were also examined. None of the antiseptics had a significant effect on egg viability. Disinfection of L. sericata eggs with 0.5% NaOCl was the best method, as sterility tests showed no contamination. Escherichia coli, Bacillus subtilis and Proteus mirabilis were present in all cultures isolated from the non-disinfected eggs and larvae, while Enterococcus faecium, Enterococcus faecalis, Morganella morganii, Corynebacterium spp. and Providencia stuartii were isolated from more than half of the same cultures. Sterility testing of medicinal maggots after disinfection is crucial to prevent secondary infections and achieve a positive therapeutic outcome.

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DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps

2019

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First Record ofChrysomya megacephala(Fabricius, 1794) (Diptera: Calliphoridae) in Algeria

Cited by 6 publications

References 16 publications

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Effectiveness of broad‐spectrum antiseptics in production of disinfected maggots of Lucilia sericata for use in wound debridement therapy

DNA barcoding of Stearibia nigriceps (Meigen) and Piophila casei (Linnaeus) (Diptera: Piophilidae) from Algeria and the first African report of Stearibia nigriceps

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